Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"MDA5"”.
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The Role of HOIL 1 Ubiquitin Ligase Activity in MDA5 Antiviral Signaling
The RIG-I-like receptors (RLRs), RIG-I and MDA5, are innate sensors of RNA virus infections that are critical for mounting a robust immune response. RIG-I and MDA5 both signal to MAVS to induce antiviral cytokine induction but respond to different viruses and are activated by distinct mechanisms. …
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Molecular Mechanism of MDA5 RNA Sensing in Innate Immunity and Disease Pathogenesis
… proinflammatory signal, and its recognition by MDA5 and its coregulatory factor, LGP2, is essential for initiating antiviral responses. This study aimed to investigate the structure and function of MDA5 and LGP2. I showed the cooperative nature of ATP hydrolysis in MDA5-dsRNA filaments. I …
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The Role of MDA5 and TLR3 in Response to dsRNA and Viral Infection
… TRIF. Melanoma differentiation-induced gene 5: MDA5) is a member of the RLR family that recognizes dsRNA within the cytosolic compartment and signals through the adaptor IPS-1. Although TLR3 and MDA5 initially employ distinct downstream adaptors, both are known to induce the production of …
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Evasion of RIG-I/MDA5 and TLR3-mediated innate immunity by hepatitis A virus
… extensive research. Cellular helicases RIG-I and MDA5, and Toll-like receptor 3 (TLR3) are PRRs that detect virus-specific double stranded RNA (dsRNA). Activation of these PRRs by dsRNA lead to their interaction with adaptor proteins, which engage downstream kinases to activate two critical …
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The essential, nonredundant roles of RIG-I and MDA5 in detecting and controlling West Nile virus infection
… virus, depends on recognition by RIG-I and MDA5, cytosolic pattern recognition receptors (PRRs) of the RIG-I-like receptor (RLR) protein family that recognize viral RNA and activate defense programs that suppress infection. We evaluated the individual functions of RIG-I and MDA5 both in …
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The regulation and function of viperin during rhinovirus infection
… melanoma differentiation-associated protein 5 (MDA5), or IL-29 signalling, promotes IRF-1 mediated viperin transcription. Viperin then establishes a protein-protein interaction with MDA5 which results in enhanced MDA5 stability in RV infected cells. This leads to enhanced signalling through …
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Elucidating Mechanisms of Immunity to Nipah Virus Infection through Generation of Attenuated Viruses and a Single-Cycle Vectored Vaccine
… melanoma differentiation-associated protein 5 (MDA5) and attenuated virulence in an otherwise lethal ferret model. The NiV V-MDA5 binding site and the effects of blocking this interaction on virulence were previously unknown. The work described here identified amino acid I414 in NiV V as a …
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Mechanism of Ubiquitin Induced Activation of RIG-I Like Receptors in Antiviral Innate Immunity
… of the RIG-I like receptor family, RIG-I and MDA5, are essential for the detection of viral RNA in the cytoplasm. Upon viral recognition, RIG-I and MDA5 activate the mitochondrial signaling protein AVS, and initiate downstream signaling pathways that activate the transcription factors …
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Identification and Characterization of Auxiliary Factors in Innate Antiviral Immunity
… for eliciting the host intrinsic immune defense. MDA5 and RIG-I are two major cytosolic viral RNA sensors that recognize different viruses and initiate downstream signaling leading to the induction of type I IFNs antiviral responses in a majority of cell types. Mounting evidence suggests that …
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Key Role of Lys63-Linked Polyubiquitination in Viral Activation of IRF3
… RIG-I-like receptors (RLRs), including RIG-I, MDA5 and LGP2, are responsible for sensing intracellular viral RNAs. MAVS, a mitochondria-localized transmembrane protein, transduces signaling from RIG-I and MDA5 to activate downstream transcription factors IRF3 and NF-kB, which contribute to the …
