Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"MHCII"”.
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Contribution Of Non-Classical Antigen Presentation To Cd4+ T Cells During Orthopoxvirus Infection
… and an antigenic peptide in complex with an MHCII molecule on an antigen presenting cell. However, the primary means by which these peptides are generated and loaded onto MHCII molecules is debated. Historically antigen presenting cells have been thought to utilize the classical pathway of …
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Understanding antigen processing in chickens using genome editing technology
… and the dedicated chaperone DM for the MHCII molecules. In mammals, these critical antigen loading molecules have limited polymorphism, providing peptides for a multigene family of well-expressed classical MHC molecules. Outside of mammals, the chicken is the only system in which peptide …
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Klonierung und Charakterisierung des Zytokins Interleukin-18 sowie der Zelloberflächenproteine CD3 epsilon, CD4, CD8 alpha und MHCII beta der Ente : Generierung von Werkzeugen für immunologische Studien im Enten-Hepatitits-B-Virus-Modell
… CD3e, CD4 und CD8a sowie die MHCIIb-Kette der Peking- und der Warzenente kloniert. Gegen DuCD4 und DuCD8a wurden sowohl polyklonale Kaninchenseren als auch monoklonale Mausantikörper erzeugt. Mit Hilfe der monoklonalen Mausantikörper und eines monoklonalen Rattenantikörpers, …
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Expression Profiles of Molecules Involved in Antigen Presentation in Gangliosides-stimulated Primary Astrocytes from Rat brain
… (CIITA) and class II major histocompatibility (MHCII) molecules, using RT-PCR and real-time PCR. Gangliosides induce CIITA mRNA expression, followed by MHC II in astrocytes, but not in microglia. Critical for the development of mature APC capability is surface expression of class II MHC …
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Imaging innate immune cell behaviour in lymphoid organs
… via major histocompatibility complex class II (MHCII) expression for antigen presentation. Secondary lymphoid organs, including lymph nodes (LNs) and Peyer’s patches, are ideal sites for innate-adaptive cellular interactions. Whilst neutrophils migrate to LNs following inflammation, whether this …
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Antigen presentation: Influence of Cell Type and Route of Antigen Uptake
… DC-like features such as expression of CD11c, MHCII and co-stimulatory molecules, yet their role in T cell activation is still a matter of investigation. Cells similar to DCs can also be produced in vitro by culturing monocytes with granulocyte-macrophage colony-stimulating factor (GM-CSF) or …
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Characterization of Dendritic Cells in the Bovine Mammary Gland
… and antigen presentation markers, including MHCII and CD80. A primary cell culture model was used to evaluate changes in transcription of CD80 and CCR7 after LPS stimulation. Cell cultures contained digested and Ficoll separated mammary tissue or supramammary lymph node tissue. While the …
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Selective transfer of exosomes from oligodendrocytes to microglia by macropinocytosis
… von Haupthistokompatibilitätskomplex II (MHCII) Komplexen, wohingegen der verbliebene Anteil an Mikroglia keine MHCII Moleküle exprimiert. Interessanterweise erfolgt die Aufnahme von Exoso! men vor allem durch durch Mikroglia ohne Antigen-Präsentationsfähigkeiten. Von diesen Ergebnissen …
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Investigating pulmonary vascular B cells
… via CD18-mediated interactions, labelling them MHCII+AnnexinV+, and that in the absence of B cells, the lung will develop pathological fibrotic interstitial lung disease. In this body of work, we determined that pulmonary intravascular B cells can marginate which allows them to specialize in …
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Novel Tools for Investigating Microglia Cellular Turnover Kinetics and Microglia-CD4 T Cell Interaction
… homeostatic functions in the brain. Increased MHCII expression in activated microglia further supports the hypothesis for T cell influence over microglia function during inflammation. Moreover, the change in microglia phenotype in the aged brain is coupled with CD4 T cell infiltration. This …
