Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Mad2"”.
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Regulation of the spindle checkpoint by Mad2 binding proteins
… Checkpoint signaling requires binding of Mad2 to the checkpoint protein Madl and Cdc20, a subunit of the Anaphase Promoting Complex. We have characterized the interactions of human Mad2 with Madl, Cdc20, and CMT2, a checkpoint inhibitor. Cdc20 and Madl form competitive high affinity …
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Histone H3 K4 Methylation Regulates The Spindle Assembly Checkpoint Through Direct Binding of Multiple Checkpoint Components and Cdc20
… to Spindle Assembly Checkpoint proteins Bub3 and Mad2 as well as the activator of the Anaphase Promoting Complex (APC) protein Cdc20. <em>MAD2</em> encodes a HORMA domain, which I identify as a novel histone binding motif. Mad2 maintains two differing conformations, closed Mad2 (C-Mad2) and open …
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Identification and characterization of Sgo2 interactions - Insights into dynamic chromosomal localization, mechanism of cohesin protection and putative checkpoint function
… Central to SAC signaling, kinetochore bound Mad1-Mad2 complex catalyzes a conformational switch of soluble Mad2, thereby allowing its inhibitory binding to the downstream effector protein Cdc20. SAC-dependent inhibition of anaphase correlates well in timing with shugoshin-dependent protection of …
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Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
… securin. The MCC is composed of BubR1, Cdc20 and Mad2, and while their assembly is an intrinsically very slow process, in cells it is catalytically activated. Recent work points towards hierarchical recruitments of SAC proteins onto the outer kinetochore by means of a Mps1-dependent …
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ELUCIDATION OF THE ROLE OF FAT10 IN TUMORIGENESIS
… hepatocytes. This thesis also highlighted Mad2-binding as an important mechanism by which FAT10 exerts its effect on cell malignancy. Therefore, based on our collective data, we have demonstrated that FAT10 exerts its pro-malignancy effects through disruption of mitotic spindle checkpoint …
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TO INVESTIGATE THE ROLE OF RNF8 IN REGULATING MITOTIC PROGRESSION IN GLIOMAS
… proteomics, we find that RNF8 interacts with MAD2 and CAMK2D via its RING and FHA domains respectively to activate the SAC response. The physiological relevance of our mechanistic findings is illustrated in GSCs whereby the overexpression of wild-type RNF8, but not the FHA or RING mutant, …
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Safeguard of Mitosis: The Spindle Checkpoint
… complex (MCC), which consists of BubR1-Bub3, Mad2, and Cdc20. The diffusible MCC inhibits the ubiquitin ligase activity of the anaphase-promoting complex or cyclosome bound to its co-activator Cdc20 (APC/C-Cdc20) to arrest cells in mitosis. Nevertheless, it remains unknown how the checkpoint …
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The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint
… sequestration of Cdc20 by the SAC components Mad2 and BubR1. The consequent inactivation of the APC/C causes a metaphase arrest and gives the cell time to correct the error. Given its great importance for chromosome segregation fidelity, it comes at no surprise that loss of the SAC causes cell …
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Characterisation of the KA1 & KA2 domains and interaction with APC/C
… band of the APC/C complex which was shown to be Mad2, an important subunit of the APC/C inhibitor complex the mitotic checkpoint complex (MCC). These finding suggest a new role for KA contain kinases as regulators of APC/C activity. Future studies to identify the residues of Mad2 which are …
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Role of retinoblastoma protein in the development of liver cancer
… of the G2/M checkpoint proteins such as Gadd45a, MAD2 and CDC25a all show a dysregulation in their activities. In conclusion Rb loss leads to aberrant regulation of p53 and induces DNA damage, this superimposed on a background of a lack of cell cycle control predisposes the hepatocyte to becoming …
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Investigation of the role of chromosome missegregation in embryo development and in tumorigenesis
… 3 will detail the creation and analysis of a Mad2-/- mouse embryonic fibroblast cell line that is viable due to a simultaneous disruption in p53.
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The role of ZW10 and its binding partners in chromosome segregation
… of previously proposed downstream player, Mad2. In addition, I identified a ZW10's interaction with BubRI that may suggest a possible role for ZW 10 in the checkpoint signaling pathway. Thus RZZ complex acts at the interphase of attachment and signaling at kinetochores suggesting a close …
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Changes in Kinetochore Structure and Molecular Composition in Response to Mis-attachment
… thanks to the accumulation of the SAC protein Mad2 at the KT. Our data also indicate that structural changes of the KT, measured as intra- or inter-KT stretching, do not directly induce a SAC response. Instead, our findings suggest decreased KT stretching, especially in inter-KT stretching of …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… of APC in vitro,much more efficiently than Mad2. Surprisingly, the kinase activity of BubR1 or association with Bub3 is not required for the inhibition of APCCdc20. Furthermore, BubR1 restores the mitotic arrest in Cdc20-overexpressing cells treated with nocodazole. Mps1 is a dual …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… of APC in vitro,much more efficiently than Mad2. Surprisingly, the kinase activity of BubR1 or association with Bub3 is not required for the inhibition of APCCdc20. Furthermore, BubR1 restores the mitotic arrest in Cdc20-overexpressing cells treated with nocodazole. Mps1 is a dual …
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Regulation of tubulin functions in Saccharomyces cerevisiae
… of tubl-729 cells in which deletion of MAD2 has eliminated the spindle defect checkpoint. This suggests that Bub3p does not act through the spindle defect checkpoint to suppress tubl-729 cells. We present preliminary evidence in support of a model proposing that tubl-729 cells are …
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Role of phosphatases in the end-on conversion process
… spindles and prolong mitosis time with increased Mad2 levels at the outer-kinetochore. A kinase inhibitor assay showed that kinetochore-microtubule attachment defects in Astrin mutant expressing cells could be rescued when both Aurora B and Cdk1 kinases are inhibited, suggesting a role for …
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Mitotic slippage by ATP depletion and its mechanism
… assembly checkpoint proteins including BubR1, Mad2, and MCAK. However, the localization and the kinase activity of Aurora B, which is widely recognized as a component of error correction of microtubule attachment and its role in maintenance of spindle assembly checkpoint, were reduced on …
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Role of Ame1, a central component of the Saccharomyces cerevisiae kinetochore, in kinetochore function and structure
… of the spindle assembly checkpoint by Mad2 depletion also leads to only a partial rescue of the spindle defect. iv) A considerable number of cells separate their spindle pole bodies in presence of active Esp1, but nevertheless display a spindle defect. v) Most spindle defects occur at …