Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 31 for “"MC3T3-E1"”.
-
Effect of Prostaglandin E2 on Mechanical Stresses Applied by MC3T3-E1 Osteoblast-like Cells on a Soft Hydrogel Substrate
… These observations also suggest that the MC3T3-E1 cells communicate with one another mechanically by changes in substrate surface pattern by redistribution of the stresses applied but not by changes in the magnitude of these stresses. The Levenberg-Marquardt algorithm was found to perform …
-
Proto-oncogene Regulation by Growth Factors in Bone Cells
… results from these model systems. In contrast, MC3T3-E1 cells are a clonal mouse osteoblast-like cell line. Since MC3T3-E1 cells consist of a single population of calvarial cells, they make an ideal system in which to study the autocrine effects of bone growth factors.</p> <p>Based on this work, …
-
Development of poly(3-octylthiophene) thin films for regulating osteoblast growth
… of poly(3-octylthiophene) (P3OT) to sustain MC3T3-E1 osteoblast attachment and growth. The central hypothesis was that specific P3OT film properties (i.e., thickness, film preparation conditions, and level of doping) are able to regulate osteoblast functions (i.e., attachment and …
-
In Vitro Assessment of Bone and Cartilage Stimulating Peptides
… Osteoprogenitor cells examined included MC3T3-E1 preosteoblasts, human bone marrow-derived mesenchymal stem cells (hBM-MSCs), human periosteum-derived stem cells (hPDSCs), and rabbit periosteum-derived stem cells (rPDSCs). Both hBM-MSCs and MC3T3-E1 preosteoblasts exhibited a significant …
-
Bioluminescence Imaging Strategies for Tissue Engineering Applications
… chapter, the system was tested using MC3T3-E1 cells transfected with a constitutive bioluminescent reporter. It was found that bioluminescence imaging was possible with this system. In the second experimental chapter, MC3T3-E1 cells transfected with BMP-2 linked bioluminescence …
-
Einfluss von niederenergetischem gepulsten Ultraschall auf das Proliferations- und Differenzierungsverhalten osteoblastärer Zellen in vitro
… sowie um die murine Osteoblasten-Linie MC3T3-E1 und um die murine Fibroblasten-Linie L929. Die Ultraschallbehandlung dauerte 20 Minuten täglich und wurde an bis zu sechs aufeinanderfolgenden Tagen durchgeführt. Keine der drei untersuchten Zellarten zeigte eine Änderung des …
-
The Role of Annexin A6 in Osteoporosis
… death of osteoblastic cell lines, C3H10T1/2 and MC3T3-E1 induced by synthetic GC, dexamethasone (Dex). In vivo mouse results indicated that PT-H not only had an inhibitory effect on the bone loss caused by GC, but also promoted bone formation. We further clarified the molecular mechanisms behind …
-
조골세포 사멸 및 분화에서 GSK3b의 역할
… that dexamethasone (Dex) induced apoptosis of MC3T3-E1 osteoblasts through the GC receptor. Dex activated GSK3β, and inhibition of GSK3β by lithium, a pharmacological antagonist, or gene knock-down by siRNA prevented the Dex-induced apoptosis. Unexpectedly, Dex also activated p38 …
-
Osteoblast Response to Zirconia-Hybridized Pyrophosphate Stabilized Amorphous Calcium Phosphate
… To determine the osteoinductivity of Zr-ACP, a MC3T3-E1 mouse calvarial-derived osteoprogenitor cell line was used to measure cell response to Zr-ACP. To accomplish this objective, Zr-ACP was added to cell culture at different stages in cell maturation (days 0, 4 and 11). DNA synthesis, alkaline …
-
In vitro Differenzierung von Monozyten der Zelllinine RAW 264.7 zu Osteoklasten, deren Charakterisierung und Wechselwirkung mit Osteoblasten
… 11. Freies RANKL lässt sich in Überständen von MC3T3-E1-Osteoblasten nicht nachweisen. 5. Das von Osteoblasten in das Medium abgegebene OPG ist in der Lage, die durch RANKL induzierte Osteoklastogenese von RAW-Monozyten zu hemmen. 6. In Kokulturen von MC3T3-E1-Osteoblasten und RAW-Monozyten kann …
