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Showing 1 to 20 of 39 for “"Magic-angle spinning (MAS)"”.
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Solid state nuclear magnetic resonance methodology for biomolecular structure determination
… are presented. All studies are performed with magic angle spinning (MAS) and high-power proton decoupling, for optimal sensitivity and resolution. Chemical shift are assigned by multi-dimensional correlation spectroscopy in isotopically enriched molecules ...
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Multinuclear Solid-State NMR Studies of Supported Transition Metal Carbonyls
… and oxide-supported metal carbonyl complexes. Magic-angle spinning (MAS) C-13 NMR spectra of polycrystalline metal carbonyl clusters yield both isotropic chemical shifts and chemical shift anisotropies (CSA's) for the carbonyl ligands, providing information on solid-state symmetry, J-coupling, …
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Investigation of structural phase transitions in minerals and analogue systems by high-temperature magic-angle-spinning nuclear magnetic resonance spectroscopy
… magnetic resonance spectroscopy using mostly magic-angle spinning (MAS) techniques. The results provide important insight into each of these SPT's and illustrate the types of information that can be obtained about SPT's using MAS NMR spectroscopy.
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Proton-coupled carbon-13 sample spinning NMR studies of macromolecules and ordered systems
… (NMR) spectroscopy. We find that under ""magic angle"" spinning (MAS) these systems display high resolution due to extensive motions, and therefore traditional proton-decoupling is not necessary. In the absence of proton decoupling, i.e., proton-coupled MAS (PCMAS), we find that these …
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Insights into structure, dynamics of thioredoxin reassembly and dynein light chain 8 and ionization states of thiamin cofactor by MAS NMR spectroscopy
Magic angle spinning (MAS) NMR spectroscopy has found applications as a versatile technique in chemistry and biochemistry to probe structure and dynamics of materials and biological systems. In recent years, MAS NMR spectroscopy has been rapidly developing as a method for atomic-resolution …
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Applications of DNP and solid-state NMR for protein structure determination
Magic Angle Spinning (MAS) solid state nuclear magnetic resonance (SSNMR) is a developing method for determining the structures and studying the dynamics and functions of biological molecules. This method is particularly important for systems, such as amyloidogenic fibrous proteins, that do not …
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Solid-State 17O NMR as a New Probe to Study Acyl-Enzyme Intermediates
… solid-state 17O NMR spectra obtained under the magic angle spinning (MAS) condition at 21 T for the three acyl-enzyme intermediates yields the following isotropic chemical shift (δiso), quadrupolar coupling constant (CQ), and asymmetry parameter (ηQ): DAB-CHT, δiso = 316 ± 2 ppm, CQ = 10.0 ± 1 …
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Structure and function of the Influenza membrane protein M2 by magic angle spinning NMR and dynamic nuclear polarization
… structural characterization of proteins using magic angle spinning (MAS) nuclear magnetic resonance (NMR) applied to the membrane protein M2 from Influenza A, a small helical transmembrane proton transporter that is the target of adamantane based inhibitors but which is now drug resistant due …
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Solid-State Magic-Angle Spinning NMR Methods for Tensor Measurements and Protein Structure Refinement Using Chemical Shift Tensors
… (CSA) pseudostatic powder lineshapes, slow magic angle spinning (MAS) analysis of highly-13C,15N-enriched solid proteins using 2D heteronuclear correlation at 750 MHz 1H frequencies, rotational resonance (R2) lineshape analysis, and distance information using the REDOR and TEDOR …
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Molecular and spin dynamics in solid state nuclear magnetic resonance spectroscopy
… interference between molecular dynamics and Magic Angle Spinning (MAS) is examined. Agostic interactions had been hypothesized to exist in both compounds, but the unusual molecular dynamics are explainable without invoking a relation to such interactions. Further, no evidence is found to …
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Sonically induced narrowing of the NMR spectra of solids and other novel NMR techniques
… polarising magnetic field, as implemented in the magic-angle spinning (MAS) [1], dynamic angle spinning (DAS) [2] and double rotation (DOR) [3] NMR experiments. Recently, an alternative method, sonically induced narrowing of the NMR spectra of solids (SINNMR) [4], has been reported which yields …
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High Field DNP NMR probe design and application in crystalline solids
… radio frequency NMR circuit for a triple channel magic angle spinning (MAS) DNP NMR probe for operation at 5 Tesla are developed, and the construction of the probe is detailed. The probe carries out NMR in three frequency ranges, corresponding to the 1H, 1 3C, and 15N Larmor frequencies at 5 T, …
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Amyloid fibril structure of peptides and proteins by magic angle spinning NMR spectroscopy and dynamic nuclear polarization
… to form amyloid fibrils in vitro. We have used magic angle spinning (MAS) NMR spectroscopy to investigate the structure of two amyloid fibril systems - an 11- residue segment from the disease-related protein transthyretin (TTR); and P2- microglobulin (32m), a 99-residue protein associated with …
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Advances in Instrumentation for Dynamic Nuclear Polarization & Magic-Angle Spinning NMR
… tool for increasing the sensitivity of magic angle spinning (MAS) nuclear magnetic resonance (NMR) experiments. Historically, one of the primary drawbacks of DNP has been limited spectral resolution. The resolution of DNP spectra can be substantially improved via data acquisition at …
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High field DNP and cryogenic MAS NMR : novel instrumentation and applications
… involved completion of the world highest field magic angle spinning (MAS) DNP spectrometer and a probe for 16.4T, initial DNP experiments on ¹⁷ O nuclei, variable temperature studies of a model tripeptide, and a systematic analysis of a novel approach to high efficiency RF circuit design. The …
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Solution and solid state NMR studies of fluorine tagging reagents
… solid-state NMR with cross-polarization (CP), magic angle spinning (MAS), and high-power proton decoupling. The ¹⁹F dipolar coupling interactions, created by the presence of the fluorine tag, attenuate signals for these nuclei in the immediate proximity of the tagged site. A series of …
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Solid State NMR Relaxation Studies of Triosephosphate Isomerase
… strengths were utilized. R1ρ experiments and magic angle spinning (MAS) one-dimensional spectra were used to characterize phosphate ligand binding in the glycolytic protein triosephosphate isomerase. 1D spectra indicated the presence of both isotropic and anisotropic phosphate populations. …
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Nuclear magnetic resonance investigation of mineral surface reactions involving inner-sphere and outer-sphere complexes
… surfaces, using $\sp{31}$P and $\sp{133}$Cs magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy. Phosphate and Cs are good examples of inner-sphere and outer-sphere complexes, respectively, and this study shows that NMR is in many ways a more useful tool to investigate …
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New Tools for Structural Biology and Biophysics: High-Throughput Fluorine Solid-State NMR and Applications to Membrane Proteins
… new SSNMR methods are developed, utilizing fast Magic Angle Spinning (MAS), multidimensional correlation, and the 19F nucleus as a biophysical probe for the understanding the structure and dynamics of crystalline and membrane-bound proteins and protein-ligand complexes. Internuclear distances are …
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Applications of high resolution solid state carbon-13 NMR to the study of multicomponent polymer systems
… solid state carbon-13 NMR, achieved with magic angle spinning (MAS) and high power 13c-1H dipolar decoupling and cross polarization, was applied to a number of multicomponent polymer systems. Specific problems which were addressed are: (1) the nature of domain structure and molecular …
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