Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 32 for “"Macropinocytosis"”.
-
Therapeutic targeting of DGKA-mediated macropinocytosis in lymphangioleiomyomatosis
… pathways of extracellular nutrient acquisition. Macropinocytosis, a form of clathrin-independent endocytosis, is upregulated in TSC2-deficient cells. We performed a high-throughput compound screen utilizing a repurposing drug library. We identified that ritanserin, a diacylglycerol kinase alpha …
-
Selective transfer of exosomes from oligodendrocytes to microglia by macropinocytosis
Selektiver Transfer von Exosomen von Oligodendrozyten zu Mikroglia durch Makropinozytose Oligodendrozyten sind die Myelin-bildenden Zellen des zentralen Nervensystems. Dieser Zelltyp bildet während der aktiven Phase der Myelinisierung große Mengen an Zellmembran aus. Zusätzlich werden …
-
Dock180/Elmo proteins are critical regulators of phagocytosis,macropinocytosis and chemotaxis in Dictyostelium
… and describe their role in phagocytosis, macropinocytosis and CAMP mediated chemotactic cell movement. Aggregation competent Dictyostelium cells can detect and move up CAMP gradients. cAMP stimulation causes a transient localized increase in phosphatidyl inositol (3,4,5) tris phosphate …
-
A Novel Syndecan-1 Targeted Therapeutic Antibody Inhibits Macropinocytosis and Induces Anti-Tumor Immunity in Pancreatic Cancer
… which promotes cancer progression by mediating macropinocytosis. Moreover, SDC1 plays an essential role for the acquired resistance to KRAS* inhibition in PDAC, making it an attractive new therapeutic target, as allele-specific and pan-KRAS* inhibitors continue progressing through pre-clinical …
-
Regulation of Fluid-phase Uptake in Podocytes by Albumin-associated Lipids
… lipids associated with serum albumin stimulate macropinocytosis in podocytes. This process was specific to podocytes as known stimuli that induce macropinocytosis in other cells had no effect on podocytes, while serum lipids did not stimulate macropinocytosis in other cells. A candidate lipid …
-
Macrophage Activation Profile and Phenotype Are Regulated by Metabolic Pathways and Are Altered by Diabetic Conditions
… by an energy dependent process called macropinocytosis. Chapter four provides evidence that macropinocytosis is decreased in peritoneal macrophages from diabetic mice. Since diabetes is characterized by metabolic abnormalities and altered immune function we tested the effect of …
-
The Molecular and Cellular Mechanisms for Nanoparticle Transport
… that tumour endothelial cells predominantly use macropinocytosis to transport nanoparticles into the tumour. Macropinocytosis membrane ruffling is upregulated in the tumour endothelium compared to normal endothelium in healthy tissues, which might explain the elevated nanoparticle accumulation in …
-
TRPML1 Promotes Protein Homeostasis in Melanoma Cells by Negatively Regulating MAPK and mTORC1 Signaling
… survival, proliferation, tumor growth, and macropinocytosis as well as serine depletion and proteotoxic stress. All of these phenotypes were partially or completely rescued by mTORC1 inhibition. Melanoma cells thus increase TRPML1 expression relative to melanocytes to attenuate MAPK and …
-
The Function of CARMIL1 in Migrating Cells
… ruffling at the leading edge of motile cells and macropinocytosis were also found to be dependent on the CARMIL1-CP interaction. This is consistent with macropinocytosis and ruffling being dependent on a functional and dynamic lamellipodium.: Kerr and Teasdale, 2009).</p><p>In chapter two I …
-
Mechanisms of Coral Calcification: a Geochemical and Computational Approach to Understanding Biomineralisation
… that involves a recently proposed process, ‘macropinocytosis’, playing a key role in coral biomineralisation. To test the importance of the macropinocytosis mechanism, I developed a novel dynamic model of calcifying fluid chemistry - the first to explicitly integrate macropinocytosis. This …
