Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 65 for “"MRNA stability"”.
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Enhancing mRNA stability through the addition of stabilizing untranslated regions
Messenger-RNA (mRNA) therapy, in which mRNA is introduced to cells or tissues to cause a transient expression of specific genes, has applications ranging from tissue engineering to neural regeneration. This transient nature of mRNA, however, limits the duration of gene upregulation, and potentially …
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mRNA stability in soma and neurites of cultured neuronal cells
Neurons rely on mRNA localization and local protein synthesis in neurites to regulate synaptic plasticity, axonogenesis and neural signaling. The local regulation of mRNA stability in neurites is poorly understood. To determine mRNA decay rates, we analyzed the subcellular transcriptomes of neural …
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A Nascent Peptide Code for Translational Control of mRNA Stability in Human Cells
Stability of eukaryotic mRNAs is associated with their codon, amino acid, and GC content. Yet, coding sequence motifs that predictably alter mRNA stability in human cells remain poorly defined. Here, we develop a massively parallel assay to measure mRNA effects of thousands of synthetic and …
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Molecular Mechanism of the Stimulation of Alkaline Phosphatase Activity in Human Bone Cells by 1,25(OH)2 D3
… increase in steady state level of ALP mRNA, showing a temporal cause-and-effect relationship between the two parameters.</p> <p>Evaluations of the mechanistic action of 1,25(OH)<sub>2</sub>D<sub>3 </sub>showed that the increase in the steady state level of ALP mRNA was a result of both …
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Transcriptional and Post-transcriptional Control of Nhlh2 with Differing Energy Status
… the SNPs located at position 1568 in the NHLH2 mRNA (NHLH2A<sup>1568G</sup>) which converts adenosine to guanine might have the potential to decrease the mRNA stability. For more investigation about this SNP, the mouse Nhlh2 tail was cloned into 2 different vectors and these vectors were …
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Analysis of cis-acting expression determinants of the tobacco psbA 5’UTR in vivo
… regulated at the posttranscriptional levels of mRNA stability and translation efficiency. The expression of psbA, an important photosynthesis-related chloroplast gene, has been revealed to be regulated via its 5’- untranslated region (UTR). Some cis-acting elements within this 5’UTR and the …
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Analysis of the hex regulon in Pseudomonas aeruginosa
… without HexR control. Analysis of zwf mRNA showed that Crc affects mRNA stability and is acting at a post-transcriptional level.
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Messenger RNA Destabilization by -1 Programmed Ribosomal Frameshifting
… genomic sequences, and suggested a role in mRNA stability. This work improves and extends the computational methods used to search for potential -1 PRF signals. It continues to examine four yeast -1 PRF signals and show that they promote significant mRNA destabilization through the nonsense …
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Sindbis virus usurps the cellular HUR protein to stabilize its transcripts and promote infections of mammalian and mosquito cells
… RNAs of Sindbis virus strongly resemble cellular mRNAs as they are capped at their 5’ ends and polyadenylated at their 3’ termini. These features allow the viral RNAs to act like cellular mRNAs and make them prime substrates for the cellular mRNA decay machinery. Sindbis virus RNAs are indeed …
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Identification and characterization of a small molecule inhibitor of IMP-1 that decreases expression of IMP-1 target mRNAs and inhibits proliferation of IMP-1 positive cancer cells
… proteins is involved in translation control, mRNA localization and mRNA stability. I have studied the Insulin-like Growth Factor-2 mRNA-Binding Protein 1 (IGF2BP1/IMP-1/CRD-BP). IMP-1 exhibits an oncofetal pattern of expression, where it is expressed in embryonic development and its expression …
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Quantitative analysis of translational coupling in Escherichia coli
Multiple genes are often transcribed on the same mRNA transcript in bacteria. These multi-gene transcriptional units, called operons, are widespread in bacteria with nearly half of all annotated genes predicted to reside in them. How operons affect translational regulation is still not known. There …
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Apobec-1 Complementation Factor (ACF) Binds and Regulates Multiple RNAs
… range of transcripts that function to regulate mRNA stability, localization, and/or translation through interaction with ARE-Binding Proteins. Apobec-1 Complementation Factor: ACF) was originally identified as a co-factor in ApoB mRNA C to U editing, but has recently been implicated in …
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Nonlinear Feedback and Stochastic Control in Translation
… called translation, in which a ribosome decodes mRNA, a single-stranded copy of DNA, to produce a specific amino acid chain. <br/>Given the essential role of proteins in maintaining life, it is of central importance to comprehend the translation process and how it is regulated. Translation …
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Genomic and non genomic effects of elevated concentration of anabolic steroids in human neuronal cells
… revealed that nandrolone may regulate MOPr mRNA stability. In SH-SY5Y cells transfected with a human MOPr luciferase promoter/reporter construct, nandrolone did not alter the rate of gene transcription. These results suggest that nandrolone may regulate MOPr expression through …
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Targeting CDK to Prevent and Treat Retinoblastoma and other Cancers
… Protein Processing networks (e.g. translation, mRNA stability, splicing, and protein targeting). Through mining publically available datasets, we unravel a novel co-regulatory/co-dependent relationship between CDK2 activity and Protein Processing networks. Leveraging Protein Processing networks, …
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Alternative Polyadenylation Modulates Expression of Pro-Fibrotic Proteins and Contributes to Lung Fibrosis
… 3’UTR shortening via APA leading to enhanced mRNA stability and protein translation. This NUDT21 reduction promotes tumor growth in glioblastoma by enhancing expression of oncogenes. Cancer and IPF share cellular features, such as enhanced expression for pathological mediators and increased …
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Regulation of Synergistic Inducible ABIN-3 Gene Expression in Response to IL-10 and LPS
… level involving an increase in its mRNA stability. These data indicate that IL-10 synergizes with LPS to induce ABIN-3 gene transcription as opposed to inducing stabilization of its mRNA. Future studies will aim to determine the minimal cis-acting elements within the ABIN-3 gene …
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Regulation of human sex-linked homologs DDX3X and DDX3Y and phenotypic consequences
… levels. This regulation is mediated through mRNA stability and buffers total levels of DDX3X and DDX3Y protein in human cells. Our findings indicate that gene regulatory mechanisms present on ancestral autosomes were retained and modified during the 200-million-year evolution of the human sex …
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A SYNTHETIC TMRNA PLATFORM FOR ELUCIDATION OF BACTERIAL PROTEOME REMODELING UNDER STRESS
… bacterial stress response and is a function of mRNA stability, protein turnover and proteolysis. Total proteome measurements give a view of the stable proteome but can fail to capture dynamic changes under stress, including incomplete polypeptides that result from cleaved mRNAs or stalled …
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Construction and analysis of novel controllable expression vectors for Bacillus subtilis
… established, which allowed to study promoter and mRNA stabilizing elements to enhance the transcript level and mRNA stability leading to higher production of recombinant protein. During the development of the glycine-inducible and lysine-autoinducible expression plasmids, the presence of a small …
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