Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 30 for “"MYCN"”.
-
Arid1a Haploinsufficiency Initiates Neural Crest Transformation in a Mouse Model of Mycn-driven Neuroblastoma
… ARID1A’s candidacy as a putative 1p36 TSG in MYCN-driven NBL. This study aimed to causally test Arid1a loss during Mycn-driven NBL initiation through the development of a mouse model of high risk NBL.In this study, we modified a Mycn-driven mouse model of NBL to incorporate Cre mediated …
-
Inibizione selettiva del gene MYCN mediante PNA (acidi peptido nucleici) anti-gene nel rabdomiosarcoma umano
MYCN oncogene amplification/expression is a feature of many childhood tumors, and some adult tumors, and it is associated with poor prognosis. While MYC expression is ubiquitary, MYCN has a restricted expression after birth and it is an ideal target for an effective therapy. PNAs belong to the …
-
Morphological classification of childhood medulloblastomas with β-catenin immunohistochemistry and mycn fluorescent in situ hybridization
… describe the expression status of β-catenin, and MYCN, using IHC and FISH respectively, and to correlate these findings with clinico-pathological and demographic characteristics and clinical outcome. Materials and Methods This study was a nested retrospective analytical study, reviewing 54 cases …
-
Chromosome dynamics and genomic instablity in neuroblastoma. Three genomic pillars: MYCN amplification, numerical and structural changes.
… neuroblastoma -- amplification of the oncogene MYCN in the tumour cells. Because amplified MYCN typically reside in ring-formed chromatin structures lacking centromeres (so-called double minutes, DMs) it remained unknown for a long time how the amplified sequences were maintained in the growing …
-
Integrative analysis of the metastatic neuroblastoma transcriptome
… Viral Oncogene Neuroblastoma Derived Homolog (MYCN) amplified NBLs showed a distinct splicing pattern affecting multiple cancer hallmarks. Six splicing factors have altered expression patterns in MYCN-amplified tumors and cell lines, and binding motifs for these factors were significantly …
-
The role of the YTHDF m6A readers in neuroblastoma
… instability including the amplification of MYCN which correlates with poor clinical outcome. These features of NB suggest a pivotal role of transcriptome dynamics in this disease. Analyses of 1089 NB patients show that an overall increase in m6A-regulatory factors correlates with the worst …
-
Genomic Characterization of Recurrent Chromosomal Aberrations in Retinoblastoma
… exhibits high-level amplification of the MYCN gene on chromosome 2p and is characterized by pRb expression and aggressive histology. 1p36 deletion is also associated with MYCN amplification in neuroblastoma. Our project is testing the hypothesis that 1q and 6p gain and 2p amplification – …
-
The novel long noncoding RNA lncUSMycN in N-Myc-induced oncogenesis
… of a 130 kb genomic DNA region containing the MYCN oncogene. While MYCN has been extensively investigated, it is unknown whether other genomic elements within the 130 kb amplicon play a role in neuroblastoma. In this study, I experimentally validated the novel long noncoding RNA up-stream of …
-
Identification Of Neuroblastoma And Its Prognostic Markers Using Raman Spectroscopy
… on the amplification of certain genes, including MYCN. Raman spectroscopy is a physics-based method which identifies the biochemical fingerprint of a sample. It has recently been applied to disease classification, specifically in adult cancers.</p> <p><strong>Methods</strong>: To identify …
-
Inhibition of KDM4 with QC6352 to Target High-Risk Neuroblastoma
… 15% of all childhood malignancy-related deaths. MYCN amplification, occurring in 20-25% of all NB patients and 50% of all high-risk NB patients, is the well-characterized genetic abnormality and is associated with ill-fated outcomes. Regrettably, in spite of the contemporary therapeutic advances …
