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Showing 1 to 20 of 144 for “"MTT assay"”.
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Physical and Biologic Properties of Calcium Silicate Endodontic Sealers
… SEALER. MATERIALS AND METHODS: Nuclei counting assay and MTT assay were used to determine the cytotoxicity of the sealers at dilutions of 1:1, 1:4, and 1:32 and at 24 hours, 72 hours, and 1 week. Vickers microhardness was determined using a micro indenter. Solubility was tested after immersion …
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VIABILITY OF CERIUM OXIDE NANOPARTICLES AS A RADIOPROTECTOR AGAINST PROTON RADIATION FOR NON-MALIGNANT CELLS
… was measured with the Microculture Tetrazolium (MTT) assay 24 and 48 hours post irradiation. DNA damage was determined using the Terminal Uridine Nick-End Labeling (TUNEL) assay. While showing promise for potential radioprotection of non-malignant cells using both assays, the MTT assay results …
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Cytotoxic compounds from the genus Centaurea.
… respectively, by the brine shrimp lethality assay, MTT assay on human colon cancer cell line (CaCo-2) and DPPH assay. Among all the species, the methanol extract of C. bornmuelleri, C. gigantea, C. huber-morathii and C. montana were the most toxic extracts in brine shrimp lethality and MTT …
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Effects of Green Tea Extract (GTE) for Normal and Cancer Cell Death by Radiation
… 다양한 농도의 녹차추출물을 단독으로 처리하거나 녹차추출물 처리 후 방사선을 조사하고 MTT assay, clonogenic assay, ROS assay를 시행하였다. 방사선과 함께 0.025 및 0.05 mM의 녹차추출물을 사용하였을 때 방사선만을 조사받은 경우에 비해 정상 세포주인 IEC-6에서 방사선조사 후 세포생존율이 증가하였으며 방사선조사를 받지 않은 경우에도 같은 비율로 세포생존율의 증가가 있었다. 대장암 세포주인 HCT-116에서는 0.025 및 0.05 mM 녹차추출물 처리로 방사선에 의한 세포살상이 …
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불멸화 유전자 도입을 통한 베타 세포주 구축
… lenti hTERT virus의 transduction 16 5. Soft agar assay 16 6. Immunocytochemistry 16 7. BrdU incorporation assay 16 8. 단일 세포 선별 18 9. 인슐린 정량 18 10. RT-PCR 18 11. MTT assay 19 Ⅲ. 결과 23 Ⅳ. 고찰 49 Ⅴ. 결론 54 참고문헌 55 ABSTRACT 59"
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Investigations on the Effects of Palmitate on Neuronal Cells
… palmitate (PA) and examined cell viability using MTT assay. Concentrations of PA at or above 200 μM in the growth media were associated with a decrease in cell viability. In order to examine whether N-acetylcysteine (NAC) or sodium phenylbutyrate (PB) affected the PA-induced decrease in cell …
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Investigating the anti-proliferativeproperties and their mechanism(s) of actionof marine derived glycosaminoglycans fromtwo marine sources
… was also performed on the prawn GAGs. The MTT assay was employed to also investigate the chemotherapeutic differences between the two derived GAGs on K562 cancer cells. Furthermore, the MTT assay was employed to investigate the chemotherapeutic properties of the prawn derived GAGs on …
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Anti-proliferative effect of methyl gallate isolated from mangifera pajang kosterman in selected cancer cell lines
… bromide (MTT) assay against breast (MCF-7 and MDA-MB-231), prostate (PC-3), pancreatic (Capan-2) and colon (HCT-116) cancer cell lines. The effect of methyl gallate on cell cycle arrest and apoptosis were confirmed by using cell cycle analysis and Annexin V …
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The role of Thyroid hormone Transporters Monocarboxylate Transporter 10 (MCT10) and Monocarboxylate Transporter 8 (MCT8) in extravillous trophoblasts.
