Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"MLV"”.
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Structural determinants of murine leukemia virus (MLV) reverse transcriptase (RT) important for fidelity and drug resistance in vivo
… structural elements of murine leukemia virus (MLV) RT important for fidelity and drug-resistance in vivo, we developed a D17-based encapsidating cell line (ANGIE P) which is designed to express the amphotropic MLV envelope. ANGIE P cell line also contains an MLV-based retroviral vector (GA-1), …
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The inhibition of M-MLV and HIV-1 reverse transcriptase by polyphenols extracted from the resurrection plant Myrothamnus flabellifolia (Welw.)
… from Myrothamnus flabellifolia (Welw.) on M-MLV and HIV-I reverse transcriptases. The first part of the thesis was an attempt to isolate 3,4,5 tri-O-galloylquinic acid, the major polyphenol found in Namibian Myrothamnus flabellifolia plants. This polyphenol was successfully purified by column …
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Engineering Viruses for Gene Therapy: Isolating and Characterizing Murine Leukemia Virus With Improved Stability
… genome of amphotropic murine leukemia virus (MLV) and selected for improved stability and infection at 37°C. After one round of mutagenesis and several rounds of selection, we isolated MLV variants with double the half-life of wild-type MLV. The improved stability of the mutant MLV leads …
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Structural determinants of murine leukemia virus reverse transcriptase that are important for template switching, fidelity, and drug resistance
… mutational analysis of manne leukemia virus (MLV) RT to identify structural elements important for template switching. Based on obtained results, we proposed a dynamic copy-choice model in which both the rate of DNA polymerization and the rate of degradation of the RNA template influence the …
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Mechanisms of retroviral replication
… two simple retroviruses, murine leukemia virus (MLV) and spleen necrosis virus (SNV). SNV proteins can package RNA of both SNV and MLV with similar efficiency. In contrast, MLV proteins cannot package SNV RNA. NC domain was shown to be the determinant of nonreciprocal RNA recognition by these two …
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In vitro and In vivo Characterization of the Infection Efficiency of a Modified Retrovirus Envelope Glycoprotein for Targeting Gene Transduction
… work, the Moloney murine leukemia virus (MLV) envelope glycoprotein (Env) was modified to generate the Sst-RBS glycoprotein. This glycoprotein was created by replacing the wild type (WT) receptor binding site (RBS), located on the surface subunit (SU) of the Env, with the somatostatin …
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Analysis of Porcine Immunity in PRRS Virus
… the cDNA encoding the protein with a PRRS MLV vaccine resulted in transiently enhanced IFN-gamma T cell responses in vivo. In addition, the ability of a synthetic dsRNA polynucleotide, polyinosinic acid:polycytidylic acid (poly I:C), complexed with poly-L-lysine and carboxymethylcellulose …
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Pre-eruptive conditions beneath Medicine Lake Volcano, California, during the Pleistocene epoch
… conditions beneath Medicine Lake Volcano (MLV) during the Pleistocene epoch. Prior to this investigation, the Pleistocene lavas of MLV had not been extensively studied because detailed time relations among them were not known. Recent work by Donnelly-Nolan has provided a time framework for …
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Field comparison of intranasal and injectable bovine respiratory disease vaccination on beef calf antibody concentrations, average daily gain, and bovine respiratory disease morbidity
… into an injectable modified live viral (IJ-MLV) group, intranasal homologous boost (IN-MLV) group, or intranasal heterologous boost (IN-KV) group. Vaccines were administered at birth, ‘‘turnout’’ (~2 months of age), and weaning. Blood samples and weights were collected at ‘turnout’, two …
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Biochemical Investigation of the Intracellular Trafficking of Non-Viral and Hybrid Gene Therapy Vectors
… thereby estimating endosomal escape of VLP. MLV were observed to achieve reverse transcription rapidly, with more than half of infecting viruses being reverse transcribed within 8 hours. PEI:VLP were delayed approximately 4 hours (relative to viruses) in having their RNA reverse transcribed. …
