Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 36 for “"MCF7 cells"”.
-
Mitochondrial Function as a Tool for Assessing Function, Quality and Adulteration in Medicinal Herbal Teas
… and commercial teas. Methods MCF-7 and MCF-10A cells were seeded and treated for 24 hours with an original liquorice tea extract (OLTE; Three Licorice Tea; Pukka, UK) and subjected to mitochondrial functional assays. Use of pre-extracted material was selected as a method of functional …
-
THE ROLE OF RETROTRANSPOSITION AND EXOSOMAL DNA IN RADIATION-INDUCED GENOMIC INSTABILITY
… formation in the descendants of irradiated cells many generations after the initial radiation insult. At present the mechanism of RIGI remains elusive, however it is possible that the random insertion nature of the LINE-1 retrotransposons could be a cause of RIGI. The work in this thesis has …
-
Synthesis of Novel CYP1 Activated Heterocyclic Anticancer Prodrugs
… have been found to be over-expressed in tumour cells whilst undetected or present in very low levels in corresponding normal tissue. This presented a novel target for the development of anti-cancer prodrugs, which would remain non-toxic until undergoing metabolism to toxic species by CYP1 …
-
Effects of 5-fluorouracil Drug Treatment on the Expression Profile of MicroRNAs in MCF7 Breast Cancer Cells
… studied the global effects of 5-FU treatment on MCF7 breast cancer cells. The dose-response curve obtained after the treatment of MCF7 cells with 23 different 5-FU concentrations for 48 hours showed an atypical bimodal or biphasic curve, thus indicating a plausible dual mechanism of action for …
-
Trim28 Interacts With Ezh2 to Regulate Its Activity
… EZH2 and regulates its activity in breast cancer cells. EZH2, the major H3K27 tri-methyltransferase, is over expressed in multiple subtypes of breast cancer; however, whether EZH2 plays an active role in breast cancer progression or treatment resistance is unknown. Here, we determined …
-
Estrogen sulfotransferase (sult1e1) expression and function in mcf10a-series breast epithelial cells: role as a modifier of breast carcinogenesis and regulation by proliferation state
… cancer progression and in ERα-positive luminal MCF7 breast cancer cells. Low levels of ERα and ERβ mRNA were present in MCF10A-derived cell lines. SULT1E1 mRNA was more abundant in confluent relative to subconfluent MCF10A cells, a non-tumorigenic proliferative breast disease cell line. SULT1E1 …
-
The role of orphan nuclear receptor DAX-1 (NR0B1) in human breast cancer cells: expression, proliferation and metastasis
… cell proliferation and metastasis, we used MCF7 breast cancer cells and MCF10A normal breast cells and manipulated their DAX-1 expression to increase DAX-1 expression by adenovirus infection in MCF7 cells, or knockdown expression of DAX-1 through the use of RNAi in MCF10A cells. We found a …
-
The role of RNAs and extracellular vesicles in the radiation response
The bystander effect (BE) occurs when cells that have not been directly traversed by ionizing radiation exhibit DNA damage effects as though they had been. It is established that signalling molecules such as cytokines and reactive oxygen species (ROS) signal to surrounding cells to mediate BE, …
-
Regulation of SPARC Gene Expression by the Activator Protein 1 Transcription Factor
<p>Overexpression of the c-Jun proto-oncogene in MCF7 breast cancer cells results in a variety of phenotypic changes related to malignant progression including a shift to estrogen independent growth, increased cell motility and invasion. Concurrent with these phenotypic changes are alterations to …
-
Role of DDX RNA helicases in cancer cells
… Cannizzaro ROLE OF DDX RNA HELICASES IN CANCER CELLS Abstract DEAD-BOX (DDX) RNA helicases are a large family of proteins characterized by the presence of a DEAD/H (Asp-Glu-Ala-Asp/His) motif. Their main function is to unwind double stranded RNA to promote downstream molecular events. They are …
-
Proteomic characterisation of Estrogen Receptor (ERα) interactome in breast cancer
… temporal changes of the ERα interactome in MCF7 cells upon OHT treatment. Our pipeline was also successfully applied for the study of other factors, such as CBP, NCOA3 and RNA Polymerase II. The integration of the different interactome datasets, lead to the discovery of a transcription …
-
Effect of phytoestrogens on breast and prostate cancer cell lines – potential implications for bone metastasis
… modifying effect of cytokines that tumour cells are exposed to in the bone micro environment will also be studied including TGF-β, BMP7 and IL-33. Results showed that phytoestrogens genistein and daidzein significantly reduced prostate cancer cell viability (PC3 cells) with concentration …
-
Regulation of RUNX3 in gastric and breast cancer
… product CagA is injected into gastric epithelial cells and disturbs cellular functions by physically interacting with and deregulating a variety of cellular signaling molecules. RUNX3 is expressed gastric epithelial tissues, and is frequently inactivated in gastric cancer. In the first part of the …
-
Radiation-induced genomic instability and cellular communication: mechanistic investigations
… between irradiated and un-irradiated (bystander) cells can cause damage in cells that are not directly targeted by ionizing radiation (IR); a process known as the bystander effect (BE). BE can also lead to genomic instability (GI) within the progeny of bystander cells, similar to the progeny of …
-
Trim24-Regulated Estrogen Response Is Dependent On Specific Histone Modifications In Breast Cancer Cells
… in tumorigenic human breast cancer-derived MCF7 cells. In the first</p> <p>part, I proved that TRIM24-PHD finger domain, which recognizes unmethylated</p> <p>histone H3 lysine K4 (H3K4me0), is critical for ERα-regulated transcription.</p> <p>Therefore, when LSD1-mediated demethylation of …
-
The anti-cancer activity of a novel palladacycle, BTC2, in oestrogen receptor positive and triple negative breast cancers
… and oestrogen receptor-positive breast cancer cells. However, AJ-5 is poorly soluble, therefore, a series of water soluble AJ-5-based compounds were synthesized. The aim of this study was to test and characterise the anti-cancer activity of one of these AJ-5 analogues, BTC2, in triple negative …
-
Translational control mechanisms in the p53 response network
… treatment-specific translatome profiling in MCF7 cells upon Doxorubicin and Nutlin-3a treatments. Among translated genes, we detected the presence of translationally enhanced mRNAs with a virtually absent transcriptional modulation; those genes were enriched for apoptotic functions, …
-
Understanding The Role of Arglu1 In Interferon Signaling Activation In Breast Cancer
… type I interferon signaling in breast cancer cells. This phenomenon was also seen in mouse breast cancer cells but was not seen in normal breast epithelial cells. Furthermore, my results suggested that the loss of ARGLU1 induced accumulation of dsRNAs, which were recognized by dsRNA sensors …
-
Chronic cadmium exposure alters ERα dependency and drug sensitivity of breast cancer cells
… genes and increased the malignancy of MCF7 breast cancer cells. Although these studies support cadmium’s function as a hormone disrupter, the role of ERα in cadmium-induced breast cancer progression remains unclear. Therefore, the goal of this thesis is to better understand how chronic …
-
ROLE OF AUTOPHAGY IN RADIOSENSITIZATION OF BREAST TUMOR CELLS
In MCF-7 breast tumor cells, ionizing radiation promoted autophagy that was cytoprotective; pharmacological or genetic interference with autophagy induced by radiation resulted in growth suppression and/or cell killing (primarily by apoptosis). The hormonally active form of vitamin D, 1,25D3, also …
Page 1 of 2