-
Novel Insights into CB1 Receptor Signaling and the Anabolic Role of Cannabinoid Receptors in Bone
… important signaling molecules in osteoblasts. In MC3T3-E1 osteoblastic cells, the CB1 antagonist, AM251, has been reported to induce increases in Runx2 mRNA, mineralized bone nodule formation, and activation of signaling molecules such as ERK and AKT (Wu et al., 2011). Studies from our lab …
-
The Effect of Lycii Radicis Cortex Extract on Bone Formation in vitro and in vivo
… of two osteoblastic cell lines: C3H10T1/2 and MC3T3-E1. I selected a Lycii Radicis Cortex (LRC), Lycium Chinense root bark, as the primary candidate. Treatment with LRC extract showed enhanced alkaline phosphatase activity and an increased expression of bone metabolic markers Alpl, Runx2, and …
-
Freeze casting of bioactive glass and ceramic scaffolds for bone tissue engineering
… function of murine osteoblastic cells (MLO-A5 or MC3T3-E1). Subcutaneous implantation in the dorsum of rats showed abundant tissue ingrowth into the pores of the columnar scaffolds and integration of the scaffolds with surrounding tissue. The results indicate that bioactive 13-93 glass scaffolds …
-
Creation of Ovalbumin Based Scaffolds for Bone Tissue Regeneration
… the body. It was shown through cell studies that MC3T3-E1 pre-osteoblast cells significantly increased in number on the 10% and 15% scaffolds, therefore cell proliferation occurred. Because of a positive cellular response, 10% GA scaffolds were used for differentiation studies that showed an …
-
SENSITIZATION OF HUMAN OSTEOSARCOMA CELL LINE 143B WITH CALCITRIOL FOR CISPLATIN THERAPY
… 143B-MM, 143-P and control murine osteoblast MC3T3-E1. Cell proliferation and sensitization effects were evaluated by MTS assay. Boyden chamber assay was used for migration/invasion studies. MMP activity was measured using Sensolyte kit and real time PCR for the detecting the expression of …
-
Controlled release of gentamicin from polyelectrolyte multilayers to treat implant-related infection
… products were generally nontoxic toward MC3T3-E1 osteoprogenitor cells. A reproducible in vivo rabbit bone infection model was developed to test the PEM coatings against sterile, uncoated placebos; subsequent in vivo experimentation demonstrated the proof-of-principle that an …
-
The Response of Preosteoblasts to Combined Shear and Thermal Stress for Bone Tissue Engineering
… enhanced osteogenic response. To achieve this, MC3T3-E1 cells were exposed to one of the following protocols for an hour: no stress (control), shear stress at 1 dyne/cm2 using a parallel plate flow chamber, thermal stress in a 42°C incubator, or combined shear and thermal stress (1 dyne/cm2 at …
-
DEVELOPMENT OF SYNTHETIC POLYMERS FOR BONE TISSUE ENGINEERING: ENGINEERING THE DEGRADATION RATE
… study with the murine cell line MC3T3-E1 demonstrated that there were no cytotoxic components that leached from crosslinked PBF networks. In addition, the PBF crosslinked networks were assessed for the ability to support mesenchymal stem cell (MSC) differentiation down the …
-
Ovalbumin-Based Scaffolds Reinforced with Cellulose Nanocrystals for Bone Tissue Engineering
… scaffolds. With a MTT Assay, it was shown that MC3T3-E1 preosteoblasts significantly increase in metabolic activity on 2 wt. % films and scaffolds, an indication of proliferation. All scaffolds had a net increase in metabolic activity suggesting overall biocompatibility for OVA scaffolds and …
-
Voltage Effects on Cells Cultured On Metallic Biomedical Implants
… effect of static voltages on the behavior of MC3T3-E1 pre-osteoblasts cultured on CoCrMo alloy (ASTM-1537) was studied to determine the range of cell viability and mode of cell death beyond the viable range. Cell viability and morphology, changes in actin cytoskeleton, adhesion complexes and …
Page 1 of 2