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Showing 1 to 9 of 9 for “"MEC1"”.
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Analyses of the MEC1 DNA Damage Pathway in Saccharomyces cerevisiae
… yeast, Saccharomyces cerevisiae, utilizes the MEC1 checkpoint pathway to regulate the DNA damage response. This study addressed two overarching themes of transcriptional and post-translational regulation within the MEC1 pathway. We first applied our understanding of the MEC1 DNA damage …
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ATR SIGNALING IN GENOME REPLICATION AND MAINTENANCE
… activates the sensor kinase ATR in mammals (Mec1 in S. cerevisiae), which coordinates a multifaceted cellular response that is essential for maintaining genome integrity. Over the last few decades, ATR’s role in controlling the checkpoint upon the DNA damage response has been well studied. …
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Affinity Maturation of the D1.3 Antibody Using Yeast Surface Display and Flow Cytometry
… produced a four-fold higher affinity mutant (MEC1). MEC1 was randomly mutagenized and screened by kinetic selection, identifying a large number of improved mutants. In both screens, library size was approximately 5 x 106, and mutagenesis was by error-prone PCR of the entire scFv gene. This …
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Molecular bases underlying chromosome fragility at Replication Slow Zones in Saccharomyces cerevisiae
… Inactivation of the essential checkpoint kinase, Mec1, in mec1-ts mutants results in replication fork stalling followed by chromosome breakage at RSZs. Interestingly, inhibition of ATR, the mammalian homologue of Mec1, also leads to chromosome instability at common fragile sites, suggesting that …
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HISTONE-DEPENDENT AND HISTONE-INDEPENDENT PATHWAYS FOR RAD9 CHROMATIN RECRUITMENT AND CHECKPOINT ACTIVATION
… and activation of the apical kinase complex Mec1-Ddc2 and for the loading onto DNA of the 9-1-1 complex. Once activated, Mec1 phosphorylates different targets, among which Ddc2, the Ddc1 component of the 9-1-1 complex, histone H2A. Another Mec1 target is adaptor Rad9. Phosphorylation of Rad9, …
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Zur Funktion des MPH1-Gens von Saccharomyces cerevisiae bei der rekombinativen Umgehung von replikationsarretierenden DNA-Schäden
… mit den DNA damage checkpoint Mutanten rad9 und mec1 wurden gefunden. Eine Modifikation von Mph1 durch Phosphorylierung ist wahrscheinlich.Trotz der Hypostasis von mph1 zu rad51, rad52 und rad55 sind mph1 Mutanten nicht defekt in der homologen Rekombination, vielmehr zeigen sie einen …
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Saccharomyces cerevisiae DNA helicases Mph1, Srs2 and Sgs1 collaborate for the reinitiation of stalled or collapsed replication forks
… an homologen DNA-Duplexen. Deletion der Gene MEC1, RAD53, RAD9, RAD17 und RAD24, die an der Checkpoint-Antwort auf DNA-Schäden beteiligt sind, kann ebenfalls zu einer Aufhebung des synthetischen Defektes führen. Darüber hinaus ist die rad54-Deletion sub-additiv zu mph1 in Bezug auf einen …
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Meiotic Dna Re-Replication And The Recombination Checkpoint
… However, the additional deletions of <em>RAD17, MEC1, MEK1, RED1, HOP1, or PCH2</em> restored the re-replication phenotype. These proteins are all individually required for the meiotic recombination checkpoint. Therefore, indicating that induction of the pachytene checkpoint by a gene deletion of …
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Regulation of FBP1 expression and changes in glucose metabolism are crucial for an appropriate response to DNA damage and aging in S.cerevisiae
… damage signalling cascade that starts with the Mec1/Tel1 damage sensors was investigated with the RNR2-GFP reporter assay. These experiments revealed that the deletion of FBP1 had no effect on induction through the RNR2 promoter while overexpression of FBP1 significantly increased the activity …