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Generation and analysis of transgenic mice expressing ovalbumin as a neo-self antigen under control of the myelin basic protein promoter
… of OVA specific MHCI restricted (OT-I) and MHCII restricted (OT-II) TCR Tg T cells extended the OVA Tg mouse model by allowing potentially encephalitogenic T cells to be tracked in vivo. Specificity for the target Ag should enable the dynamic role of antigen specific T cells in …
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Elucidating the Role of Antigen-presenting Cells in the Immunopathogenesis of the Porcine Reproductive and Respiratory Syndrome Virus
… protein gamma actin 1 associates closely with MHCII. When MoDCs were infected with PRRSV, gamma actin 1 was no longer associated with MHCII. We hypothesize that PRRSV could be manipulating the actin cytoskeleton, potentially interfering with MHCII peptide presentation. Ultimately, we were …
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Charakterisierung von Mikrogliafunktionen im Simianen Immundefizienzvirus (SIV) - Rhesusaffentiermodell für AIDS
… einzelner Oberflächenmoleküle, wie dem MHCII, korreliert mit der Viruslast in den Mikrogliazellen. Intrazellulär wurde die Transkription Interferon induzierter Gene und des antiretroviralen Faktors APOBEC3G im Gehirngewebe und in Mikrogliazellen mit Hilfe der Echtzeit PCR untersucht. …
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A role for polyunsaturated fatty acids in Th1-mediated disease
… the upregulation of co-stirnulatory markers and MHCII expression. Given the critical role of IL-12 and IL-10 in T helper cell differentiation we looked at the effects of PUFA-modified DC on subsequent T cell development and found them capable of inhibiting IFN-y, IL-17, IL-2, and IL-4 production …
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Glioblastoma: multifaceted immunosuppression mediated through galectin family members
… though insignificant, downregulation of MHCII and phagocytosis was observed in pro-inflammatory adult macrophages exposed to GBM-relevant galectin-1 concentrations. These results suggested a T-cell-specific mechanism of suppression resulting in reduced infiltration, whereas B-cells and …
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Selection Dynamics in the Germinal Center During Antibody Affinity Maturation
… through their BCRs for presentation as peptide-MHCII to CD4+ T cells. In turn, enhanced T cell help induces selected B cells to return to the DZ, where they spend a prolonged period of time proliferating in between rounds of competition in the LZ. By dividing a greater number of times during …
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Novel mechanisms of Atg16L1 recruitment in non-canonical autophagy
… lack of non canonical autophagy to a defect in MHCII antigen presentation. Furthermore this project has generated new refined Atg16L1 mutant models ablating non-canonical autophagy without affecting canonical autopahgy. In parallel, proteomic analysis was done to provide mechanistic insights …
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Investigations into the Biological Roles of the E3 ligase Ariadne 2/TRIAD1
… markers CD80/B7.1, CD86/B7.2, CD83, CD40 and MHCII and are capable of activating T cells in the RIP-GP model of induced diabetes. This may be linked to modulation of the NFκB and ERK pathways, in particular increase in nuclear p65/RelA and phospho-p65/RelA leading to an increase in NFκB and …
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Immunotherapeutic Strategies to Reverse Tumor Associated Immunosuppression in Breast Cancer
… of all four MDSC maturation markers, CD80, CD86, MHCII and F4/80. Additionally, Pam also significantly reduced the number of MDSCs in the treated whole splenocyte cultures.</p> <p>Future studies that are discussed here will expand our knowledge on the effect of tumor derived G-CSF in clinical …
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Molekulare Mechanismen der antiinflammatorischen und immunsuppressiven Effekte von Interleukin-10
… auf das für die Antigenpräsentation essentielle MHCII-Molekül HLA-DR nachgewiesen werden. Auch für die Vermittlung der stimulatorischen Wirkung von IL-10 auf den Fc?-Rezeptor CD64, der eine Rolle bei der Phagozytose spielt, konnte gezeigt werden, dass HDAC1 essentiell ist. Die Ergebnisse tragen …
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