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Showing 1 to 11 of 11 for “"Manganese peroxidase"”.
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The Protein Redesign Approach to Modeling Manganese Peroxidase
The active site tryptophans were removed from MnCcP in order to investigate the roles of Trp51 and Trp191 in MnP activity. These residues are phenyalanines in MnP. In CcP mutants without the Mn(II)-binding site, Trp191 and Trp51 greatly influence the lifetime of the porphyrin pi-cation radical. The …
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Removal of refractory contaminants from mature landfill leachate utilizing fungi and evaluation of lignocellulosic enzymatic activity
… and associated highest laccase (Lac), lignin-peroxidase (LiP), and manganese-peroxidase (MnP) activities of 250, 8, and 11 U/L from Penicillium sp. and 165, 19, and 27 U/L from T. chioneus. Penicillium sp. was also utilized in a continuous pack bed bioreactor operated at 48, 72, 96, and 120 h …
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Renewable Natural Polymer Thin Films and Their Interactions with Biomacromolecules
… catalyzed by physically immobilized horseradish peroxidase was investigated using QCM-D and AFM. Results from QCM-D and AFM showed that coniferyl and p-coumaryl alcohol underwent polymerization directly, whereas sinapyl alcohol required the addition of a nucleophile for polymerization. Studies by …
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Performance Enhancement of Aerobic Landfill Bioreactors
… in a waste matrix. The results showed that Manganese Peroxidase (MnP) is the best enzyme for lignin degradation. The enzymatic enhancement of leachate with MnP resulted in rapid hydrolysis of lignocellulose. Also, enzymes were able to increase the overall reaction rates. However, the overall …
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Application of bacterial ligninase enzymes from rynchophorus ferrugineus’s gut consortium in agropulp biodelignification
… The production of ligninase enzymes (lignin peroxidase, manganese peroxidase and laccase) in all species were optimised using response surface methodology (RSM). The results revealed maximal production of ligninase is achieved at optimal pH of 5 to 6 and optimal temperature of 40 °C after 7 …
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Evaluation of ligninolytic enzymes of (coptotermes curvignathus) gut bacteria for oil palm residues biodelignification
… a novel lignin degrader showed highest manganese peroxidase (76.36 ± 15.74 U/L) and laccase activity (70.67 ± 16.82 U/L), while maximal activity of lignin peroxidase (262.49 ± 0.92 U/L) was recorded in culture supernatants of Bacillus cereus. Empty fruit bunch (EFB) had the highest …
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Isolating, characterizing, and engineering novel Cu-proteins and peroxidases
… in one of our model scaffolds, cytochrome c peroxidase (CcP). Chapter 7 describes the work done to enhance the Mn(II) oxidation activity in a designed model of manganese peroxidase within the CcP scaffold based on modifications of the second coordination sphere around the Mn(II) binding site. …
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Microbial Pretreatment of Cotton Stalks by Phanerochaete chrysosporium for Bioethanol Production
… enzyme assays failed to detect lignin peroxidase (LiP) and manganese peroxidase (MnP) activities in fungal pretreatment cultures. Efforts were made to overcome the drawbacks of standardized assays by performing protein gel electrophoresis and crude enzyme delignification studies. …
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Supported Metalloporphines as novel and bioinspired Lignolytic Peroxidase-like Catalysts
… strictly emulate the active site of ligninolytic peroxidases, their use in delignification processes has been presented and future trends outlined. In order to achieve a structural emulation, several coordinating groups have been used to coordinatively immobilize metalloporphines. Synthesized …
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The physiological response of the white-rot fungus, Schizophyllum commune to Trichoderma Viride, during interspecific mycelial combat
… of phenol-oxidising enzymes, namely laccase and manganese peroxidase increased in the confronted mycelial domain of S. commune. Chitinase activity increased in mixed liquid cultures of both fungi. Protein, and gene expression patterns, in the confronted mycelia of S. commune suggest an increase …
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Creation of a Binary CuSO4 Inducible gene expression system in Arabidopsis thaliana and its application to the degradation of Lignin in planta
Lignin is a complex, highly aromatic and heterogenous plant polymer present in lignocellulosic biomass, and is second only to cellulose as the most abundant biopolymer on earth, representing the largest naturally occuring renewable source of aromatics. However, the remarkable recalcitrance of …