Global ETD Search
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Showing 1 to 14 of 14 for “"MLCK"”.
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Characterization of Smooth Muscle Myosin Light Chain Kinase Using Crystallization and Transient Kinetics
… of smooth muscle myosin light chain kinase (MLCK N1-75). As of now, the exact mechanism by which MLCK interacts with actomyosin complexes in smooth muscle is not well-understood because there is no highresolution 3-dimensional structure of MLCK available. In this project, the crystallization …
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Role of Myosin Light Chain Kinase in Binge Ethanol and Burn Injury-Induced Intestinal Barrier Dysfunction
… kDa) smooth muscle myosin light chain kinase (MLCK), an enzyme important for epithelial tight junction maintenance, has been implicated in barrier alterations after burn injury or ethanol exposure alone. With this knowledge, <underline>we hypothesize that the combination of binge ethanol …
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MATHEMATICAL MODELLING OF CALCIUM DEPENDENT ACTIVE CONTRACTION IN A GASTROINTESTINAL SMOOTH MUSCLE CELL
… activation of Myosin Light Chain Kinase (MLCK) which is the primary mechanism has been described in terms of two interacting modules - the first module describing the activation of MLCK, and the second describing cross-bridge formation between actin and myosin. Second, a hypothesis of …
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Interactions of Smooth Muscle Myosin Filaments with Actin and Myosin Light Chain Kinase: Insights into the Mechanics of Smooth Muscle Contraction
… of the RLC of SMM by myosin light chain kinase (MLCK), SMM filaments and actin (thin) filaments slide over one another and the cell contracts. The effect of the filamentous conformation of SMM on the kinetics underlying the interactions with MLCK and actin is unknown. The filamentous structure of …
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Role of ROK and PKC in Permeabilized Rabbit Femoral Artery
… For a comparison we also used various PKC and MLCK inhibitors and repeated these experiments in intact tissues. We found that either [Ca2+]i alone does not directly activate ROK or the permeabilization technique itself disrupts the normal ROK signaling system. Secondary findings revealed that …
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Force potentiation in the MDX mouse
… and Western blots for myosin light chain kinase (MLCK) were performed. YM and YC had similar fiber type profiles (2% I, 58% IIX/D and 40% IIB). In accordance with literature but contrary to expected conditions for elevated PTP, OM had a slower fiber type profile (1.7% I, 69% IIX/D and 29% IIB) …
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Proteomic investigation of the molecular targets of mycophenolic acid in human cells
… in MLC2 phosphorylation was through effects on MLCK. Immunoblot analysis revealed that MPA increased MLCK expression both at mRNA and protein levels. To further confirm that MLCK was the key player in MPA mediated MLC2 phosphorylation and its associated TJ disruption, we pre-incubated cells with …
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Orientation of the Cardiac Myosin Regulatory Light Chain Determined by Polarized Fluorescence
… of RLC by myosin light<br/>chain kinase (MLCK) modulates the force and speed of muscle contraction, but the mechanisms of RLC‐mediated regulation in striated muscles are less well<br/>understood than those of Ca2+‐dependent regulation via troponin in the thin<br/>filament.<br/>To help …
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Giardia Cathepsins and Their Role in Intestinal Disease
… sustained over time, at least in part, by host MLCK. Overall, this study establishes a reliable model for studying roles of parasitic cysteine proteases during host-parasite interactions. Further understanding of these proteases may pave the way for therapeutic development.
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Spatiotemporal organization, regulation and function of traction during neutrophil chemotaxis
… leading edge requires myosin light chain kinase (MLCK)-mediated myosin II contractility and is necessary for α5β1-integrin activation and leading edge adhesion. Localized myosin II activation induced by spatially activated small GTPase Rho and its downstream kinase p160-ROCK, as previously …
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The influence of oxidised low density lipoproteins on platelet activation
… to a Ca²⁺ dependent activation of MLC kinase (MLCK) and the Rho kinase dependent inhibition of MLC phosphatase (MLCP). OxLDL induced platelet shape change correlated with MLCSer¹⁹ phosphorylation. OxLDL also stimulated a tyrosine kinase pathway involving the proteins Syk and PLCγ2, which were …
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Endothelin-1 and Hypoxic Vascular Remodeling in Ovine Fetal Cerebral Arteries
… Additionally, Myosin Light Chain Kinase (MLCK) and Smooth Muscle Alpha Actin (SMαA) colocalization data shows that ET-1 promotes contractile dedifferentiation in normoxic but not hypoxic MCAs in a PKC, CaMKII, and p38 dependent manner. These results support the notion that chronic hypoxia …
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Molecular Mechanism of Zipper Interacting Protein Kinase
… regulated myosin light chain kinase (MLCK) and smooth muscle myosin phosphatase (SMPP-1M) determine the amount of force generated by smooth muscle. The calcium-independent signaling mediated by myosin phosphatase is regulated by several kinases which include zipper interacting protein …
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The influence of acute, chronic and lifelong hypoxia on the functional and structural integrity of the neurovascular unit
… artery blood velocity<br/>MHz – Megahertz<br/>MLCK – Myosin light chain kinase<br/>mmHg – Milimettres of mercury<br/>MRI – Magnetic resonance imaging.<br/>NaOH – Sodium hydroxide<br/>NF-L – Neurofilament Light Chain<br/>NINDS – National Institute of Neurological Disorders and Stroke<br/>NIH – …