Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 95 for “"Mammary epithelial cells"”.
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Xenogeneic transplantation of immortalized bovine mammary epithelial cells
… to investigate the use of an immortalized bovine mammary epithelial cell line as a starting material for xenogeneic transplantation into the mammary glands of immunocompetent recipients. PSG-5 cells (a clonal derivative of the MAC-T cell line engineered to express ovine IGF-I ) were transplanted …
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THE ROLE OF ROMA IN MOUSE MAMMARY EPITHELIAL CELLS
Epithelial to Mesenchymal Transition (EMT) and its reversal, via Mesenchymal to Epithelial Transition (MET), describes the highly coordinated cycle of epithelial plasticity by which normal tissues are remodelled and repositioned during development. Previous studies have characterised the role of …
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Differentiation dynamics of mammary epithelial cells during normal and malignant development
The mammary gland has been central to the evolution of mammals by being capable of undergoing multiple rounds of substantial remodelling during pregnancy and providing nourishment to the offspring. This plasticity does not come without a cost. 1 in 7 females will be diagnosed with breast cancer in …
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Cationic liposome-mediated transfection of mammary epithelial cells with AAV-based plasmid DNA
… techniques for transfecting adult somatic mammary epithelial cells with the aim of producing non-germline transgenic animals. Initially, chemical-mediated techniques were used to deliver plasmid DNA to mammary epithelial cells in vitro: this work was subsequently extended to in vivo …
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Identification of molecular targets regulating fatty acid synthesis in bovine mammary epithelial cells
… that regulate fatty acid synthesis in bovine mammary epithelial cells. The regulation of lipogenic genes including acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) is controlled by transcription factors including sterol regulatory element binding protein-1 (SREBP1) and liver X …
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Regulation of sterol regulatory element binding protein-1 in bovine mammary epithelial cells
… (SCD) were first validated in a bovine mammary epithelial cell line (MacT) and in bovine mammary tissues. At least one or two SRE binding sites in 24 selected lipogenic genes were identified within 50,000 base pair to the 5'-transcription start site through ChIP-seq. The genes closest to …
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Effects of DDT and DDE on gap junctional intercellular communication in human mammary epithelial cells
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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HUMAN CLCA2 IS A P53-DEPENDENT ZINC METALLOPROTEASE AND ITS INTERACTION WITH EVA1 MAINTAINS DIFFERENTIATION OF HUMAN MAMMARY EPITHELIAL CELLS
… or purified protein from CLCA2-transfected cells, we show here that CLCA2 cleavage is catalyzed by zinc and inhibited by metal chelator EDTA. Moreover, an E165Q mutation in the metal binding site abolished processing without affecting stability or trafficking. The mutant could be cleaved by …
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Effects of ovarian steroids on bovine mammary epithelial cells: in vitro and in viro evidence of indirect stimulation of proliferation
… the effects of ovarian steroids on bovine mammary epithelial cell proliferation. In a first study, estrogen (E), progesterone (P), or E+P were administered to prepubertal beef heifers and biopsied mammary parenchyma taken before and following treatment were compared for growth by evaluation …
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Peptides as amino acid sources for the synthesis of secreted proteins by mammary tissue explants and cultured mammary epithelial cells
… for the synthesis of secreted proteins. Mammary tissue explants from lactating (10 to 11 d) CD-1 mice and cultured bovine mammary epithelial cells (MAC-T) were used as experimental models. Explants and cultured cells were incubated at 37°C in a humidified atmosphere of 90% air/10% CO₂ and …
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Investigating milk cell population changes in response to heat stress and characterizing functionality of epithelial cells in milk of lactating dairy cows
Understanding the number of mammary epithelial cells a cow maintains is essential to maximize milk synthesis in lactating dairy cows. This thesis aimed to investigate milk cell population changes in lactating dairy cows in response to heat stress (HS) using a pair-feeding model (PFTN). …
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Autocrine mechanisms of action of insulin-like growth factor-I (IGF-I) and hormonal regulation of expression of IGF-finding proteins in mammary epithelial cells
… and IGF-binding proteins (IGFBPs) genes in mammary epithelial cells. To test the hypothesis that IGF-I affects growth of bovine mammary epithelial cells through an autocrine and/or paracrine pathway, several cell lines were developed expressing an ovine exon-2 containing IGF-I cDNA under the …
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Effects of Growth Hormone and Insulin-Like Growth Factor-I on Milk Protein Gene Expression and Nutrient Uptake and Cell Proliferation in Clonal Bovine Mammary Epithelial Cells
… and milk protein gene expression in bovine mammary epithelial cell line MAC-T cells were determined. GH increased (P < 0.01) expression of four major milk protein genes in MAC-T cells transfected with GHR expression plasmid. Cotransfection analyses indicated that GH also stimulated (P < …
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Modeling mammary epithelial cell polarization and the role of podocalyxin in breast tumor progression
The mammary gland consists of an organized network of epithelial ducts and lobules. This histoarchitecture can be recapitulated in vitro by culturing mammary epithelial cells as 3D spheroids embedded in a reconstituted basement membrane. I first used this assay to characterize the role of cell-cell …
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Extracellular Proteoglycan Decorin in Bovine Mammary Physiology
The majority of bovine mammary gland research focuses on the main cell types - mammary epithelial cells and fibroblasts. However, the extracellular matrix (ECM) within the mammary gland is also of importance for its ability to regulate cell shape, proliferation, polarity, differentiation, gene …
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Three-Dimensional Scaffolds for Mammary Epithelial Cell Growth
Mammary epithelial cells are highly efficient secreting cells. This natural phenomenon has triggered research efforts to focus on using these cells to assist in the production of viable liquids. The application of this technology is nearly endless. If developed successfully, mammary epithelial …
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A Proteomic Analysis of Differentiation in the Mammary Epithelium
… and the structural development of the mammary gland from pregnancy to lactation, very little is known about the molecular mechanisms governing differentiation of the mammary epithelium into a milk-secreting phenotype. It is important to acknowledge the diversity among the mammary glands …
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Microphysiometry Studies of Rapid Binding of Insulin-Like Growth Factor I by Parental and Transfected Mammary Epithelial Cell Lines
… uncontrolled proliferation of breast cancer cells. Insulin-like growth factor-I (IGF-I) is known to be a potent mitogen for mammary epithelial cells. IGF-I acts by binding to cell surface receptors, thereby stimulating a cascade of events leading to cell division. In the interest of …
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