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Molecular characterization of the major envelope protein of porcine reproductive and respiratory syndrome virus (PRRSV) and evaluation of its use for a diagnostic assay, vaccine development, and the examination of quasispecies evolution
… the mid 1980s. Although modified live vaccines (MLVs) are typically used for the prevention of clinical disease, they are not always fully effective. Additionally, acute PRRS outbreaks, characterized by more severe clinical signs, have appeared in herds that were previously vaccinated. In this …
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Novel approaches to enhance the protective immune responses of vaccines against Porcine Reproductive and Respiratory Syndrome Virus
… an effective modified live-attenuated vaccine (MLV) with broad protection is the delayed and insufficient immune responses mounted by PRRSV, and the problem is further exacerbated by the antigenic variations of the constantly-evolving field strains of PRRSV. In order to boost the immune response …
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Design of hybrid retroviral/synthetic gene delivery vectors
… (φ) envelopes for Moloney Murine Leukemia Virus (MLV) -like particles (M-VLPs). M-VLPs are essentially intact viruses lacking the envelope protein most necessary for transfection, thus making them inactive. Both chitosan and liposomes composed of DOTAP, DOPE and cholesterol electrostatically …
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Paleovirological Analyses of Endogenous Retroviruses and Host Innate Immune Effectors
… We found that a modern gammaretrovirus (MLV) could be pseudotyped with ancHTenv enabling it to infect a wide variety of primate cell lines with titers that are similar to MLV particles carrying the amphotropic MLV envelope. A single HERV-T proviral insertion in the genome of all great …
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Analysis of factors that affect pseudotyping in retroviruses
… assembly defect of murine leukemia virus (MLV) particles in HeLa cells. The budding defect of MLV from HeLa cells is restored by fusion with 293FT cells, suggesting that an unknown necessary host factor is deficient in HeLa cells.
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Phase Behavior and Structural Transitions in The Mixtures of Cationic Surfactants and Hydrophobic Counterions
… gel (consisting of multilamellar vesicles MLV with yield value). The complex viscosity (0.01 Hz) of 100 mM CTAOH rises by six orders of magnitude as the rodlike micelles form.It decreases then to the turbid region, and then rises again approximately six orders of magnitude. The second …
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Rôle modulateur de l'oxyde nitrique dans la vasoconstriction et l'élévation du calcium intracellulaire induites par l'endothéline-1 et le thromboxane A[indice inférieur 2] dans des vaisseaux humains et de lapins
… les réponses des muscles lisses vasculaires (MLV) en utilisant le 8-Br-GMPc et des inhibiteurs de la protéine kinase G (PKG). Nos résultats montrent que le 8-Br-cGMP atténue la tension induite par l'ET-1 en préventif et en curatif sans affecter le [Ca][indice inférieur i]. Par contre, les …
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The effect of brain-specific VEGF-C deletion on vascular morphology in the brain and skull
… (dural) blood vessels or lymphatic vessels (mLV) in the central nervous system. Immunofluorescent staining was used to visualize the vasculature in dura, spinal column, pituitary gland and brain. The deletion caused no obvious effects on the blood vasculature in the analyzed tissues. However, …
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Hybrid Gene Therapy Vectors Comprised of Synthetic Polymer and Noninfectious Virus -Like Particles
… fails to mediate escape as efficiently as the MLV envelope protein, likely resulting in the degradation of internalized hybrid vector complexes. Incorporation of polymers better suited for mediating escape from the endolysosomal network could likely improve the hybrid vector efficiency.
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Characterization of virus strain- and pig age-dependence of porcine epidemic diarrhea virus: Implications for vaccine development
… be a good candidate for modified live virus (MLV) vaccine. Further, much is described about virulence differences between the two isolates in neonatal conventional pigs, but little is known in older pigs. Resulting data can then provide guidance on selecting appropriate PEDV strain to induce …
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