Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 28 for “"MDCK cells"”.
-
Role of matriptase in the regulation of epithelial barrier permeability studied using MDCK cells
… two!variants!of!MadinNDarby!canine!kidney!(MDCK)! cells!were! used,! together!with! the! “calciumNswitch”!model! of! epithelial! function! with!measurements!of!transepithelial!electrical!resistance!(TEER).! Inhibitors!of!matriptase!proteolytic!activity!delayed!the!restoration!of!TEER!after! …
-
Insulin-Like Growth Factor Binding Protein-6: Posttranslational modifications and sorting in polarized MDCK cells
… in der polarisierten Madin-Darby canine kidney (MDCK) Zelllinie. Die Daten ergaben, dass MDCK-Zellen verschiedene neutrale Proteasen sezernieren, die IGFBP-2 bis -5 in Fragmente definierter Größe spalten. IGFBP-6, das wichtigste sequestrierende Bindungsprotein für IGF II, wird unter transienter …
-
Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis
Membrane traffic in polarised cells was investigated by growing Madin-Darby canine kidney (MOCK) cells on ·permeable polycarbonate filter supports which allowed access to both sides of the cell monolayer. Membrane glycoconjugates on the apical and basolateral cell surfaces were labelled …
-
Purification of Vitamin D Binding Protein and Its Role in the Uptake of 25-Hydroxyvitamin D by Cultured Kidney Cells (Mdck Cells, Tissue Culture, Dbp)
… of 25(OH)D(,3) to study its uptake by kidney cells.
-
Localization of Human Prostaglandin E2 Receptors in Polarized Epithelial Cells
… of protein sorting in polarized epithelial cells are poorly understood. Several studies have determined membrane targeting of G protein-coupled receptors (GPCRs) using epithelial cells such as Madin-Darby canine kidney (MDCK) cells. Polarized epithelial cells are composed of apical and …
-
Organization and formation of the apical membrane of epithelial cells
… surface of untreated and raft lipid depleted MDCK cells with atomic force microscopy revealed that raft lipids are involved in the formation and/or maintenance of microvilli, actin based protrusions of the apical plasma membrane, indicating a regulatory link between membrane domains and the …
-
The biomechanical properties of epithelial cells and tissue in two and three dimensions
During development and disease progression, cells and tissue undergo mechanical changes and alter their response to external physical cues. This is also true during the onset of colorectal cancer, the second most common cause of cancer deaths in the Western world. Before the changes that occur …
-
Polarised secretion of leukaemia inhibitory factor
… a cytokine that is active on a wide variety of cells. Multiple LIF transcripts have been described. The transcripts LIF-D and LIF-M encode different signal peptides, which in mouse have been associated with differential localisation of the mature protein. LIF-D is associated with a freely …
-
Characterization of Molecular Glycerophospholipids by Quadrupole Time-of-Flight Mass Spectrometry
… composition of PCs of total lipid extracts of MDCK cells and of human red blood cells (RBC) could accurately be charted. Significant presence of positional isomers was observed increasing the total number of individual PC species close to one hundred. Secondly, the molecular PC and SM species …
-
Protein sorting to the apical membrane of epithelial cells
… proteins to the apical membrane of polarised cells. Based on this, the aim of this work was to contribute to the understanding of apical sorting in epithelial cells. The study of how lipid rafts are structured has been hampered by the scarcity of techniques for their purification. Rafts are …
-
Studies of fluorescent imaging for mRNA detection in living cells
… focuses on the study of imaging mRNA in living cells. To achieve this research objective, three approaches have been utilized:: 1) Imaging of a transgenic mRNA tagged by multiple repeats of malachite green: MG) binding aptamer.: 2) Imaging of inducible nitric oxide synthase: iNOS) mRNA by …
-
Analyzing Interactions Between Cells And Extracellular Matrix By Atomic Force Microscopy
Interactions of cells with the extracellular matrix (ECM) have important roles in various physiological and pathological processes, including tissue morphogenesis during embryonic development, wound healing and tumor invasion. Although most of the proteins involved in cell-ECM interactions have …
-
The role of sphingolipids in autosomal dominant polycystic kidney disease (ADPKD)
… or primary cilia in Madin-Darby Canine Kidney(MDCK) cells. Collectively, these data suggest that ST-066, ST-081, HWG-35D andPLR-11 affect signalling pathways of relevance to cyst growth; however, the potential application of SK2 inhibitors in ADPKD therapeutics remains to be determined.
-
Cytokine-bearing Influenza Vaccine: Adjuvant Potential of Membrane-bound Immunomodulators
… virions, produced from influenza infected MDCK cells expressing membrane-bound cytokines, readily incorporated membrane-bound cytokines during budding and these cytokines on the virus particles retained bioactivity following viral inactivation. In vivo vaccination studies in mice showed …
-
Human Cytochrome P450 3A4 Over-Expressing IEC-18 and MDCK Cell Lines as an In-Vitro Model to Assess Gut Permeability and the Enzyme Metabolism
… metabolism in the gut and the liver. Mammalian cells lines such as MDCK and Caco-2 are commonly employed to assess drug permeability but they lack or have low expression level of drug metabolism enzyme expression such as CYP3A4, which contributes to significant first-pass gut and liver …
-
Engineering Complex Kidney Structures for Disease Modelling, Drug Testing, and Studying Kidney Development
… sized and shaped scaffolds into which MDCK cells were seeded and cultured under tubulogenic conditions. Cells grew and self-assembled into branched tubules with single lumen delimited by a polarised monolayered epithelium, exhibiting kidney-specific functions. <br></br><br></br> To …
-
Investigating the mechanisms of cell competition in mammals using in vitro systems
… of a viable cell population, by fitter cells. Despite over forty years of research, the molecular mechanisms of competition in mammals are poorly understood. During my PhD I have investigated the mechanisms of competition by exploring an established mammalian cell culture system, in …
-
Protein kinase C isoform antagonism controls occludin phosphorylation and tight junction assembly
… induced the rapid phosphorylation of occludin in MDCK cells cultured in low extracellular calcium medium with a concomitant translocation of occludin to the regions of cell-cell contact. The extent of occludin phosphorylation, as well as the incorporation of occludin and ZO-1 into tight junctions, …
-
An Oxanthroquinone Derivative Disrupts Ras Plasma Membrane Localization and Function By Inhibition of Acylpeptide Hydrolase and Perturbation of Sphingomyelin Metabolism
… abrogated RAS-RAF-MAPK signaling in MDCK cells expressing constitutively activated, oncogenic mutant RASG12V. G01 also inhibited the proliferation of RAS-less mouse embryonic fibroblasts (MEFs) expressing oncogenic mutant KRASG12V or KRASG12D but not RAS-less MEFs expressing oncogenic …
-
Multifunctional Liposomal Carriers Encapsulating Temozolomide, RG7388 and Elacridar for Targeted Glioblastoma Multiforme Therapy
… that the liposomes showed lower toxicity in MDCK cells, while the toxicity for U87 and GBM 108 cells for the liposomal systems was higher than that of the drug solutions. Higher uptake for the folate decorated liposomes was seen in the U87 and GBM108 cell lines. The permeability of the …
Page 1 of 2