… 1) Cell proliferation was assessed by the MTT assay at 24-96hrs. Gene expression was assessed by quantitative QPCR (2-48h). 2) MCT8 was down-regulated using siRNA, and MCT8 and MCT10 were over-expressed by plasmid transfection. Effects of altering expression were assessed by MTT assay …
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Effect of broccoli bioactives on prostate cancer cell invasion in vitro
… and counted. The results of Matrigel invasion assay indicate that the invasive ability of DU145 cells was significantly inhibited by the physiologically relevant levels of SF (3-5 µM) and I3C (120-200 nM), but not NGB (1-50 µM) or Q (1-5 µM). We further performed the MTT assay to examine …
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Assessment of synthetic chalcones as cancer chemopreventive agents
… using thiazolyl blue tetrazolium bromide (MTT) assay, and (ii) chemopreventive activity by luciferase fold induction using luciferase assay through the AREc32 reporter cell line, which includes a luciferase gene linked with ARE drive gene. The results showed that twelve chalcones have …
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Investigations on the effects of Typha capensis on male reproductive functions
… morphology and viability (determined by means of MTT assay). Part 2: The crude extract HPLC profiles were obtained by preparing the extracts for different seasons (Autumn, Winter, Spring, Summer). TM3-Leydig cells, LNCaP cells and PWR-1E cells incubated with different concentrations T. capensis …
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Osteogenic and chondrogenic differentiation of rat bone marrow stromal cells in 3D culture with novel PCL-TCP scaffolds
… phase-contrast microscope, confocal microscope, MTT assay, and SEM. Osteogenic differentiation was substantiated by Von Kossa staining, heightened expression of ALP and osteocalcin; chondrogenic differentiation was confirmed by alcian blue staining and toluidine blue staining. In conclusion, it …
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Cell Toxicology Study of RRR-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (TPGS).
… 5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate cell viability. Cell death can occur through necrosis or apoptosis. Our results suggested that TPGS triggered apoptotic cell death. Induction of apoptosis, as measured by caspase 3 enzymatic activity, was dependent …
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Staphylococcus aureus의 Lipoteichoic acid(LTA)에 의한 사람의 정상 코 점막 상피세포에서의 MMP와 TIMP의 발현
… B. Lipoteichoic acid(LTA) 처리 C. MTT assay D. RNA 추출 및 cDNA 합성, PCR E. Enzyme-linked immunosorbent assay(ELISA) F. Gelatin zymography 1. Gelatinase B의 준비, 활성화 2. Gelatinase B의 전기영동, 기질-효소 간 반응 3. 발현된 band의 염색, 결과의 수치화 G. Western blot 1. LTA 처리, 단백질 정량 2. 단백질의 전기영동, Antibody 반응 H. …
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Investigation of the efficacy of identified acetolactate synthase inhibitors, peptidyl cysteine protease inhibitors, thiolactomycins, and thiosemicarbazone compounds against Mycobacterium tuberculosis
… using the resazurin microtitre plate assay afterwhich active compounds were assessed for cytotoxicity in vitro against elicited peritoneal macrophages using the MTT assay. Of the 38 acetolactate synthase inhibitors tested, 2 derivatives namely RKG162A and RKG1541 were bactericidal …
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Physical and biological properties of a Pt(ll) complex of a beta-diketiminate ligand with pendant quinoline arms
… titrations and an ethidium-bromide displacement assay. Our results indicate that the compounds interact with DNA, although the mode of interaction is still ambiguous. Fluorescence studies were also performed to investigate the interaction of the complex with bovine serum albumin (BSA) as a model …
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The Effect of Drugs of Abuse and HIV-1 GP120 on Autophagy in Astrocytes
… of autophagy in chronic cocaine exposure using MTT assay and western blot analysis. In the second chapter, the effect of methamphetamine on autophagy in SVGA astrocytes, as well as human fetal astrocytes were examined. The mechanistic pathway of methamphetamine-mediated autophagy was determined. …
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Biocompatibility and Bioactivity of Calcium Silicate-based Dental Bioceramics
… observations of cell<br/>2) Cell viability assays<br/>3)Immunocytochemistry<br/>4)Protein Biochemistry<br/>5)mRNA expression analysis<br/><br/>Visual observations demonstrated differing changes in cellular morphology between our two main test materials and cell lines, with gingival …
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Proliferationsverhalten kultivierter Mesangialzellen und glatter Gefäßmuskelzellen nach Stimulation mit Angiotensin II und atherogenen Lipoproteinen : Rezeptorbeteiligung
… markiertem 3H-Thymidin-Einbau als auch durch den MTT-Assay, der auf der photometrisch messbaren Umwandlung von 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazoliumbromid beruht, quantifiziert. Die Rezepto-ren wurden auf Proteinebene durch Western Blot Analysen nachgewiesen. Zum Nachweis der …
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