Global ETD Search
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Showing 1 to 12 of 12 for “"Maltose-binding protein"”.
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Overexpression of Human Aryl Hydrocarbon Receptor in <i> E.coli</i> Using Two Different Solubility Enhancing Tags
… containing p23, Hsp90 and XAP2. Upon ligand binding, AhR undergoes conformational change and translocates into the nucleus. Once the AhR dimerizes with AhR nuclear translocator (Arnt), the chaperone proteins in the complex get dissociated followed by the activation of transcription of various …
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Crystallographic, Molecular Dynamics, And Enzymatic Studies Of Multi-Drug Resistant Hiv-1 Protease And Implications For Structure Based Drug Design (project 1); Crystallographic Studies Of Human Myelin Protein Zero (project 2)
… in A82F mutant show distorted S1/S1' binding pockets that causes loss of contacts and unstable binding of the inhibitors. Similarly, the mutants A82S and A82T show distortion in the S1/S1' binding pockets due to local changes in the electrostatics caused by the mutation from non-polar …
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Soluble and Functional Overexpression of the Ligand Binding Domain of Mouse Aryl Hydrocarbon Receptor in <i>E.Coli</i>
… and may elicit different responses upon ligand binding. The crystal structure of mouse AhR PAS-A domain was already obtained due to the robustness of mouse AhR in comparison to human. There is a possibility of overexpressing mouse AhR ligand binding domain in its soluble and functional form, …
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Investigation of a novel intein-based Escherichia coli expression system for human methylmalonyl CoA mutase : a thesis presented to Massey University in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry
… insoluble inclusion bodies, (ii) soluble fusion protein that cannot be separated efficiently from the fusion tag, or (iii) in low quantities. This research aimed to develop an E. coli expression system for the production of active human methylmalonyl CoA mutase, to enable x-ray crystallography …
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Molecular diversity and coat protein expression of Sweet potato leaf curl virus
… of a fragment of the replication-associated protein gene (AC1). Ten of these begomoviruses were closely related to SPLCV and one was closely related to Sweet potato leaf curl Georgia virus (SPLCGV). These results suggest that in the US, SPLCV may be more common in sweetpotato genotypes than …
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The development of transgenic plants resistant to cucumber mosaic virus and tobacco necrosis virus
… cDNA sequences of CMV-Wemmershoek (CMV-Wem) coat protein (CP) and TNV-F5P CP genes were cloned and subjected to sequence analysis. CMV-Wem is closely related to CMV-WL and CMV-Q, and therefore falls into CMV subgroup II. Similar analysis showed that TNV-F5P is closely related to TNV-A. By …
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Purification and Characterization of Native and Recombinant Dipeptidyl Aminopeptidase 1 of Plasmodium falciparum
… presents three challenges: 1) expression of the protein in soluble form, 2) generation of the native N-terminus, and 3) cleavage of the pro-domain. Soluble expression of DPAP1 was achieved by fusing it to the C-terminus of maltose-binding protein (MBP). A linker sequence encoding a tobacco etch …
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Expression of a tick toxin for the development of a canine vaccine
… of an anti-tick vaccine, Masina (1999) used a maltose-binding protein (MBP) holocyclus toxin (HT-1) fusion protein as immunogen. It was partially protective against challenges with crude tick extract in neonatal mice but unable to protect dogs against paralysis caused by direct tick attachment. …
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Analysis and Redesign of Protein-Protein Interactions: A Hotspot-Centric View
… discoveries from mutational analysis of protein interfaces is that often a large percentage of interface residues negligibly perturb the binding energy upon mutation, while residues in a few critical "hotspots" drastically reduce affinity when mutated. The organization of protein …
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Characterization of Novel Type VI Effectors of Acidovorax citrulli and Their Applicability to Biological Control of Plant Diseases
… A group of novel non-enzymatic effectors, Hyde1 proteins, delivered into prey cells through the T6SS, are responsible for this broad-spectrum antimicrobial activity. Expressing Hyde1 or its N-terminal transmembrane domain shows significant toxicity in both E. coli and AAC00-1, and the toxicity of …
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Production and characterization of the recombinant wheat chitinase Wch1 and generation of chitin-specific antibodies
… against wheat germ agglutinin (WGA) or chitin-binding domain (CBD). Five heptameric linear peptides were selected after showing specificity to the GlcNAc binding site on both WGA and D. stramonium lectin in an inhibition ELISA. All peptides were coupled to ovalbumin as the protein carrier and …