Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"MCF10A"”.
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Estrogen sulfotransferase (sult1e1) expression and function in mcf10a-series breast epithelial cells: role as a modifier of breast carcinogenesis and regulation by proliferation state
… and catechol-O-methyltransferase (COMT) in an MCF10A-derived lineage cell culture model for basal-like human breast cancer progression and in ERα-positive luminal MCF7 breast cancer cells. Low levels of ERα and ERβ mRNA were present in MCF10A-derived cell lines. SULT1E1 mRNA was more abundant …
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The role of Biophotons in cellular senescence.
… and A549 lung) and two non-cancer cell lines (MCF10A breast and IMR-90 lung fibroblasts). Dox-treated cells showed senescentrelated physiological changes, including decreased proliferation (P ≤ 0.05), increased betagalactosidase activity (P ≤ 0.001), increased ROS (P ≤ 0.01), mitochondrial …
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BREAST CANCER PROGRESSION AND METASTASIS: DISCOVERY OF NOVEL GENES AND THEIR FUNCTIONAL STUDY
… gene expression analysis of syngeneic cell lines MCF10A, DCIS.com and MCF10CA and cross-referenced the targets using patient cohorts. By comparing gene expression between MCF10A and DCIS.com cell line, we identified ID2 as a regulator of DCIS initiation. ID2 promoted DCIS formation by enhancing …
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Identification of Cell Biomechanical Signatures Using Three Dimensional Isotropic Microstructures
… model included HS68 normal human fibroblasts, MCF10A normal human breast epithelial cells and MDA-MB-231 metastatic human breast cancer cells. Primary findings on the first generation of silicon substrates demonstrated a distinct adhesion and growth behavior in HS68 and MDA-MB-231 cells. …
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DAPK3 Suppresses Mammary Acini Morphogenesis and is Required for Mouse Development
… understanding of DAPK3 we</p><p>utilized the MCF10A 3D tumorigenesis model in vitro and generated a constitutive DAPK3 knockout mouse. Using the MCF10A 3D morphogenesis model we identified that loss of DAPK3 through lenti-viral mediated knockdown accelerates MCF10A acini proliferation and …
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The role of PTEN as a PI(3,4)P2 lipid phosphatase in Class I phosphoinositide 3-kinase signalling
… pathways in human mammary epithelial cells (Mcf10a) stimulated with epidermal growth factor (EGF). By means of genetic suppression of PTEN and INPP4B, we revealed an unexpectedly high level of PI(3,4)P2 that accumulates in EGF-stimulated PTEN-INPP4B-KO Mcf10a cells. Further, an in vitro …
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Mitochondrial Function as a Tool for Assessing Function, Quality and Adulteration in Medicinal Herbal Teas
… 72.6% (p < 0.01) and 85.16% (p < 0.01) in MCF10A cells treated with 500 and 2000 μg / mL OLTE respectively. The effects of mitochondrial modulating drugs were reduced in MCF7 cells pre-treated with 2000 μg / mL OLTE (oligomycin: 29.94%, p < 0.01; FCCP: 43.2%, p < 0.01; antimycin A & …
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Analysis of gene expression alterations in premalignant progression from normal mammary epithelium to ductal carcinoma in situ by multiple orthogonal tools
… in comparison to normal breast epithelial cells (MCF10A) that may enable us to identify novel markers of disease progression and potential therapeutic targets. We cultured the cells in three dimensional reconstituted basement membrane (3D rBM) for RNA extraction and used the purified RNA for whole …
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Exploiting Clausius-Mossotti Factor to Isolate Stages of Human Breast Cancer Cells: Theory and Experiment
… investigated numerically and experimentally. MCF10A, MCF7, and MDA-MB231 human breast cancer cells were used to represent early, intermediate, and late staged breast cancer respectively. Experiments were conducted at 0.02ml/hr with applied voltages of 20Vrms, 25Vrms, 30Vrms, 35Vrms, 40Vrms and …
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Trim24 Orchestrates Metabolic Reprogramming and Emt In Breast Cancer
… cells.</p> <p>Upon TRIM24 over-expression in MCF10A cells, a mesenchymal-like change in the cellular morphology was observed. TRIM24-MCF10A cells exhibited marked decrease of E-cadherin and acquisition of various mesenchymal markers such as N-cadherin, fibronectin and vimentin. Similarly, upon …
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Amphiregulin (areg) And Epidermal Growth Factor (egf): Disparate In Egfr Signaling And Trafficking
… from cDNA transcribed from RNA isolated from MCF10A cells growing in the presence of AREG or EGF and after 24 hours withdrawl of the respective ligand. Genes regulating WNT signaling, but not NOTCH signaling, were altered in the MCF10A cells. Thus, the pathway that AREG/EGFR signaling effects …
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The role of orphan nuclear receptor DAX-1 (NR0B1) in human breast cancer cells: expression, proliferation and metastasis
… metastasis, we used MCF7 breast cancer cells and MCF10A normal breast cells and manipulated their DAX-1 expression to increase DAX-1 expression by adenovirus infection in MCF7 cells, or knockdown expression of DAX-1 through the use of RNAi in MCF10A cells. We found a trend toward increased cell …
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ROLES OF LIPOGENESIS IN BREAST CANCER PROGRESSION
… We observed that normal breast epithelial cells MCF10A cells have very low level of FAS expression whereas breast cancer cell lines MCF7, MDA MB231 and MDA MB231 LM have significant overexpression. Next, we observed the similar trend of FAS overexpression in breast cancer stem-like cells (CSCs) …
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Synthesis of Novel CYP1 Activated Heterocyclic Anticancer Prodrugs
… 468, MCF7 and MDA 231 cell lines. The non-tumour MCF10A cell line which has no basal CYP1 expression was used as the control. A library of eighteen 3,5-diarylpyrazoles were synthesised. The lead pyrazole DMU 10107 (3-(2,3,4-trimethoxyphenyl)-5-(3,4-methylenedioxyphenyl)pyrazole) gave an IC50 value …
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ROLE OF THE ENDOCYTIC PROTEIN EPSIN 3 IN MAMMARY GLAND TRANSFORMATION
… in non-transformed mammary epithelial cells (MCF10A), we showed that it induces a partial epithelial-to-mesenchymal transition (pEMT), a state linked to the expansion of the stem cell pool and to cellular invasion. Mechanistically, EPN3 overexpression induces pEMT by enhancing E-Cadherin …
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Use of fourier transform- infrared spectroscopic imaging as a tool for understanding early molecular events driving breast cancer progression
… breast epithelial cells, and co-culture with MCF10A normal breast epithelial cells in trans-well co-culture and also three-dimensional cell culture. α-SMA expression was determined using immunofluorescence and the samples were surveyed in a holistic and label-free approach using chemical …
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ROLE OF AUTOPHAGY IN RADIOSENSITIZATION OF BREAST TUMOR CELLS
… of autophagy did not sensitize noncancerous MCF10a cells to radiation; conversely, 4T1 mouse mammary tumors were highly sensitive to pharmacological inhibition of autophagy, suggesting selective radiosensitization against cancer cell lines. The current studies are consistent with the premise …
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ESTABLISHING PTERIDINE METABOLISM IN A BREAST CANCER CELL MODEL AND QUANTIFICATION OF SILVER NANOPARTICLE INTERACTIONS WITH YEAST CELLS USING MASS SPECTROMETRY
… pteridine metabolism in an isogenic progressive MCF10A breast cancer cell model. The folate-derived pteridine pathway in breast cells was established by individually dosing cell cultures with folic acid and 15 pteridines. Eight potential pteridine biomarkers in breast cancer cells were identified …
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