Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 54 for “"Magnetic beads"”.
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Magnetomicrometry : tissue length tracking via implanted magnetic beads
… a technology that tracks visually-obscured magnetic beads implanted within biological tissue to monitor in-vivo tissue length and speed within freely moving animals and humans. There are many methods to track visually-obscured objects, but magnetic-target tracking has the advantages of being …
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Quantum dot encoded magnetic beads for multiplexed fluorescence biosensing
In recent years, the use of encoded beads has received considerable attention due to their potential for measuring multiple analytes in solution.(1-4) This can be achieved without the need for knowledge of their spatial position, as in the case of microarray technology. Encoded bead technology also …
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Sparse Sample Detection Using Magnetic Bead Manipulation on a Digital Microfluidic Device
… sparse biological sample detection, which uses magnetic bead manipulation on a digital microfluidic device. Sparse sample detection was made possible through two steps: sparse sample capture and fluorescent signal detection. For the first step, the immunological reaction between antibody and …
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Synthesis and applications of functional magnetic polymer beads; synthesis and mass spectrometry analysis of model peptides
… the synthesis and application of functional magnetic polymer beads. The traditional suspension polymerization approach was used to synthesize polystyrene-iron oxide (Fe 3 O 4 ) based magnetic beads. The beads were coupled to different surface functional groups. The Fe 3 O 4 particles were …
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Magnetic bead-based DNA extraction and purification microfluidic chip
A magnetic bead-based DNA extraction and purification device has been designed to be used for extraction of the target DNA molecules from whole blood sample. Mixing and separation steps are performed using functionalised superparamagnetic beads suspended in the cell lysis buffer in a circular …
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Quantitative biological studies at cellular and sub-cellular level
We developed a translational magnetic tweezers for quantitative measurements at cellular and sub-cellular level. In the cellular studies, multiple membrane tethers were extracted simultaneously under constant force transduced to eukaryotic cells through magnetic beads attached to their membranes. …
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Imprinted Magnetic Traps for Study on Particle Fluctuation, Ordering and Microfluidic Applications
Superparamagnetic particles embedded in a polymer matrix (bead) have been a corner-stone to numerous interdisciplinary studies and applications across a wide range of fields such as physics, chemistry, material science and biomedicine. Through designed magnetic field profiles, these polymeric …
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Novel methods for on-site and cost-effective quantification of multiple targets using functional DNA and portable instruments
… a dipstick pre-treated with aptamer-conjugated magnetic beads, leading to the release of invertase-DNA strands. The strands then flows up with the liquid to an absorption pad pre-treated with sucrose, where the invertase linked on the strands converts sucrose into glucose in amounts proportional …
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Targeting the tight junction : immunotherapy of colon cancer
… novo protein engineering utilizing highly avid magnetic beads is described, in which extremely weak interactions can be captured from large library populations. Secondly, an in vitro assay which utilizes these highly avid magnetic beads is used to score the clinical immunogenicity of therapeutic …
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Studies of Enzyme Kinetics and Inhibition Through Capillary Electrophoretic Enzyme Assays
… of the dissertation focuses on the use of magnetic beads with immobilized enzymes for heterogeneous on-line enzyme assays. A study of bead surface chemistry effects on the formation and retention of magnetic bead plugs in a capillary during CE demonstrated that the type of biological …
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Detection of Progesterone in Cow’s Milk for the Prediction of Reproductive Performance
… detection. To reduce the milk matrix effect, magnetic beads conjugated with anti-P4 monoclonal antibodies (mAb) were used to separate P4 from milk. Subsequently, P4 concentrations were determined by surface plasmon resonance (SPR) inhibition assays. Initially, low binding was observed, and …
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Mechanically-induced intercellular remodeling of cardiomyocytes by magnetic micromanipulation
… gels and mechanical stimulation using magnetic micromanipulation. Cardiomyocytes were patterned on polyacrylamide gel using 25pm and 50pm microchannels. Mechanical stimulation was induced in sections with high densities of magnetic beads.
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A generic pathogen capture technology for sepsis diagnosis
… diagnosis. Our lab previously developed a micromagnetic-microfluidic pathogen isolation technology that can selectively remove pathogens from flowing whole human blood with high efficiency using micro- or nano-sized magnetic beads coated with microbe-specific antibodies [1, 2]. However, the …
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Conjugation of oligonucleotides for drug delivery via a linker derived from Meldrum’s acid and A capture-release method to synthesize long oligonucleotides
… the full-length sequences using azido-modified magnetic beads through a Copper-Catalyzed Azide–Alkyne Cycloaddition reaction. Subsequent ammonolysis reaction will release the unmodified full-length oligonucleotide sequences without the need for extensive purification.
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Developing a Novel Cell Surface RNA Detecting and Profiling Method via RNA Metabolic Labeling
… csRNA from total RNA, streptavidin-coated magnetic bead separation procedures could be used as a promising method for purifying csRNA from total RNA, for RNAseq characterization. This study highlights the processes of establishing the csRNA detection protocol and describes the current …
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Phenotypic and functional analysis of organ specific endothelial cells
… were isolated from murine livers using ɑ-CD146 magnetic beads and the phenotype compared to immortalised cell lines from heart (mUCEC-1), skin (s.END) and brain (b.End.5). The expression of a number of adhesion molecules and endothelial markers varied within cell type in response to …
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Quantitative analysis of subcellular biomechanics and mechanotransduction
… of cellular structures and receptor bound magnetic beads in response to applied strain or microscopic forces. These methods were applied to study the effects of several human disease associated mutations on subcellular mechanics and to examine the interaction between known protein function …
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Force-induced calcium concentration change and focal adhesion translocation : effects of force amplitude and frequency
… of force. Results from a while applying nN-level magnetic trap shear forces to the cell apex via integrin-linked magnetic beads. Both biological responses were monitored continuum, viscoelastic finite element model of magnetocytometry that represented experimental focal adhesion attachments …
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Surface Displayed SNAP as a New Reporter in Synthetic Biology
… cells to selectively bond to BG-conjugated magnetic beads. As a result, we have demonstrated that surface displayed SNAP facilitates engineering a direct channel between intracellular gene expression and extracellular material capture. In the near future, we believe this magnetic capture can …
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