Global ETD Search
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Showing 1 to 10 of 10 for “"Mad1"”.
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The Multifunctional Kinase Bub1 Acts as a Signaling Hub for the Spindle Checkpoint
… other important checkpoint proteins like BubR1, Mad1 and Cdc20. We found phosphorylation on Bub1 serine 459 is essential for spindle checkpoint and for Bub1-Mad1 interaction. However, the majority of Mad1 still localize to the kinetochore in cells expressing Bub1-S459A mutant. These results …
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Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
… explore the structure and function of the Bub1- Mad1 complex, including how sequential phosphorylation of the Bub1 CD1 domain by Cdk1 and Mps1 promotes kinetochore targeting of the Mad1:C-Mad2 complex. Chapter 4 investigates how Mad1 C-terminal phosphorylation by Mps1 promotes juxtaposition of …
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Examining the Regulation of Cell Division by the Spindle Assembly Checkpoint
… the localization of the checkpoint protein Mad1 and show that its constitutive presence at the kinetochore is sufficient to induce checkpoint arrest. Being able to uncouple checkpoint signaling from chromosome biorientation, I use the system as a tool to examine the roles of checkpoint …
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Safeguard of Mitosis: The Spindle Checkpoint
… Bub1 and mitosis arrest deficiency 1 (Mad1). Phosphorylated Bub1 (pBub1) binds with Mad1-Mad2. Phosphorylated Mad1 (pMad1) directly interacts with Cdc20. Mutations of Mps1 phosphorylation sites in Bub1 or Mad1 abrogate the spindle checkpoint in human cells. Therefore, Mps1 promotes …
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Identification and characterization of Sgo2 interactions - Insights into dynamic chromosomal localization, mechanism of cohesin protection and putative checkpoint function
… Central to SAC signaling, kinetochore bound Mad1-Mad2 complex catalyzes a conformational switch of soluble Mad2, thereby allowing its inhibitory binding to the downstream effector protein Cdc20. SAC-dependent inhibition of anaphase correlates well in timing with shugoshin-dependent protection …
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Functional analyses of microtubule and centrosome-associated proteins in Dictyostelium discoideum
… the putative spindle assembly checkpoint protein Mad1. While a GFP-CP103 fusion protein could clearly be localized to isolated centrosomes that are free of microtubules, Cenp68 and Mad1 were found to associate with the centromeres and kinetochores, respectively. The investigation of Cenp68 …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… fractured spindles. In the absence of hSpc25, Mad1 and Hec1 fail to localize to kinetochores during mitosis whereas the kinetochore localization of Bub1 and BubR1 is largely unaffected. Interestingly, the kinetochore localization of Mad1 in cells with a compromised Ndc80 function is restored …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… fractured spindles. In the absence of hSpc25, Mad1 and Hec1 fail to localize to kinetochores during mitosis whereas the kinetochore localization of Bub1 and BubR1 is largely unaffected. Interestingly, the kinetochore localization of Mad1 in cells with a compromised Ndc80 function is restored …
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Regulation von Teilungswahrscheinlichkeit und Zellzyklusdauer durch das Onkoprotein c-Myc
… wirkenden Transkriptionsfaktors Mad1 durch Zugabe von Tetrazyklin steuern. Der Antagonist Mad1 senkte vor allem die Teilungswahrscheinlichkeit der Zellen. Zusammenfassend ergibt sich daraus der Vorschlag eines neuen Modells. Die Expression von c-Myc in einer Zelle beeinflusst vor …
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The effect of civil unrest on nursing personnel and nursing services in the Cape Peninsula during 1986
… equipped to cope with the changing demands mad1:1 upon them during periods of political instability; that civil unrest negatively affects nursing personnel and nursing services; and further, that disruption of a service impacted other services. It is recommended that in areas of violence it …