Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"MRNA localization"”.
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mRNA localization in Caenorhabditis elegans embryogenesis
… output, post-transcriptional regulation of mRNA is essential for life. As a result, many mechanisms underlying post-transcriptional regulation are highly conserved across the kingdoms of life. As the spatial resolution of microscopy and sequencing assays has increased, mRNA localization has …
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Neuronal Polarization and Axonal mRNA Localization on Microfabricated Substrates
… a PDMS microdevice to isolate purified axonal mRNA transcripts for microarray analysis. The list of axonal mRNAs generated will facilitate further studies of protein synthesis within axons, which is now a recognized process in normal axon physiology.
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Analysis of the role of Drosophila IMP in actin mRNA localization and cell migration
… for IMP function is that it localizes actin mRNA to the leading edge of migrating cells. mRNA in situ for the two cytoplasmic actins in Drosophila (actin42A and actin5C) shows that both are localized to the front of the migrating border cell cluster as is IMP protein. Border cells mutant for …
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Biochemical purification and functional characterization of the She RNP complex from S. cerevisiae
Asymmetric mRNA localization is a widely used mechanism to sort cell fate determinants in development. In the budding yeast Saccharomyces cerevisae ASH1 (for 'asymmetric synthesis of HO') mRNA localization to the tip of the growing bud leads to targeting of Ash1p to the daughter cell nucleus prior …
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Functional characterization of the role of Imp, a Drosophila mRNA binding protein, during oogenesis
… regulatory mechanisms, including mRNA localization and translational control. A family of highly conserved RNA binding proteins in vertebrates, VICKZ (V̲g1RBP/V̲era, I̲MP-1, 2, 3, C̲RD-BP, K̲OC, Z̲BP-1) proteins, has been shown to act in these two processes. Previous studies of the …
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Analyses of a genic male-sterile mutant in soybean
… pattern, phylogenetic studies, and in situ mRNA localization studies (in situ mRNA localization is in a separate manuscript not included in the dissertation), Sglu7 is the candidate gene that encodes [beta]-1,3-glucanase expressed in the anther tapetum and involved in male sterility.</p>
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Single Cell Transcriptomics and Spatial Localization in Mouse Neurons
… molecular organization, partly mediated by mRNA localization. Consequently, tremendous efforts have been made to identify which mRNA species are present and enriched in the soma and dendrites. However, these studies do not describe the spatial distribution and fine scale localization of the …
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mRNA stability in soma and neurites of cultured neuronal cells
Neurons rely on mRNA localization and local protein synthesis in neurites to regulate synaptic plasticity, axonogenesis and neural signaling. The local regulation of mRNA stability in neurites is poorly understood. To determine mRNA decay rates, we analyzed the subcellular transcriptomes of neural …
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Deciphering tranSNPs: functional genetic variants shaping protein output and cancer-related phenotypes
… imbalanced across polysome-bound and total mRNAs. We provide a comprehensive characterization of tranSNPs and their implications by exploring their cell line and cancer-type specificity, as well as their mechanistic insights focusing on the rs1053639 tranSNP (T/A) on DDIT4 3’UTR. This …
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RNA Localization and Translational Regulation on the Endoplasmic Reticulum
<p>mRNA localization is emerging as a critical cellular mechanism for the spatiotemporal regulation of protein expression and serves important roles in oogenesis, embryogenesis, cell fate specification, and synapse formation. Signal sequence-encoding mRNAs are localized to the endoplasmic reticulum …
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Functional Roles of Nova in RNA Metabolism
… an intron of the glycine receptor a2 subunit pre-mRNA. Evidence that Nova-1 acts as a regulator of alternative splicing in transfected cell lines is presented. Furthermore, a direct role of Nova-1 on splicing is demonstrated by establishing an in vitro assay for Nova-1's regulatory role in …
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Regulation of mRNA decay by Pumilio in Drosophila melanogaster
… development is driven by maternally loaded mRNAs synthesized in the female parent during oogenesis. These maternally synthesized mRNAs are regulated by post-transcriptional mechanisms, including mRNA localization, degradation and translational activation and repression. Many of these …
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Structure-function studies of human SLC36 amino acid transporters
… with no information available except their mRNA localization in various tissues. Additionally there is no structural information available about the PAT family of transport proteins. The thesis has two broad aims. The first aim was to study the orphan PAT transporters and to investigate …
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A role for RNA localization in the human neuromuscular disease myotonic dystrophy
RNA localization, a regulated step of gene expression, is fundamentally important in development and differentiation. In multidisciplinary experiments, we discovered that RNA (mis)localization underlies the human disease myotonic dystrophy (DM). DM, the most prevalent adult muscular dystrophy, is …
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… the 5'-untranslated region (5'-UTR) of a given mRNA, translation of a downstream coding sequence is directly controlled by TetR and tetracycline analogs. In endogenous and synthetic 5'-UTR contexts, this modular system efficiently regulates the expression of multiple target genes, and is …
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Towards understanding the mechanisms behind mRNA localisation in growing axons
During embryonic nervous system assembly, mRNA localisation is precisely regulated in growing axons, affording subcellular autonomy by allowing controlled protein expression in space and time. Different sets of mRNAs exhibit different localisation patterns across the axon. Little is known, however, …
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Programming Molecular Devices using Nucleic Acid Hairpins
… for RNA folding, protein recognition signals, mRNA localization and regulation of mRNA degradation. On the other hand, </p><p>DNA hairpins in biological contexts have been studied with respect to forming cruciform structures that can regulate gene expression.</p><p>The use of DNA hairpins as …
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Identification and characterization of a small molecule inhibitor of IMP-1 that decreases expression of IMP-1 target mRNAs and inhibits proliferation of IMP-1 positive cancer cells
… proteins is involved in translation control, mRNA localization and mRNA stability. I have studied the Insulin-like Growth Factor-2 mRNA-Binding Protein 1 (IGF2BP1/IMP-1/CRD-BP). IMP-1 exhibits an oncofetal pattern of expression, where it is expressed in embryonic development and its expression …
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Understanding Huntington's Disease pathogenesis using next generation sequencing analyses
… developed to examine translational control and mRNA localization in the brain of Huntington's disease mice. Concurrent Ribo-Seq and RNA-Seq in diseased and wildtype animals would answer if there was altered translational control. The Ribo-Seq protocol designed in cell culture was optimized for …
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Commonalities between SMA and ALS: investigation of ribosome heterogeneity and translational defects
… capable of finely tuning translation of selected mRNAs, do exist. Multiple types of ribosomes are defined by several elements, such as their composition, and their association with different proteins, known as ribosome-associated proteins (RAPs). In particular, these proteins can exert a direct …