Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 85 for “"MEFs"”.
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The Role of the Stress Response Gene Gadd45b in Senescence
… time that primary mouse embryo fibroblasts (MEFs) from Gadd45b null mice proliferate slowly; accumulate increased levels of DNA damage, and senesce prematurely. Notably, this is in contrast with Gadd45a null MEFs that show enhanced growth rate and escape senescence. This difference in growth …
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Altered DNA Repair, Antioxidant and Cellular Proliferation Status as Determinants of Susceptibility to Methylmercury Toxicity in Vitro
… 1-null (Ogg1-/-) murine embryonic fibroblasts (MEFs); and, (2) human Ogg1 (hOgg1)- or formamidopyrimidine glycosylase (Fpg)-expressing human embryonic kidney (HEK) cells; reciprocal in vitro cellular models with deficient and enhanced ability to repair oxidatively damaged DNA respectively. When …
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Direct Reprogramming Of Fibroblasts Into Muscle Or Neural Lineages By Using Single Transcription Factor With Or Without Myod Transactivation Domain
… reprogram the mouse embryonic fibroblasts (MEFs) into myogenic or neurogenic lineages, respectively. In addition, we created fusion transcription factors (Pax3 or NeuroD1) with the potent MyoD transactivation domain (MDA) that could facilitate radical acceleration of reprogramming into the …
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Disruption of Dna Polymerase Zeta Promotes An Innate Immune Response
… Immortalized <em>Rev3l</em>knockout (KO) MEFs are under considerable constitutive genomic stress, as demonstrated by elevated levels of micronuclei formation. As the cellular consequences of this stress remain unexplored, genome-wide transcriptional changes were measured in <em>Rev3l …
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The role of the mitofusins in adipose tissue
… and Mfn2-/-, mouse embryonic fibroblasts (MEFs) were differentiated into mature adipocytes. Despite the disruption of their mitochondrial morphology, Mfn1-/- MEFs manifested a marked increase in adipogenesis relative to WT or Mfn2-/- MEFs, as reflected by increased lipid accumulation, …
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Isolation and Characterization of Estrogen Receptor- Interacting Proteins
… from TRAP220-/- mouse embryo fibroblasts (MEFs) revealed that the interactions between TRAP and ERs are dependent on TRAP220, a TRAP/Mediator subunit previously shown to interact with ER and other nuclear receptors in a ligand-dependent manner. The physiological relevance of the in vitro …
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Characterization of serum-induced CYP1A1 expression and activity in mouse embryo fibroblasts
… TCDD. Using a TCDD dose-response treatment in MEFs, we observed a super induction of CYP1A1 with newborn calf serum (NCS) in the presence (10nM/15nM) of TCDD. In addition to NCS, fetal bovine serum (FBS) also has the capability to yield a CYP1A1 super induction. These results suggest that …
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Estrogen Regulation of Gene Expression and Analysis of the Role of the Coregulator, Repressor of Estrogen Receptor Activity (Rea)
… Studies using mouse embryo fibroblasts (MEFs) revealed that REA heterozygous MEFs displayed a greater transcriptional response to E2. These studies demonstrate that REA is a significant modulator of estrogen responsiveness in vivo.
