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Showing 1 to 20 of 44 for “"MCM2"”.
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The Roles of the Mcm2-7 Tails in Replication Initiation
… when two copies of the replicative helicase, the Mcm2-7 complex, are loaded onto DNA. However, these helicases are loaded in an inactive state, and are not activated until the following S phase. As cells enter S phase, the action of two kinases, DDK and S-CDK, facilitates the recruitment of two …
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Mechanism and importance of Mcm2-7 double-hexamer formation during DNA replication initiation
… begins with loading of two heterohexameric Mcm2-7 helicases around origin DNA during G1 phase. The two helicases are loaded in opposite orientations and interact with each other at their N-terminal domains to form a head-to-head "double hexamer". In S phase, the helicases are activated by …
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An ORC Flip Enables Bidirectional Helicase Loading
… of all potential origins by loading two inactive Mcm2-7 replicative DNA helicases around the origin DNA in a head-to-head double hexamer. This conformation prepares the helicases to initiate bidirectional replication upon entry into S-phase. Interactions between three proteins and the Mcm2-7 …
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The role of Orc6 in ORC binding-site switching during helicase loading
… complex (ORC) coordinates loading of two Mcm2-7 hexameric helicases in opposite orientations to form the Mcm2-7 double hexamer. These Mcm2-7 double hexamers mark sites of eventual bidirectional replication initiation upon entering S-phase, when activation of these helicases and recruitment …
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Multiple roles of the replication initiation protein Cdtl during helicase loading in S. cerevisiae
… replicative DNA helicase, the hetero-hexameric Mcm2-7 complex. As cells enter G1 phase of the cell cycle, all potential sites of replication initiation are selected by the loading of inactive Mcm2-7 double hexamers. The anti-parallel orientation of the Mcm2-7 hexamers within the double hexamer …
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Mechanism of Mcm10 function during DNA replication
… of this process is the replicative DNA helicase, Mcm2-7. Mcm2-7 complexes are loaded around origins of DNA replication during G1 phase. As cells transition from G1 to S phase, the Mcm2-7 helicases are activated resulting in the recruitment of DNA polymerases and accessory proteins to begin DNA …
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Changing protein-DNA interactions promote ORC binding site exchange during replication origin licensing
… process is the eukaryotic replicative helicase, Mcm2-7, which leads the replication process by unwinding the DNA strands at the front of the replication machinery. To prepare for bidirectional replication, the hexameric Mcm2-7 helicases must be loaded onto origins of replication as head-to-head …
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Treslin and its role in the assembly of the replicative DNA helicase
… replisome is made up of six-subunit protein ring Mcm2–7, which contains weak DNA helicase activity. In order to fully activate the helicase activity, protein co-activators of Cdc45 and GINS have to be recruited to assemble a replicative DNA helicase called the CMG (Cdc45-Mcm2–7-GINS) complex. The …
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Single-molecule studies of the mechanism of eukaryotic helicase activation
… events that center the replicative DNA helicase Mcm2-7: helicase loading and helicase activation. During G1 phase, two inactive Mcm2-7 are loaded unto origin DNA. Upon entry into S phase, the association of multiple factors will promote helicase activity. Although loaded helicases mark all …
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Impact of DNA accessibility and sequence on eukaryotic origin licensing
… two hexamers of the eukaryotic helicase, Mcm2-7, are loaded at potential origins of replication in a head-to-head orientation. They remain together, inactive, until entry into S phase. Origin licensing depends on three proteins, the origin-recognition complex (ORC), Cdc6, and Cdtl, as well …
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Keep the ORCs at bay : how eukaryotic cells ensure one round of DNA replication per cell cycle
… three of the four helicase-loading proteins: Mcm2-7, Cdc6, and the Origin Recognition Complex (ORC). Phosphorylation of free Mcm2-7 and Cdc6 leads to their removal from the nucleus (Mcm2-7 by nuclear export and Cdc6 by protein degradation). However, phosphorylated ORC remains in the nucleus …
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Analysis of cyclin-CDK mediated regulation of the G1/S transition in post quiescent murine fibroblasts
… cells. CDK inhibition reduced levels of CDC6, MCM2 and PCNA in all contexts. Evaluation of the role of individual cyclin-CDK complexes was performed by siRNA mediated depletion. Cyclin E1/E2 depletion showed that Cyclin E regulates CDC6, MCM2 and PCNA at the transcriptional level; whereas …
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The role of DNA sequence during helicase loading at S. cerevisiae origins of replication
… cells enter the GI phase of the cell cycle, the Mcm2-7 replicative helicase is loaded at all potential origins of replication but remains inactive until S phase. Although the proteins involved in helicase loading have been elucidated, little is understood about the role of origin DNA sequence in …
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Phosphorylation of the replicative helicase by the S-phase kinase, Dbf4-Cdc7, at origins of replication in Saccharomyces cerevisiae
… several proteins to the DNA, including the Mcm2-7 replicative helicase, to form the pre-Replicative Complex (pre-RC). Origins are activated by recruitment of other initiation factors to the pre-RC to form the pre-Initiation Complex (pre-IC) and this event requires two kinases, Cyclin …
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Functional dissection of the S. cerevisiae helicase activating factor Cdc45
… During the G1 to S transition, loaded Mcm2-7 helicases are activated by the S-CDK (S phase cyclin-dependent kinase)- and DDK (Dbf4-dependent kinase)- dependent recruitment of the helicase activating proteins Cdc4S and GINS. The resulting Cdc45/Mcm2-7/GINS (CMG) complex unwinds …
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Investigating the Mechanism of Geminin Inactivation
… loading of an essential replication factor, the MCM2-7 helicase. This is loaded onto DNA early in the cell cycle. MCM2-7 stay bound on chromatin until cells enter S-phase where CDKs act upon the MCM2-7 complex leading to the initiation of DNA replication. The processes of replication licensing …
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A study of the relationship of the internal endocrine environment to the p53 network, cyclin D1 and replication licensing proteins in breast cancer
… in expression of various tumour markers (p53, mcm2 and cyclin D1) with change in internal endocrine environment in patients with primary breast cancer and to study the expression and prognostic association of cyclin D1 and replication licensing proteins (mcm2 and geminin) in invasive breast …
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Investigation of Cdc45 function during helicase activation
… DNA replication requires the stepwise loading of Mcm2-7 helicase complexes at origins of replication. These loaded helicases need to be activated during DNA replication initiation to unwind the DNA double helix and provide a ssDNA template for replicative DNA polymerases. Eukaryotic cells …
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Funktionelle Analyse der Interaktion und Lokalisation von Replikationsfaktoren und replikationsrelevanten Proteinen in Mauszellen
… mit den Replikationsproteinen ORC2, ORC6, MCM2, MCM3, MCM6 und CDC45 im Two Hybrid-System und Interaktionen mit MCM2 und MCM3 in Biolumineszenz-Resonanzenergietransfer-Experimenten auf. Eine Kolokalisation von Pescadillo und ORC6 in den Nukleoli läßt auf eine Funktion beider Proteinen bei …
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Charakterisierung des humanen Proteins MCM8 und seiner Interaktion mit CDC45
… Rolle. Ein heterohexamerer Komplex der Proteine MCM2-7 stellt in eukaryotischen Zellen die replikative Helikase dar, welche den DNA-Doppelstrang entwindet und so für die Replikation durch die DNA-Polymerasen zugänglich macht. In den Archaea übernimmt diese Aufgabe ein homohexamerer MCM-Komplex. …
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