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Showing 1 to 20 of 228 for “"Mammalian Cell"”.
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Viral Inhibition of Mammalian Cell DNA Synthesis
<p>Eukaryotic cells replicate their DNA during a defined time period in the interphase between mitoses. Non-proliferating cells are generally found to be blocked in interphase prior to the DNA-synthetic period. It therefore appears that the control of cellular proliferation may be intimately …
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Engineering mammalian cell line to improve sialylation
… of sialidase released to supernatant when the cell starts to die. This phenomenon is undesirable because the loss of terminal sialic acid results in a product which is rapidly removed from the plasma by the interaction with asialoglycoprotein receptors in the liver. Many studies have been done …
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Metabolic flux analysis in mammalian cell culture
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1993.
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Defining the contributors to mammalian cell mass
… many biosynthetic processes. To proliferate, a cell must not only physically divide but must also newly synthesize each of its components as it progresses through the cell cycle. Metabolism allows a cell to meet these demands. Metabolic alterations associated with proliferating cells have been …
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Molecular mechanisms of mammalian cell survival and differentiation
Cellular and developmental processes are regulated by extracellular and intracellular signals that are mediated by networks of signaling pathways. In recent years, microRNAs have also emerged as a class of critical modulators of the same processes. For my thesis studies, I focused on regulatory …
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Metabolic regulation of mammalian cell growth and proliferation
Proliferation requires that cells acquire sufficient biomass to produce two daughter cells. To accomplish this, cells must utilize available nutrients to generate new components of cell mass, including proteins, lipids, and nucleic acids. In addition, cells must coordinate biosynthesis and …
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Dynamic operation of mammalian cell fed-batch bioreactors
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Applied Biological Sciences, 1992.
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Dynamic organization of chromosomes in the mammalian cell nucleus
… the gene expression profile of an individual cell beyond the sequence information of the DNA. The present work focuses on the analysis of the dynamic aspects of higher order nuclear architecture in living cells. As a prerequisite, an in vivo replication labeling strategy was developed, that …
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The Role of Gene Expression Noise In Mammalian Cell Survival
… major challenge contributing to this problem is cellular heterogeneity. Even in the same environment, cells with identical genomes can display cell-to-cell differences in gene expression, also known as gene expression noise. Gene expression noise can vary in magnitude in a population or in …
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Metabolic flux analysis and population heterogeneity in mammalian cell culture
… analysis were used to develop relations between mammalian cell physiology and specific culture environments and to formulate strategies for increasing cell culture performance. Mitochondrial characteristics associated with respiration, membrane potential, and apoptosis along with physiological …
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Quantitative and mechanistic analysis of mammalian cell cultivation on microcarriers
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept of Nutrition and Food Science, 1984.
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Controlled Myogenic Differentiation of Mammalian Cell Lines by Temperature-Inducible Systems
… vitro generation of skeletal muscle tissue from cell lines is pivotal for the advancement of cultivated meat production. Here, we introduce a novel system employing a synthetic temperature-sensitive promoter (HSP70) carrying multiple heat shock elements (HSE), whose activity can be modulated by …
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Initiation and organization of the DNA replication in mammalian cell nuclei
Contains fulltext : mmubn000001_049070746.pdf (Publisher’s version ) (Open Access)
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Characterisation of a harvesting step for monoclonal antibodies from mammalian cell culture
… monoclonal antibodies secreted by GS-NSO cells: the filtration train blinded unpredictably and the ability of the positively charged filters to adsorb DNA from process material was unknown. To direct the development of an assay to quantify the ability of depth filters to adsorb DNA, the …
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Compatibility and toxicity of polymer-coated magnetic nanoparticles on mammalian cell systems
… do still exhibit some toxicity towards the cells, as the maximum cell density of cells cultured with particles does not reach that of control cultures. Both particles were found to increase the oxygen transfer in an aqueous solution. A 1.5% solution of particle A enhanced the oxygen transfer …
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Adherence to and invasion of mammalian cell lines by Pasteurella multocida B:2
… multocida have been reported to invade cultured mammalian cells, but the behaviour of other of serogroups has not been reported. The main object of the work was to confirm that HS strains of P. multocida B:2 have the capacity to invade and survive within cultured mammalian cells, such as J774.2 …
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IMPROVING GLYCOSYLATION EFFICIENCY IN ESCHERICHIA COLI BY MAMMALIAN-CELL-INSPIRED PROTEIN-PROTEIN INTERACTIONS
… called the oligosaccharyltransferase (OST). In mammalian cells, most proteins are N-glycosylated cotranslationally, mediated by protein-protein interactions between the OST and the general secretion (Sec) pathway. This mechanism is important for efficient glycosylation of many structured …
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Mammalian cell clonal growth and secretion measurements using gel microdroplets and flow cytometry
Thesis (Ph. D.)--Harvard University--Massachusetts Institute of Technology Division of Health Sciences and Technology, Program in Medical Engineering and Medical Physics, 1989.
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