Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Magnetic bead"”.
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Magnetic bead-based DNA extraction and purification microfluidic chip
A magnetic bead-based DNA extraction and purification device has been designed to be used for extraction of the target DNA molecules from whole blood sample. Mixing and separation steps are performed using functionalised superparamagnetic beads suspended in the cell lysis buffer in a circular …
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Sparse Sample Detection Using Magnetic Bead Manipulation on a Digital Microfluidic Device
… sparse biological sample detection, which uses magnetic bead manipulation on a digital microfluidic device. Sparse sample detection was made possible through two steps: sparse sample capture and fluorescent signal detection. For the first step, the immunological reaction between antibody and …
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Detection of Progesterone in Cow’s Milk for the Prediction of Reproductive Performance
… detection. To reduce the milk matrix effect, magnetic beads conjugated with anti-P4 monoclonal antibodies (mAb) were used to separate P4 from milk. Subsequently, P4 concentrations were determined by surface plasmon resonance (SPR) inhibition assays. Initially, low binding was observed, and …
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An experimental approach to determine the binding mode of yeast linker histone, Hho1p
… yeast linker histone Hholp to nucleosomes, using magnetic bead pull-down assays. To understand the structural and regulatory role of, Hholp, the linker histone in Saccharomyces cerevisiae, and that of higher eukaryotes (Thoma et al., 1979), it is essential to determine the manner in which these …
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Rapid Rac GTP-ASE activation in live cells by mechanical stress is independent of Src
… at the cellular and molecular level. Using the magnetic twisting cytometry technique, we applied local mechanical stresses to living human airway smooth muscle cells with a magnetic bead bound to the cell surface via transmembrane adhesion molecule integrins. The temporal and spatial activation …
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Modeling and control of a self-assembled robotic swimmer
… swimmer forms when a liquid suspension of ferro-magnetic micro-particles and a non-magnetic bead are exposed to an alternating magnetic field that is oriented per- pendicular to the liquid surface [1]. It can be steered by modulating the frequency of the alternating field. We model the swimmer as …
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Quantum dot encoded magnetic beads for multiplexed fluorescence biosensing
In recent years, the use of encoded beads has received considerable attention due to their potential for measuring multiple analytes in solution.(1-4) This can be achieved without the need for knowledge of their spatial position, as in the case of microarray technology. Encoded bead technology also …
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Development of Strategies for Improving the Isolation Efficiency and Protein Quantification of Extracellular Vesicles
… was performed. Firstly, the optimal magnetic bead sizes for efficient and rapid aptamer-based EV capture were determined. 1 and 2-3 μm aptamer functionalized magnetic beads provided complete and faster isolation of the EV-bead complex (EV bounds to the aptamer functionalized beads) …
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Measuring mechanical properties of the tectorial membrane with a microfabricated probe
… and are consistent with those obtained using the magnetic bead method [AF00].
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The effects of membrane and cytoskeletal mechanics on cell adhesiveness
… technology such as atomic force microscopy or magnetic bead microrheometry is necessary.
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Developing a Novel Cell Surface RNA Detecting and Profiling Method via RNA Metabolic Labeling
… csRNA from total RNA, streptavidin-coated magnetic bead separation procedures could be used as a promising method for purifying csRNA from total RNA, for RNAseq characterization. This study highlights the processes of establishing the csRNA detection protocol and describes the current …
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Neue Methoden der Proteinanalytik zur Identifikation von diagnostisch relevanten Molekülen für das Harnblasen- und Prostatakarzinom
… MALDI imaging). In particular, analysis of magnetic bead based fractioned serum samples of both entities resulted in sensitivity and specificity values above the standard values when comparing both entities among themselves as well as both entities with healthy controls. Tissue analysis of …
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Investigation and validation of mammalian telomeres as biomarkers for health and disease
… telomeric fragments, whereas column based and magnetic bead based methods produced significantly shorter TL estimates due to DNA fragmentation. Finally, novel methodological advances were established for chromosome-specific telomere sequencing using Oxford anopore Technologies, including 3′ …
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CRISPR Biosensors for Resource-limited Nucleic Acid Detection
… electrochemical sensing, and a field-deployable magnetic bead-based waterborne pathogen concentration and CRISPR-based detection system for environmental monitoring. This work also examines how local and indigenous knowledge figure into the conceptualization, collection, and utilization of …
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Magnetic microparticle trapping and mechanical excitation using domain walls in magnetic microstructures
… feasibility of using the resonant frequency of magnetic bead-domain wall (DW) couples in a host fluid to measure particle size. Nickel-Iron (Permalloy) rings, made using electron beam lithography, served as the tracks for nucleating and moving DWs, and Invitrogen Dynabeads M-270 magnetic beads …
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The generation and maintenance of T Helper 17 cells in response to bacterial infection
… Complex II (pMHCII) tetramer and magnetic bead-based enrichment method to study the differentiation of naïve, polyclonal, GAS or LM pMHCII-specific CD4+ cells into TH17 cells. We found that an intranasal route of infection resulted in TH17 differentiation, while an intravenous …
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Particle-enhanced biomolecule detection using silicon photonic microring resonators
… Chapter 3 details the initial developments of magnetic-bead based assays for lower limits of detection and extended dynamic range. Chapter 4 details the extension of previous magnetic particle detection by incorporating a permanent magnet into the chip holder in order to direct magnetic beads …
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Characterisation of Local Mechanical Properties in Living Tissues
… of tissues remains unknown. Four-pole electromagnetic tweezers have been developed to probe the mechanical properties of living embryonic tissues that are undergoing active morphogenetic development. The device is capable of generating magnetic forces in the order of nano-Newtons on a grafted …
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Role of the Neurotransmitter Glutamate in Neural Stem and Progenitor Cells Differentiation into Oligodendrocytes
… Enriched OPC cultures were purified using a magnetic bead sorting technique after which the cell lineage was confirmed using an immunocytochemistry (ICC) staining technique. Results demonstrated that cells differentiated alone with no external stimulation did not produce the myelin basic …
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Studies of Enzyme Kinetics and Inhibition Through Capillary Electrophoretic Enzyme Assays
… of the dissertation focuses on the use of magnetic beads with immobilized enzymes for heterogeneous on-line enzyme assays. A study of bead surface chemistry effects on the formation and retention of magnetic bead plugs in a capillary during CE demonstrated that the type of biological …
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