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Role of Cyclin-Dependent Kinase Inhibitors in Estrogen Regulation of Adipogenesis
… Wild-type (WT) mouse embryonic fibroblast (MEFs) had increased mitotic clonal expansion (MCE) due to lack of estrogen but this was not seen in p27KO MEFs, that showed that the effects due to these two pathways were not additive and a possibility that they might be acting through the same …
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The function of E2F6 in the Polycomb complex
… E2f6'- mice and mouse embryonic fibroblasts (MEFs). The mutant mice are viable and survive into adulthood with similar lifespan as their littermate controls. Furthermore, the E2f6 null MEFs are indistinguishable from wild-type MEFs in asynchronous proliferation, cell cycle re-entry from …
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The Tumor Suppressor PKCδ Is Critical for UV-Induced G2/M Checkpoint Activation and Apoptosis
… DNA damage, we exposed wild type and PKCδ null MEFs to UV radiation. Wild type MEFs underwent a pronounced G2/M arrest, Cdk1 phosphorylation, and induction of apoptosis following UV exposure, while PKCδ null MEFs were resistant to these effects. Expression of PKCδ-GFP, but not caspase-resistant …
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Molecular Mechanisms of Programmed Necrotic Death Initiated by Intrinsic Death Signals
… knockout (DKO) mouse embryonic fibroblasts (MEFs) to study non-apoptotic death, we discovered DNA double strand breaks (DSBs) activate a genetic program that upregulates reactive oxygen species (ROS) and the lysosomal protease cathepsin to execute a form of "programmed necrotic death" (PND). …
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Mechanism of Action of Pentobarbital Induced Attenuation of Vascular Smooth Muscle Contraction
… 0.3 ms, 50 V, 30 sec), and direct smooth muscle (MEFS) stimulation in the presence of TTX (10 Hz, 3 ms, 50 V, 30 sec). Rings, preloaded at 1 g, were immersed in an organ bath filled with Krebs solution at 37°C, and aerated with 95% 02:5% CO<sub>2</sub>. PB (1 mM) attenuated only the tonic …
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Regulation of structure-selective nucleases by the SLX4 scaffold in ICL repair and HJ resolution
… with SLX4. Complementation studies in Mus81 null MEFs suggested that the role of MUS81-EME1 in ICL repair is independent of its interaction with SLX4. In contrast, the interaction of MUS81-EME1 with SLX4 is essential for ICL repair in two different human cell lines. Finally, I show that in human …
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Immortalisierung embryonaler Fibroblasten aus Wildtyp und Hupki (Human p53 knock-in) Mäusen durch Veränderungen des p19Arf/p53 Kontrollweges und Erst-Charakterisierung des neuen Prolin 72-kodierenden Hupki-Mausstamms
… den primären, embryonalen murinen Fibroblasten (MEFs) einen oxidativen Stress auf die Zellproliferation aus. P19/ARF-p53 scheint der wichtigste Regulationsmechanismus für die Seneszenz in diesen Zellen zu sein. Mit der Inaktivierung von Tumorsuppressorgenen wie z.B. p53 oder pRb durch …
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Realisation of De Gennes’ Absolute Superconducting Switch and Magnetic Exchange Field Effects at Metallic and Superconductor Interfaces
… are parallel-aligned (P), due to the addition of MEFs at both FI/S interfaces. Conversely, for antiparallel-aligned (AP) magnetisations, the superconducting state is recovered as the MEFs cancel each other out. While superconducting switches have been previously demonstrated, this thesis marks the …
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Sensing of Picornavirus Infections
… viruses differs in murine embryonic fibroblasts (MEFs) and HeLa cells in which there is efficient replication. In MEFs RIG-I was found to be essential for the expression of type I IFNs but contribute to increases in viral titers in response to CVB3 infections. MDA-5 inhibited CVB3 replication but …
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The Group IVA Cytosolic Phospholipase A2/C1P Interaction and Its Role in Eicosanoid Synthesis and Inflammation
… systems. We utilized primary mouse fibroblasts (MEFs) and macrophages isolated from CERK-/- and +/+ mice. The ceramide-1-phosphate and eicosanoid profiles were investigated, and both ceramide-1-phosphate and eicosanoid levels in CERK-/- MEFs were found to be dysregulated. This study also presents …
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Identification of novel proteins that regulate the amino acid-sensitive mTORC1 pathway
… mice and RagA-null mouse embryonic fibroblasts (MEFs). RagA is required for embryonic development, and, surprisingly, its deletion in adult mice leads to an expansion of monocytes. MEFs derived from RagA-null embryos display atypical, nutrient-insensitive mTORC1 activation. Finally, we identified …
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Gsk3 Mediates Signaling Upstream of Akt
… cancer cells and in mouse embryonic fibroblasts (MEFs) abrogated induction of Akt phosphorylation by the Akt catalytic domain inhibitor A674563. Our discovery that GSK3 can function upstream of Akt led us to explore the role of GSK3 in mediating signaling from growth factors to Akt. Knockdown of …
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