-
Analysis of Cellular Factors Involved in Adeno-Associated Virus Type 2 Entry
… caveolar-independent. We show that inhibitors of macropinocytosis, which did not inhibit the clathrin-dependent entry pathways, blocked the entry of AAV and HSPG as well as blocked AAV transduction. In cells that are deficient in cell surface HSPG, transduction by AAV was reduced but unaffected by …
-
Sphingomyelinases and Lysosomal Trafficking in Ebolavirus Entry
… entry mechanisms. EBOV enters the cell using a macropinocytosis-like mechanism, which requires actin, microtubules, and phosphatidylinositol 3-kinase (PI3K). Additionally, pH-dependent lysosomal enzymes, cathepsins B and L, are needed to proteolytically cleave the virus surface glycoprotein. …
-
Neutrophil biomechanical properties and immune function in health and inflammatory disease
… Neutrophil immunologic functions (e.g. NETosis, macropinocytosis) were imaged using florescence microscopy. To analyse the contribution of biomechanical properties to neutrophil trafficking, a novel microfluidic microvasculature mimetic was developed. An endothelial flow assay was used to image …
-
Regulatory role of phosphatidylinositol 4-phosphate 5-kinases in brain inflammation
… cell survival, cell proliferation, phagocytosis, macropinocytosis membraned ruffles, ion channels transporters, cytoskeletal regulation and intracellular vesicle trafficking. Recently, it was reported that PI(4,5)P2 was important for neurodegenerative diseases. Nevertheless, expression and …
-
Domain-based Bioinformatics Analysis and Molecular Insights for the Autoregulatory Mechanism of Phafin2
… autophagy, endosomal cargo transportation, and macropinocytosis. Two domains, namely, PH and FYVE, contribute to Phafin2's cell membrane binding. Phafin2 also contains a poly aspartic acid (polyD) motif in its C-terminal region that can specifically autoinhibit the PH domain binding to membrane …
-
Nanoscale Organization of The Small Gtpase Rac1
… of the protein and resulted in impaired macropinocytosis and decreased cell spreading. In this thesis, I proposed that Rac1 nanoclusters act as lipid-based signaling platforms emulating the spatiotemporal organization of Ras proteins and further showed that the Rac1 PBD-prenyl anchor has …
-
Enhancement Of Ebola Virus Infection By Seminal Amyloid Fibrils
… then subsequent internalization of virus through macropinocytosis, a previously unknown ability to enhance uptake by this mechanism critical for Ebola virus entry. Finally, a new ability of SEVI to enhance viral stability during incubation at physiologic temperatures and under desiccating …
-
The Interplay of Signal Transduction and Membrane Traffic of the Macrophage Colony Stimulating Factor Receptor (MCSFR) Controls Macrophage Growth
… and degraded. Pharmacological disruption of macropinocytosis impairs receptor degradation. Entry into endocytic pathway is mediated by the covalent addition of the protein ubiquitin to the MCSFR by the ubiquitin-ligases Cbl and Cbl-b in the hematopoietic stem cell (HSC) compartment. These …
-
Colony Stimulating Factor-1 Receptor (CSF-1R) Expression, Degradation, and Signaling in Lipid-Laden Macrophages
… did not display differences in CSF-1 stimulated macropinocytosis as observed with live-cell microscopy. Flow cytometry analysis revealed lipid laden macrophages had an increase in CD68, with decreased expression of CD11B and CD80. Differences in activation of signaling molecules highlight …
-
Heparan Sulfate Proteoglycans in Pancreatic Ductal Adenocarcinoma (PDAC)
… localization on the cell surface and sustain macropinocytosis, enhance the activation of multiple RTKs, thereby promoting resistance to KRAS*-targeted therapy. Overall, our study has provided the rationale for therapeutic targeting of the YAP-SDC1 axis to overcome resistance to KRAS* …
Page 1 of 2