Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 24 for “"MG1655"”.
-
Metabolic flux analysis of Escherichia coli MG1655 under octanoic acid (C8) stress
… By comparing the metabolic flux maps of E. coli MG1655 grown at different conditions, pathways that have flux change under stress were identified. Inhibition effect for several pathways, resulting in a reduction in carbon flux, was found under stress: the TCA cycle flux by ~ 44%; the malic enzyme …
-
An Investigation of the Genetic Control of Biofilm Formation in Bacteria (E. coli K-12 MG1655)
… cold, acid and hydrogen peroxide on E. coli K-12 MG1655 and its stress response in presence and absence of rpoS and bolA genes. E. coli cells were exposed to sub-lethal levels of each stress for 15 minutes in both planktonic and biofilm phases and post-stress response i.e. gene expression level …
-
Isolation and Characterization of Bacteriophages Targeting Uropathogenic E. coli
… phages using an E. coli laboratory strain; K-12 MG1655. Lytic phages against E. coli MG1655 were purified and host-range testing was conducted against a panel of 11 UPEC strains form the E. coli reference collection (ECOR). These lytic phages were characterized phenotypically using transmission …
-
Surface Functionalisation of Metal-Organic Frameworks for Biomedical Applications
… a DNA bridge was developed to selectively target MG1655 bacteria. PCN-222 was coated with three different nucleic acid coatings (DNA, DNAPhos, and PNAPhos), which were used to hybridise with the targeting complex. Among these, PNAPhos achieved the highest degree of colocalisation, as observed by …
-
Proteome, transcriptome and metabolome plasticity in closely related strains of Escherichia coli - K12 and its diversity during molecular evolution processes
… two closely related K-12 non-pathogenic strains, MG1655 and W3110 have been accurately determined. Resequencing of PCR products of selected regions indicates that there is only eight true insertion/deletion or base differences between the two strains in addition to the 13 sites where differences …
-
INVESTIGATION OF ANTIBIOFILM AND ANTIVIRULENCE ACTIVITY OF 5-FLUOROCYTOSINE IN ESCHERICHIA COLI
… activity to the E. coli laboratory strain MG1655 than 5-FU and 5-FUrd. However, all three fluoropyrimidines caused inhibition of curli production and general reduction of biofilm adhesion in dose-dependent manner in MG1655. This result was further confirmed by the observation that …
-
Error correcting optical mapping data
… accuracy on simulated E. coli (str. K-12 substr. MG1655) genome.
-
Molecular basis of NAIP/NLRC4 inflammasome activation by flagellin
… SL1344 and the non-pathogenic E. coli strain K12-MG1655. An important part of my work was to try and determine which regions of fliC are required for NAIP/NLRC4 inflammasome activation and whether they can be mutated while preserving motility. To do this a panel of ten strains expressing chimeric …
-
Using high-density mutagenesis to identify the genetic requirements for the growth of Escherichia coli
… genetic requirements for fitness of E. coli K-12 MG1655 during growth in the presence of bile and under anaerobic conditions in the presence of nitrate, representative of bile exposure in the duodenum and conditions of inflammation in the intestine. TraDIS reveals, in detail, genetic requirements …
-
Structure & Function of Bacterial Transport Machines in their Cellular Context
… mixed population of cells, by comparing parental MG1655 *Escherichia coli* with a cardiolipin deficient MG1655 strain. I found that the cardiolipin deficient strain has reduced T1SS levels compared to its parent. To aid structural studies, I optimise the expression of the T1SS using a flow …
-
Calcium homeostasis in Escherichia coli
… on global gene expression in wild-type cells (MG1655), (ii) employing transposon mutants (calcium-sensitive) and mutants that are defective in calcium transport, and (iii) performing quantitative analysis of a specific subset of 15 genes to elucidate their possible involvement in calcium …
-
A Platform for Microbial Evolution and Characterization
… are immune to washout. We have developed a new, MG1655 derived, strain of E. coli that does not form biofilms. The inability of our strain to form biofilms enables longterm evolution and characterization experiments in E. coli. Theory: We develop a stochastic hybrid systems model for evolution in …
-
Isolating and characterizing antimicrobial peptides derived from larvae of the blowfly Sarconesiopsis magellanica (diptera: Calliphoridae)
… having activity against Gram-negative (E. coli MG1655, P. aeruginosa ATCC 27853) and Gram-positive (S. aureus ATCC 29213, M. luteus A270) bacteria. The minimum inhibitory concentrations ranged from 1.2 μM upwards; the AMPs did not have toxicity in any tested cells and their action on bacterial …
-
Characterization of Bacterial Transcriptional Regulatory Networks through Genome-Wide in vitro Run-Off Transcription RNA-Seq (ROSE)
… ROSE was carried out on Escherichia coli K-12 MG1655 genomic DNA using the E. coli RNAP holoenzyme, which includes σ70. This procedure identified 3,226 transcription start sites, of which 2,167 were also found in in vivo studies and 598 were new. Many new promoters may be repressed under the …
-
Studying Mechanisms of Ionizing Radiation Resistance in Bacteria: Evidence for Multiple Pathways
… radiation (IR) resistant populations of E. coli MG1655 through twenty cycles of exposure to IR and outgrowth. To identify the genetic alterations responsible for increased IR tolerance, a total of seven isolates from the evolved final population were re-sequenced. However, the individual isolates …
-
Total proton flux and balancing in genome-scale models: The case for the updated model of Clostridium acetobutylicum ATCC 824
… to the iAF1260 genome-scale model of E. coli K12 MG1655 was explored. Relationships were found between: (1) the P/O ratio, (2) the SPF, (3) the growth rate, and (4) the production of acetate. As was expected, higher acetate production correlates with lower P/O ratios, while higher growth …
-
Role of <i>bax</i>, <i>ibpA</i>, <i>ibpB</i> and <i>cspH</i> Genes in Protecting CFT073 (Uropathogenic <i>Escherichia coli</I>) Against Salt and Urea Stress
… be well conserved among commensal <i>E.coli</i> MG1655 and 3 different UPEC strains CFT073, UTI89 and <i>E.coli</i> 536. Then GFP transcriptional fusion plasmids were constructed for <i>bax</i>, <i>ibpAB</i> and <i>cspH</i> genes and promoter activity assays were performed in K medium. Results …
-
Expression of the multi-drug efflux genes acrAB of Escherichia coli
… strains based on the lacZ derivative of E. co/i MG1655 (SD 1648) harbouring specific gene disruptions was constructed in order to investigate the effect of key regulatory factors on transcription of the acrAB genes. CRP-cAMP, FIS, H-NS, Rob, AcrR, OmpRJEnvZ, CsrAB, and SdiA were all found to have …
-
Microbial single-cell growth kinetics and heterogeneity in nutrient-limiting environments
… stoichiometry for glucose-growing *E. coli* MG1655 K12. For the first time, microfluidic growth kinetic parameters were shown to yield comparable values to conventional methods. <br /> This laid the foundation for determining the specific k_s values of the synthetic co-culture along with …
-
Response of intestinal Escherichia coli to dietary factors in the mouse intestine
… with the commensal Escherichia coli K-12 strain MG1655 were fed three different diets over three weeks: a diet rich in starch, a diet rich in non-digestible lactose and a diet rich in casein. Two dimensional gel electrophoresis and electrospray tandem mass spectrometry were applied to identify …
Page 1 of 2