Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 13 of 13 for “"MS-based proteomics"”.
-
Outlining different perspectives for accurate MS-based proteomics in processed matrices - a case study on European feed control
… partially ensure compliance. Mass spectrometry (MS) offers a promising approach to ensure food and feed safety, though thermal processing may compromise its sensitivity. Hence, this thesis explores strategies to enhance MS-based analysis in thermally processed matrices, addressing key challenges …
-
Proteomics of physiologically relevant stresses on model mycobacterium tuberculosis organisms, and the effects on virulence
… high resolution tandem mass spectrometry (LC-MS/MS) based proteomics, to study some of the environmental challenges that Mycobacterium tuberculosis may encounter within the host, in the model organisms Mycobacterium smegmatis and Mycobacterium bovis BCG. These include sub-lethal concentrations …
-
Benchmarking methods for label-free quantitative proteomics data analysis
… disease-mediating proteins. Mass spectrometry (MS)-based proteomics is the predominant technology for protein quantification. In particular, MS-based label-free quantification (LFQ) proteomics can quantify thousands of proteins in complex biological samples. However, the molecular heterogeneity …
-
Biochemical characterizations of extracellular vesicles shed by vegetative and sporulating Bacillus subtilis
… cells and characterized using mass spectrometry (MS)-based proteomics, microscopy, and fluorescence spectrophotometry. The microscopic approach demonstrated that both vegetative and sporulating cells produce EVs. In the proteomic analysis, 156 proteins were identified with statistical significance …
-
MS-Based Chemical Proteomics Studies of Extracellular Glycoproteins: Identification, Quantification, and Dynamics
… glycoproteins, through mass spectrometry (MS)-based proteomics. First, an enrichment method based on metabolic labeling with a sugar analog, copper-free click chemistry, and MS-based proteomics is employed to target cell-surface glycoproteins from multiple cell lines. The method is …
-
A flexible and versatile framework for statistical design and analysis of quantitative mass spectrometry-based proteomic experiments
<p>Quantitative mass spectrometry (MS)-based proteomics is an indispensable technology for biological and clinical research. As the proteomics field grows, MS-based proteomic workflows are becoming more complex and diverse. The accuracy and the throughput of the MS measurements and of the signal …
-
A Global and Targeted Proteomic Investigation of Aspergillus fumigatus
… (n = 42) of A. fumigatus. Utilisation of MS-based proteomics facilitated the identification of proteins typically under-represented in 2D-PAGE proteome maps, including proteins with multiple transmembrane regions, hydrophobic proteins and proteins with extremes of molecular mass and pI. …
-
Development of ion mobility mass spectrometry deconvolution strategies for use in data-independent acquisition proteomics.
… in ion mobility (IM) and mass spectrometry (MS) resolving powers can limit the success of MS-based proteomics approaches. This work highlights the development of IM-MS deconvolution techniques and their utilization with commercial data processing software to improve results from IM-enhanced …
-
Proteomic characterisation of Estrogen Receptor (ERα) interactome in breast cancer
… the last decade, quantitative mass spectrometry (MS)-based proteomics has become a powerful tool for the study of protein interactions. However, technical challenges have restricted most applications to qualitative observations and the dynamics of protein interactomes have until recently been …
-
An in vivo study of chromatin compaction in human cells
… I have performed experiments to study charge-based compaction of chromatin in intact nuclei. Studies on permeabilised cells indicate that compaction of chromatin increases with an increase in concentration of polyvalent cations. This increase in chromatin compaction is charge and concentration …
-
Characterization of human foreskin Langerhans cells
… chromatography with tandem mass spectrometry (LC-MS/MS). Results: Langerhans cells were an average of 85% pure post-sorting. The numbers of Langerhans cells between the inner FS vs. outer FS were not statistically different (mean: 0.56% vs. 0.68% (SD=0.37) from migratory cells and 0.28% vs. 0.45% …
-
Genome-scale Precision Proteomics Identifies Cancer Signaling Networks and Therapeutic Vulnerabilities
<p>Mass spectrometry (MS) based-proteomics technology has been emerging as an indispensable tool for biomedical research. But the highly diverse physical and chemical properties of the protein building blocks and the dramatic human proteome complexity largely limited proteomic profiling depth. …
-
Proteomics studies of protein homeostasis and aggregation in ageing and neurodegeneration
… possible by major advances in mass spectrometry-based (MS) proteomics, which allows the identifications and quantification of thousands of proteins in a variety of biological samples. In the first part of this thesis, I analyse proteome-wide MS data for the nematode worm C. elegans upon ageing, …