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The Adar-Mavs Pathway Is A Critical Mediator of The Innate Immune System In Pancreatic Development and Cancer
… to prevent recognition of endogenous RNA by MDA5, a cytosolic RNA sensor. Activation of MDA5 by viral RNA can stimulate the innate immune system. Thus, ADAR-mediated RNA editing is crucial to distinguish “self” from “non-self”. ADAR has an important role in gene regulation as A-to-I editing …
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Pichinde virus infection of outbred Hartley guinea pigs and mice as small animal models of human Lassa fever
… genetic ablation of the host proteins RIG-I and MDA5. This study was carried out to determine the importance of RIG-I and MDA5 in providing innate immune protection against Pichinde virus in mice, which may shed important light on virus-host interactions in the natural rodent reservoirs of Lassa …
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Molecular insights into modulation of host innate immune response by viral proteins of RNA viruses SARS-CoV, HCV and Influenza virus
… molecular patterns (PAMPs). TLR3, RIG-I and MDA5, trigger complex intertwined signaling pathways in response to viral dsRNA leading to the activation of interferon regulatory transcription factors IRF3, IRF7 and NF-κB. These transcription factors mediate inflammatory process to clear …
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Hepatitis C Virus NS5A Targets the Nucleosome Assembly Protein NAP1L1 to Control the Interferon Response
… and NF-κB levels. Hence, both the TLR3 and RIG-I/MDA5 pathways are affected by NAP1L1 depletion. I could further demonstrate that NAP1L1 controls the basal transcription of genes involved in the immune pathway and that it interacts with the adaptor protein MAVS, which is required for RIG-I/MDA5 …
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The Genetic, Molecular, And Cellular Bases Of Unidentified Primary Immunodeficiencies
… cytosolic pattern recognition receptor of dsRNA MDA5, in the third family of study. The affected individual presented with recurrent severe respiratory infections with RNA viruses including human rhinovirus, coronaviruses (HKU1, OC43, NL63), influenza virus, and respiratory syncytial virus. The …
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Inosine-containing mRNA induces an innate immune response and is translated with lower efficiency
… it cannot be attributed to the sensors RIG-I, MDA5, TLR3, or PKR.
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In vitro effects of different innate-immune stimulants on Chinook salmon embryo cell line (CHSE-214): transcriptional and translational studies
… innate effectors: cathelicidin, hepcidin, iNOS, MDA5, RIG-I and ISG25 mRNA was assessed by qPCR in Chinook salmon cell line (CHSE-214) before and after treatment with potential inducers: ß-hydroxy-ß-methylbutyrate, 4-phenylbutyrate, 1,25(OH)2 D3, omega-3 and omega-3 ethyl ester from fish oil and …
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Understanding The Role of Arglu1 In Interferon Signaling Activation In Breast Cancer
… recognized by dsRNA sensors such as RIG-I and MDA5, leading to augmented IFN signaling. To identify RNAs directly bound by ARGLU1, Halotag-fused ARGLU1 was expressed in MCF7 cells for conducting CLIP-seq. These results elucidate unappreciated regulators of type I IFN signaling in breast cancer, …
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Comparative Analysis of Antiviral Responses in Mouse Embryonic Stem Cells and in Mesenchymal Stem Cells
… Our results also showed that TLR3, RIG! and MDA5, the receptors for viral RNA, are expressed at lower levels in mouse ESCs (mESCs) than in C3H10Tl/2 cells. However, mESCs are susceptible to LACV infection resulting in cell death, which can be reduced by IFNP pretreatment. Furthermore, IFNP …
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Visualisation of innate immune signalling complexes in cells by cryo-electron tomography
… in cells. RIG-I-like receptors (RLRs), RIG-I, MDA5 and LGP2 sense cytosolic viral dsRNA and then promote oligomerisation of the mitochondrial antiviral- signalling protein (MAVS) on the outer mitochondrial membrane. MAVS signalling induces activation of cytosolic kinases, which in turn …
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