-
The critical role of histone methylation in tumour progression and as anti-cancer targets in neuroblastoma
… in tumours correlated with high levels of MYCN gene expression and poor patient survival independent of MYCN amplification, age at diagnosis and disease stage. I have also demonstrated that JMJD6 up-regulated both N-Myc and c-Myc in neuroblastoma cell lines. Conversely N-Myc and c-Myc did …
-
DEGRADATION OF XIAP AS A NOVEL TREATMENT STRATEGY IN HIGH-RISK NEUROBLASTOMA
… and prolonged the overall survival of high-risk MYCN-amplified neuroblastoma patient-derived xenografts. Collectively, this presents the degradation of XIAP mediated by ARTS mimetics as a potential therapeutic strategy for treatment of neuroblastoma, specifically aggressive high-risk …
-
Comprehensive Integrated Genomic and Histopathological Analysis of Paediatric Diffuse Intrinsic Pontine Glioma
… distinct subgroups (H3-K27M, Silent and MYCN). The H3-K27M subgroup is highly p.Lys27Met histone H3 mutated and associated with additional hits including activating mutations in ACVR1, frequent RB1 and TP53 deletions, PVT-1/MYC or PDGFRA gains/amplifications, genomic instability and …
-
Characterizing ALCAM as an oncoprotein and immunotherapeutic target in neuroblastoma
… (ChIP)-sequencing data for the oncoprotein MYCN showed binding of MYCN to the ALCAM promoter. We used Promoter Capture-C data and discovered a region approximately 10 kb upstream of the ALCAM promoter that was predicted to be an enhancer. Using luciferase reporter vectors and the MYC(N)-MAX …
-
Entwicklung neuer molekularzytogenetischer Verfahren für die Tumordiagnostik
… (Zelllinien: 1p36, 1q12 (Kontrolle), 2p24 (MYCN), 11p15 (Kontrolle), 11q23, 17p12 (Kontrolle) und 17q25; Gewebeschnitte: 1p36, 2p24 (MYCN), 11q23 und 17q25). Außerdem wurden spezielle Protokolle entwickelt, um eine möglichst hohe Hybridisierungseffizienz zu erreichen. Zellkerne beider …
-
Reductions in expression of growth regulating genes are observed in skeletal muscle and organs with age
… growth regulating genes (Ezh2, Gpc3, Mdk, Mest, Mycn, Peg3, and Plagl1) in male and female skeletal muscle, heart, and liver with age. It was hypothesized that the expression of these genes would differ not only between sexes, but also between tissues. Female and male C57BL/6J mice were …
-
Biochemical Context Drives Inhibitor Selectivity in Aurora A Kinase
… scaffold for N-Myc, the protein product of the MYCN gene, which is heavily amplified in many childhood neuroblastomas and late-stage neuroendocrine prostate cancers. A strategy to block growth of cells harboring MYCN amplification is to disrupt complex formation between N-Myc and Aurora A, as …
-
Identification of Familial Wilms Tumor Predisposition Genes Using Whole Genome Sequencing
… germline duplication including <em>NBAS, DDX1, MYCN </em>and <em>MYCNOS</em> was identified in 4% of families, as well as germline missense mutations in <em>DICER1 </em>(4% of families) and <em>SPHK2</em>. Functional studies demonstrate that the missense mutation identified in <em>SPHK2 …
-
Targeting the N-myc oncoprotein using nanobody technology
… with the most aggressive forms. For example, MYCN, the gene encoding N-myc, is amplified in 20-30% of neuroblastomas, and amplification strongly correlates with advanced stage and poor prognosis. Myc proteins are therefore considered “most wanted” targets for cancer therapy, but have long been …
-
TCF4 Is a Key Mediator of Cell Identity and Oncogenesis in Neuroblastoma
… direct transcriptional regulation of a MYC/MYCN oncogenic program. To identify potential therapeutic vulnerabilities, we characterized the TCF4 regulatory interactome and identified multiple epigenetic factors including HDACs and KDM1A. We determined that inhibitors to both HDACs and KDM1A, …
Page 1 of 2