Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"MC38"”.
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Orthotopic liver metastasis mouse models of mismatch repair-proficient colorectal cancer recapitulate clinical inefficacy of immune checkpoint blockade
… metastatic CRC do not respond to ICB therapy. MC38 and CT26 are two of the most commonly used mouse syngeneic CRC cell lines in preclinical studies of colorectal cancer. In most of these preclinical studies, MC38 and CT26 are implanted under the skin in the hind flank of the mice where they …
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Zinc Finger Protein 36L1 (ZFP36L1): Gene Expression, Regulation and Interactions with Immune Receptors in Human Tumour Cells.
… cell lines such as THP1, Jurkat, B16, MCF7, and MC38. To study the function of ZFP36L1 a CRISPR-Cas9 system was used to generate ZFP36L1 knockout MC38 cell line. Our results show that ZFP36L1 limits production of TNF α and VEGF A. Our preliminary data also show co-localization of ZFP36L1 with …
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Proteomics based analysis of interactions between CEA cell adhesion molecule 1 (CEACAM1) and intracellular proteins in transfected tumor cells
… using the murine adenocarcinoma MC38 cell line transfected with human CEACAM1-L were performed to isolate cytoplasmic proteins binding to the unphosphorylated or tyrosine phosphorylated CEACAM1 long cytoplasmic domain. Mass spectrometric analysis of immunoprecipitates separated on …
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Functional Genomic Studies of Cancer Immune Evasion Using In Vitro and In Vivo CRISPR/Cas9 Genetic Screens
… coverage in vivo is feasible in models of MC38 (mouse colon cancer) and B16F10 (mouse melanoma) cell isografts. Furthermore, two separate genome-wide in vivo screens have been completed in MC38 cells, which have enabled the identification of a number of candidate genes. Initial results …
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Immunostimulatory activity and antitumor efficacy of mSOT201 immunocytokine
… A single dose of mSOT201 completely cured all MC38 and the majority of CT26...
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Identification and Knockout of Immunodominant Endogenous Retroviral Antigen in Murine Tumor Models
… commonly used for preclinical studies B16F10, MC38, 4T1, and CT26 were able to produce replication-competent eMLV able to infect other murine cells in vitro and T cells in vivo. Human tumors have not been shown to produce infectious ERV, and so we removed the infectious potential of these …
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Mechanistic Elucidation and Therapeutic Improvement of Anti-CTLA-4 Therapies
… achieved more long-term cures than b1s1e2-Fc in MC38 tumors, demonstrating that CTLA-4 antagonism provided additional survival benefit. Consistent with prior reports that CTLA-4 antagonism enhances priming, treatment with 9d9, but not b1s1e2-Fc, increased the percentage of activated T cells in …
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An Affinity Threshold for Maximum Efficacy in Anti-PD-1 Cancer Immunotherapy
… Through in vivo efficacy studies using the MC38 murine adenocarcinoma model, we found that there is an affinity threshold required for maximum efficacy at a given dose of anti-PD-1 immunotherapy. We demonstrate that efficacy can be rescued by increasing the dose of a low affinity clone. Thus …
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Role of the Gut Microbiome in Cancer Immunotherapy Efficacy and Side Effects
… and TAK-242 to inhibit TLR4 signalling in MC38 tumour-bearing mice, the study demonstrated that hexa-acylated LPS enhances anti-PD-1 efficacy via TLR4 signalling, whereas penta-acylated LPS has minimal effects. These findings suggest that gut microbial composition and LPS acylation patterns …
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Elucidating the functional states of tumor-resident dendritic cells that drive productive anti-tumor immunity
… anti-tumor immunity) with a progressing tumor (MC38-SIY; dysfunctional anti-tumor immunity). We identified a novel activation state of CD11b+ conventional DC expressing an interferon-stimulated gene signature (ISG+ DC) that was enriched in regressor tumors. Like DC1, ISG+ DC was capable of …
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Precision Cytokine Therapy: Engineering and Delivery of CD8- and CAR-Tropic Interleukin-2 Variants
… experiments of purified proteins against an MC38 subcutaneous tumour model in vivo demonstrated the superior anti-tumour properties of scFv-IL2 relative to wild type IL2. In addition, IL2 variants designed to selectively support chimeric antigen receptor (CAR) T cells were developed. The most …
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Assessment of immunological responses to tumour antigens relevant to the development of therapeutic cancer vaccines
… demonstrated prolonged survival of the MC38 (naturally express mutated p53) cell-induced tumour bearer C57BL/6 mice in therapeutic vaccination setting and was found to be associated with enhanced tumour-infiltrating CD8+ lymphocytes (TILs). Prostate cancer (PrCa) is one of the most …
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C/EBPβ Regulates Tumour Size and Microenvironment Composition in Lewis Lung Carcinoma
… Bulk RNA-seq of Lewis lung carcinoma (LLC) and MC38 colon carcinoma cells grown as adherent monolayers or hanging-drop spheroids revealed a conserved “core spheroid signature” of 1, 527 genes, characterized by enrichment of autophagy-related pathways and suppression of cell-cycle programs. …
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Developing Novel Approaches to Improve Response to T Cell Based Cancer Immunotherapy
… <em>in vivo</em> murine studies using the MC38/gp100 tumour model show that ganestespib in combination with immune checkpoint blockade, resulted in superior antitumour effect and survival compared to either treatment alone. Microarray analysis of human cell lines treated with ganetespib …
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Studying the B and plasma cell responses to the immune checkpoint blockade treatment of cancer
… but also in other tumour cell lines (YUMM1.7 and MC38). This complemented the AgRSR mouse data and further suggests that the TdLN humoral immune response is directed against conserved self-antigens. In summary, this thesis has performed the first characterisation of how the TdLN B and plasma cell …
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Vergleich von rekombinanten Vaccinia- und DNA-Vektoren zur Tumorimmuntherapie im C57BL/6-Mausmodell
In der vorliegenden Arbeit wurden Tumorimpfstoffe auf der Basis des Plasmid-Vektors pCI, modified vaccinia virus Ankara (MVA) und MVA-infizierten dendritischen Zellen entwickelt und durch Sequenzierung, Western blotting und durchflußzytometrische Analyse überprüft. Die in vivo Wirksamkeit der …