Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Macroautophagy"”.
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Investigation of mTOR-independent regulation of macroautophagy
Investigation of mTOR-independent regulation of macroautophagy Richard Ian Odle Macroautophagy is a critical catabolic response to cellular stress, enabling lysosomal mediated breakdown of cytosolic cargo. The nutrient-responsive mTORC1 kinase complex has been described as a master regulator of …
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Polar Body Membrane Breakdown Occurs Independent of Macroautophagy in C. Elegans
… roles in regulating LC3 recruitment during macroautophagy and LAP. We demonstrated that the ATG16L1 <em>C. elegans</em> orthologs, ATG-16.1 and ATG-16.2, function redundantly to promote polar body membrane breakdown. We also discovered that truncating the LAP-specific WD40 domain of ATG-16.2 …
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The Effects of a Purified B-Group Soyasaponin Fraction on the Proliferation, Cell Cycle Progression and Induction of Macroautophagy in Hct-15 Human Colon Adenocarcinoma Cells
Colon carcinoma is the second largest cause of cancer-related mortality in the United States. Diet has been recognized as an important modifier of colon cancer development for decades. Specifically, dietary patterns that include legumes, such as soybeans, may reduce colon cancer progression. High …
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Effects of mitochondrial dysfunction on neurofilament turnover and distribution in human neuroblastoma cells
… revealed that NF-heavy chains are degraded by macroautophagy and cathepsin D, possibly with some involvement of cysteine cathepsin, but not calpain or the ubiquitin proteasome system (UPS). This is in contrast to α-synuclein which was degraded by macroautophagy, the UPS and calpain. Treatment …
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A role for HSC70 in regulating antigen trafficking and presentation during macronutrient deprivation
… exploit several pathways including endocytosis, macroautophagy, and chaperone-mediated autophagy to capture and deliver antigens to the endosomal network. Within the endosomal network antigens are processed and loaded onto major histocompatibility complex (MHC) class II molecules for display and …
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Molecular analysis of vesicle biogenesis during autophagy
… into the vacuolar lumen for degradation. During macroautophagy out of the pre- autophagosomal structure (PAS) autophagosomes are formed that fuse with the vacuole for degradation. During microautophagy cargo is directly engulfed by the vacuolar membrane. One aim of this study was to identify …
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Autophagy gridlock in tauopathy
… which was correlated with a slight increase in macroautophagy induction. The autophagic intermediates were immunoreactive for Blue Cheese, the fly homolog of autophagy-linked FYVE protein, ALFY. A blockage in autophagic flux also contributed to the accumulation of autophagic intermediates with …
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Tau aggregation is heterogeneous across cases of sporadic Alzheimer's disease and is influenced by autophagy pathways in vitro
… that inhibition of intracellular pathways of macroautophagy and endosomal microautopahgy heterogeneously increased tau aggregation and changed tau aggregation kinetics, dependent upon the case of Alzheimer's disease. These results inform that tau aggregates vary significantly not just between …
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Fluorescence lifetime imaging microscopy to study protein aggregation in the context of neurodegenerative diseases
… reticulum (ER) and mitochondrial fission. Macroautophagy needed for degradation and clearance of aberrant FUS aggregates is inhibited despite an up-regulation of TFEB and increased lysosomal biogenesis. Although the sequence of events leading to macroautophagy inhibition which perpetuates …
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Characterising Futile Autophagosome Based Toxicity and its Implications in Disease
Macroautophagy (‘autophagy’ hereafter) mediates the capture of aberrant cytoplasmic material into vesicles called autophagosomes, which then shuttle to lysosomes for degradation. Autophagy is implicated in numerous diseases, largely in a pro-survival role. However, autophagy has also been suggested …
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Isolation and characterization of Saccharomyces cerevisiae mutants for vacuolar import and autophagocytosis
… involves proteins that are involved also in macroautophagy. The cytosolic precursor protein and the matured vauolar protein from homododecameric complexes. Only the matured homododecameric complex shows enzymatic activity. We developed a new genetic screen to isolate mutants in the biogenesis …
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The SYK tyrosine kinase suppresses autolysosome biogenesis via activation of mTORC1 in pancreatic cancer cell lines
… complex suppresses lysosome biogenesis and macroautophagy (autophagy). Consequently, mTORC1 suppression via SYK inhibition or shRNA-mediated depletion causes accumulation of autolysosomes. These effects are mediated by the enhanced nuclear localization of MITF, a key transcriptional …
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Investigating the role of ULK1 kinase activity during autophagy
Macroautophagy (hereby autophagy) is a catabolic pathway whereby a double membrane organelle called an autophagosome, engulfs cytoplasmic components in order to degrade them via the lysosome. While autophagy is induced in response to various stresses to promote homeostasis and cell survival, a key …
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Investigating how mitophagy is regulated by the metabolic context
The selective degradation of mitochondria via macroautophagy, termed mitophagy, is a key quality control mechanism for mitochondrial network homeostasis, cell integrity and organism development. In mice, basal mitophagy is pervasive across mouse tissues, being particularly high in tissues of high …
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Modelling neurodegenerative diseases in human iPSC-derived neurons
… ATG8 gene family, which are core components of macroautophagy. This work exemplified both the strengths and weaknesses of iPSC-derived neurons; namely, they are tractable and able to recapitulate neural phenotypes, but deriving new model lines requires a high initial investment, so adequate …
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Investigating alterations in autophagy in Alzheimer’s disease using human brain tissue and skin-derived fibroblasts
… dysfunction of the two main autophagy pathways-macroautophagy (MA) and chaperone-mediated autophagy (CMA) - and AD. However, it is unclear how the autophagic pathway activity changes during disease progression and how this relates to the accumulation of abnormal protein deposits in the brain. …
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The role of DAXX in the selective autophagy receptor SQSTM1/p62 phase condensation
Macroautophagy (‘autophagy’ hereafter) is a lysosome-dependent degradation system for intracellular protein quality control. Autophagy can selectively remove cargo materials, which is dependent on cargo receptors. SQSTM1/p62 (‘p62’ hereafter) is one of the best characterised receptors mediating the …
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Structural Investigations of HIV-1 Restriction: Roles of Autophagy in TRIM5α and HIV-1 Capsid in Complex with a Novel Inhibitor
… remains elusive. One hypothesis includes macroautophagy, a cellular bulk-degradation pathway with known implications in host-pathogen interactions. TRIM5α contains a novel α-helical LC3-interacting motif, which mediates the interaction between it and the mATG8 family of proteins. The mATG8 …
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Scalable methods for the discovery of autophagy and disease genes
Macroautophagy (henceforth referred to as autophagy) is a major and conserved cellular process in which cells deliver cytoplasmic contents to lysosomes for degradation. As autophagy has been linked to various human diseases, the discovery and characterisation of autophagy genes is of interest for …
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The role of CASM in the endolysosomal damage response
… integral roles in degradative processes such as macroautophagy and phagocytosis, in addition to engaging in other key cellular processes such as antigen presentation. Endolysosomal (hereafter referred to as lysosomal) damage can occur in response to various cell intrinsic and extrinsic factors, …
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