Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"Marine bacterium"”.
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Mutant analysis of luminescence and autoinduction in a marine bacterium
The marine symbiotic bacterium Vibrio fischeri is striking for its ability both to emit light and to dramatically regulate light emission using a cell-to-cell signalling mechanism called autoinduction. The latter is mediated by a signal molecule called the "autoinducer". The mechanistic bases of …
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Mutant analysis of luminescence and autoinduction in a marine bacterium
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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A Study of the Inactivation in Vivo of the Luciferase in the Luminous Marine Bacterium Beneckea Harveyi
… in stationary phase cultures of the luminous marine bacterium Beneckea harveyi was studied in an effort to elucidate its mechanism. Through studies utilizing inhibitors of metabolic energy production, an inhibitor of protein synthesis, and a mutant in which the luciferase was inactivated at a …
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A Genetic and Physiological Study of the Role of Extracellular Copper-Binding Proteins in Copper Detoxification by the Marine Bacterium <i>Vibrio alginolyticus</i>
… each isolate ten times on nonselective marine agar (10° MA), and comparing plate counts on unamended and 40μM Cu-amended agar to corresponding plate counts of isolates freshly passed on Cu-amended agar.</p> <p>One Cu<sup>r</sup> isolate, Cu40B3, constitutively produced a ca. 21 kDa …
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The role of melanin production in the survival of Vibrio cholerae in the marine environment
The marine bacterium Vibrio cholerae produces pyomelanin through the catabolism of L-tyrosine to homogentisic acid. Various types of melanins are used by microbes as defense mechanisms against a variety of environmental stresses. This thesis investigated the defensive role of V. cholerae pyomelanin …
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A Proteomics Approach to the Examination of Proteins in Marine Systems
… to a more thorough understanding of the marine carbon and nitrogen cycles. The examination of molecular organic biomarkers throughout an Arctic sediment core showed the dominant input in the area was from marine sources with lower but steady contributions from terrestrial sources during …
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Over-expression and characterization of four alginate lyases from vibrio splendidus 12B01
… polyG, polyM, or polyMG block degradation. The marine bacterium Vibrio splendidus 12B01 contains four putative alginate lyases which were investigated in this study. We identified, purified, and characterized the four PL7 alginates lyases. We found that these lyases have optimal activity between …
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Exploring the optimality of various bacterial motility strategies : a stochastic hybrid systems approach
… complicated run-reverse-flick strategy of the marine bacterium Vibrio alginolyticus. We use stochastic stability theory to analyze the chemotaxis models in terms of their stationary distributions and also derive a diffusion approximation of the system that provides further insight into the …
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Production and application of antibodies produced against the flagella and hemolysin of Vibrio parahaemolyticus
Vibrio parahaemolyticus is a marine bacterium which is the leading cause of bacterial gastroenteritis due to consumption of raw or undercooked bivalve molluscan shellfish in United States and is considered an important seafood-borne pathogen throughout the world. The conventional methods for …
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The agarase system of saccharophagus degradans strain 2-40 : analysis of the agarase system and protein localization
… degradans") strain 2-40 is a Gram-negative marine bacterium isolated from the Chesapeake Bay. Analysis of 16s rDNA sequence indicated that S. degradans is related to a group of marine proteobacteria adept at degrading complex polysaccharides (CPs). S. degradans can depolymerize at least ten …
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Characterization of VIBRIO Parahaemolyticus-Induced Intestinal Inflammation in the Mouse
The Gram-negative marine bacterium Vibrio parahaemolyticus is a leading cause of gastroenteritis from the consumption of contaminated seafood. Some recent outbreaks are attributed to a rise in ocean temperatures, a trend that is likely to continue as a result of global warming and emphasizes the …
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Linking microbial metabolism and organic matter cycling through metabolite distributions in the ocean
Key players in the marine carbon cycle are the ocean-dwelling microbes that fix, remineralize, and transform organic matter. Many of the small organic molecules in the marine carbon pool have not been well characterized and their roles in microbial physiology, ecological interactions, and carbon …
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Characterization of the Type III Effector VOPA from Vibrio Parahaemolyticus
Vibrio parahaemolyticus is a marine bacterium and causative agent of gastroenteritis associated with the consumption of contaminated seafood. It is endemic to Southeast Asia and is the leading cause of gastroenteritis in Japan. Sequencing of the Vibrio parahaemolyticus genome revealed the presence …
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Synthesis and characterization of tethered poly(n-isopropylacrylamide) for quantitative cell adhesion studies
… ATRP. A quantitative study of detachment of marine bacteria from these surfaces, both above and below the LCST using a spinning disc apparatus is also reported. This device applies a linear range of reproducible shear forces to cells attached at the interface of a single sample. In this work, …
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Metabolomics as a tool in the identification and production of new marine-derived antibiotics from sponges and endosymbiotic bacteria
… antibiotics and pharmacologically-active drugs. Marine sponges and their endosymbionts have proven to be abundant sources of bioactive compounds. HR-LCFTMS and NMR were used in the identification of compounds isolated from a marine bacterium and its host sponge, as well as in the dereplication …
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Linking microbial metabolism and organic matter cycling through metabolite distributions in the ocean
Key players in the marine carbon cycle are the ocean-dwelling microbes that fix, remineralize, and transform organic matter. Many of the small organic molecules in the marine carbon pool have not been well characterized and their roles in microbial physiology, ecological interactions, and carbon …
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Investigating bacterial adhesion proteins as tools for controlling bacteria-surface interactions
… (RTX) adhesin in the Gram-negative marine bacterium Marinomonas primoryensis, I was able to prevent their binding to a photosynthetic diatom with which the bacteria form symbiotic biofilms underneath sea ice. The sugar-binding domain was optimally blocked with the monosaccharide …
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Signaling between Two Sensor Kinases Controls Biofilms and Host Colonization in a Bacterial Symbiont
… effector (the response regulator, RR). The marine bacterium Vibrio fischeri uses an elaborate TCS phosphorelay containing a hybrid SK, RscS, and two RRs, SypE and SypG, to colonize its natural squid host, Euprymna scolopes. This TCS pathway regulates V. fischeri's ability to form a biofilm, …
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The quorum-sensing regulation of Vibrio fischeri : novel components of the autoinducer/LuxR regulatory circuit
In the marine bacterium Vibrio fischeri two intercellular homoserine-Iactone signal molecules (luxI-dependent 30C6-HSL and the ainS-dependent C8-HSL) and the transcriptional activator LuxR regulate the luminescence system in a cell-density dependent manner by a process termed quorum sensing. In …
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