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Showing 1 to 5 of 5 for “"ManNAc"”.
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Characterization and improvement of interferon-[gamma] glycosylation in Chinese hamster ovary cell culture
… minimized through feeding of Nacetylmannosamine (ManNAc), a synthetic orecursor of sialic acid. Feeding of 20 mM ManNAc reduced the proportion of undersialylated glycan structures by 50%. Radiolabeled MarNAc was utilized to confirm that the supplemental ManNAc was incorporated into IFNY-[gamma] as …
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High-throughput quantification of glycoprotein sialylation
… we used the HTM to study the effects of feeding ManNAc, Cu 2+, and Mn2+ on sialylation of glycoproteins produced in CHO-IFN-y cell cultures. We found that feeding these chemicals increased sialylation from 20 to 36 mg sialic acid/g protein in batch CHO cell cultures. Moreover, a quadratic least …
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Biochemical and biophysical investigations of N-linked glycosylation pathways in archaea
… and AgIA) to afford Dol-P-GlcNAc- Glc-2,3-diNAcA-ManNAc(6Thr)A. Despite the homology to other bacterial or eukaryotic OTases that exploit polyprenyl-PP-linked substrates, the M. voltae AglB efficiently transfers disaccharide to model peptides from the Dol-P-GlcNAc-Glc-2,3-diNAcA monophosphate. …
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Investigation of asparagine-linked glycosylation in archaeal and bacterial systems
… produces UDP-GlcNAc(3NAc)A as a precursor to ManNAc(3NAc)A, a carbohydrate found in the O-antigen of the lipopolysaccharide of the organism. Using a combination of protein biochemistry and NMR spectroscopy, three enzymes (WbpB, WbpE, and WbpD) were confirmed to be responsible for the …
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Mechanisms of polyphosphate degradation
The goal of my research has been to investigate the mechanisms by which inorganic polyphosphate (polyP) is degraded in vivo. PolyP is a linear chain of phosphate moieties linked through high-energy phosphoanhydride bonds and can range in size from three-phosphates-long to thousands. Naturally, …