Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 94 for “"MCF-7 cells"”.
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COMPARATIVE PROTEOMICS STUDIES OF SOLUBLE NUCLEAR PROTEINS OF DRUG SUSCEPTIBLE AND RESISTANT HUMAN BREAST CANCER MCF-7 CELLS
… anticancer drugs target the nuclei of the cancer cells, differential expression of nuclear proteins may play crucial roles as cancer cells acquire drug resistance. Thus I have carried out a comparative proteomics research project to study differential expression of nuclear proteins in drug …
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Investigating the Involvement of RNA-Binding Protein ZFP36L1 in Breast Cancer Progression
… CRISPR Cas9-derived knockout of ZFP36L1 in the MCF-7 breast cancer cell line, characterised by luminal A molecular subtype. Functional assays demonstrated that the loss of ZFP36L1 in MCF-7 cells reduced the cell migration capacity and reduced cell population in the G1 phase of the cell cycle. …
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Mechanisms of 17-beta-estradiol regulation of the proto-oncogene Bcl-2 in MCF-7 human breast cancer cells.
… constructs stably transfected into MCF-7 cells. An approximately 1.7 kilobase (kb) sequence which directed estrogen-dependent regulation of Bcl-2 expression in these stable MCF-7 clones was identified. Another agent which may play a role in the regulation of Bcl-2 expression is the …
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Estrogen Inducible Serpin, Proteinase Inhibitor 9, in Immunosurveillance and Apoptosis
… attracted a great deal of recent interest. In MCF-7 cells, we find that 10 nM genistein induces PI-9 as effectively as saturating E2, and genistein treated MCF-7 cells are resistant to NK cell mediated cytotoxicity. In ERalphaHA cells, the dose response curves for induction of PI-9 by E2 and by …
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Antitumor activity of antimalarials in human breast cancer cells
Previously we showed that quinidine arrested MCF-7 cells in G1 phase of the cell cycle and led to a G1 to G0 transition followed by apoptotic cell death (Wang et al., 1998). The present experiments demonstrate that MCF-7, MCF-7ras, T47D, MDA-MB-231, and MDA-MB-435 cells transiently differentiate …
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Mechanism of Action of Antiestrogens in Breast Cancer and Uterine Cells (Estrogen Receptor)
… synthesis were studied in human breast cancer cells and rat uterus. Antiestrogens bind with high affinity to the estrogen receptor (ER) and to additional microsomal binding sites to which estrogens do not bind called antiestrogen binding sites (AEBS). Studies with the triphenylethylene AE, …
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Proteomic Map of ER+ Breast Cancer Cell Cycle
… cycle resulting in abnormal proliferation of cells that can bypass tightly regulated molecular checkpoints. Breast cancer is the most common cancer diagnosed in women, ~70% of cases displaying an estrogen receptor positive (ER+) phenotype. The aim of the present work was to generate a …
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Ursolic acid and oleanolic acid as novel therapeutic agents in breast cancer
… in breast cancer were studied in vitro in MCF-7 breast cancer cells and MCF10A breast cells. In this study the first aim was to establish the influence of OA and UA on cell growth and to see if opposing proliferation patterns could observed between the presumably ERɑ negative (ERɑ/ß - /+) …
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Characterization of the molecular mechanisms underlying retinoic acid induced growth inhibition in MCF-7 human breast cancer cells.
… of estrogen (E2) dependent human breast cancer cells. This growth inhibition was found to be associated with an accumulation of cells in G1 phase of the cell cycle. Since the molecular mechanisms underlying these antiproliferative effects were unknown, we investigated the effects of retinoic …
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Identification, Characterization and Mechanism of the Estrogen Regulation of the Gene Encoding the Sodium /Hydrogen Exchanger -Regulatory Factor (nhe-Rf)
… was used to visualize NHE-RF in MCF-7 cells. Secondly, we created adenovirus that express the wild type or anti-sense NHE-RF RNA. By infecting cells with the adenovirus we may overexpress or functionally ""knock out"" the NHE-RF protein and monitor the resulting effects on cell …
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Free radical formation and DNA damage in the cytotoxicity of alkylaminoanthraquinone antitumour agents.
… (AQ) based on MIT has been investigated in MCF-7 human breast cancer cells. lAQ, 1,8AQ and DOX were found to redox cycle in MCF-7 cells as evidenced by stimulation of NADPH oxidation, superoxide anion formation and hydroxyl radical formation associated with generation of a drug free radical …
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A novel intracellular protein delivery system - Magnesium phosphate nanoparticles with cationic lipid coating for catalase intracellular delivery
… vitro catalase release behavior was conducted on MCF-7 cells and EA.hy926 cells. LP MgP NP-CAT was delivered into MCF-7 cells and the release behavior was determined by the resultant resistance of the cells against hydrogen peroxide using MTS cell viability assay. The delivery of LP MgP NP-CAT …
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Chemical and biological evaluation of palythoa tuberculosa collected from the red sea
… activity on three human cancer cell lines (MCF-7, HeLa, and HT- 29). Palysterol G, in particular, was able to induce apoptosis in breast adenocarcinoma(MCF-7) cells
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Micrornas That Affect Breast Cancer Growth, Metastasis And Estrogen-Independent Growth
… Similarly, breast cancer cell lines including MCF-7 and MDA-MB-231 express a much lower level of mir-205 than the non-malignant MCF-10A cells. Of interest, ectopic expression of mir-205 significantly inhibits cell proliferation and anchorage-independent growth as well as cell invasion. …
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Pharmacological regulation of c-myc gene expression in human breast cancer cells
… cycle arrest and inhibition of proliferation in MCF-7 human breast cancer cell line (Woodfork, K. et al., 1995). The goal of studies reported here was to understand the molecular mechanisms of c-myc gene regulation by quinidine. C-myc is one of the most common oncogene aberrations in breast …
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Hormonal modulation of sex steroid hormone receptors and oncogenes in human breast cancer cells
… response closely followed the RNA response. In MCF-7 cells, which contain high levels of ER and an estradiol (E$\sb2$) induced PR, 1 nM E$\sb2$ caused a 60% drop in ER mRNA (6.6 kb), a 10-fold rise in PR mRNA (5 species: 11.4, 5.8, 5.3, 3.5, 2.8 kb), and a 60% drop in HER-2neu mRNA levels (4.8 …
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Impact of Vimentin Intermediate Filaments on 3D Multicellular Collective Behavior
… role in a collective cell cluster, single MCF-7 cells are embedded in a Matrigel-Alginate gel, which then grow into multicellular systems. The MCF-7 cells utilized are vimentin null, chemically inducible to form vimentin networks that interact with the other components of the cytoskeleton. …
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Circadian modulation of the estrogen receptor alpha transcription
… promoter decreases ERα mRNA expression levels in MCF-7 cells. Our work supports a circadian regulation of ERα through the repression of esr1 by Per2 in MCF-7 cells.
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Altered estrogen receptor and transforming growth factor pathways in models of estrogen-autonomous and of antiestrogen-resistant MCF-7 human breast cancer cells
… used clinically. We developed two models in the MCF-7 cell line, an estrogen receptor-positive human breast cancer cell line, to assess the relative importance of the estrogen receptor pathway and the transforming growth factor (TGF) pathways during this progression. MCF-7 cells maintained …
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Investigating senescence and senescence-escape in cancer cells
… role, the presence of senescent tumour cells and the ability of cancer cells to escape from senescence could potentially lead to therapy resistance and tumour recurrence. While a wide range of chemotherapeutic drugs are known to induce senescence in cancer, the effect of TIS in tumour …
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