Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"MBD2"”.
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The Role of the Methyl DNA Binding Domain Protein 2 (MBD2) in Breast Cancer
… work that the Methyl Binding Domain Protein 2 (MBD2) is necessary for the persistent repression of critical tumor suppressor genes in breast cancer. We show that stable knockdown of MBD2 also leads to growth suppression in cultured human mammary epithelial cancer lines (MCF-7, 49% suppression; …
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Efficacy and Mechanism of Â-Defensin2 Fused Antigen Protein Vaccines
… strategy of fusing adjuvant murine â-defensin2 (mBD2) to an antigen in order to elicit stronger anti-antigen immune responses are referred to as murine â-defensin2 (mBD2) vaccines. Previous studies have validated the potential of mBD2 vaccines, thus in this study we focus on increasing vaccine …
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Defensins In Ocular Immunity
… expression was either constitutive (mBD1 and mBD2) or</p> <p>inducible (mBD3 and mBD4) in normal BALB/c and B6 corneas, and disparately</p> <p>regulated in BALB/c vs B6 corneas after infection. Knock down studies using siRNA</p> <p>treatment indicated that mBD2 and mBD3, but neither mBD1 nor …
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The Role of DNA Methylation and Methyl Binding Domain Protein 2 in the Regulation of Human Embryonic and Fetal Beta Type Globin Genes
… mice null for methyl binding domain protein 2 (MBD2) express a similar level of γ-globin mRNA. DNA methylation and MBD2 appear to induce γ-globin expression via the same pathway(s), as treatment of MBD2 null βYAC transgenic mice do not show an additive boost in γ-globin expression. MBD2 does not …
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Methyl-CpG-Binding domain proteins and histone deacetylases in the stage-specific differentiation of olfactory receptor neurons
… with repressor complexes recruited by both MBD2 and MeCP2. HDAC1 and HDAC2, however, are divergently expressed in the OE, a pattern that is recapitulated in the brain. Rather than simultaneous inclusion in a complex, therefore, the individual association of HDAC1 or HDAC2 may provide …
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The Role of the Nucleosome Remodeling and Histone Deacetylase (NuRD) Complex in Fetal γ-Globin Expression
… gene. Chromatin modifying complexes, including MBD2-NuRD and GATA-1/FOG-1/NuRD play a role in γ-globin gene silencing, and Mi2β (CHD4) is a critical component of NuRD complexes. In the studies presented in Chapter 2, we observed that the absence of MBD2 in a sickle cell mouse model leads to a …
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Purification and Characterization of a Methyl-DNA Binding Protein Complex from Primary Erythroid Cells
… complex were identified in the sample: MBD2, RbAp48, HDAC2 and MTA1. Another identified protein, MENT, is a factor expressed only in chicken hematopoietic cells. These five proteins, as well as the MeCPl component Mi2, were found to tightly coelute by Western blotting of gel-filtration …
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Signaling mechanisms that suppress the anabolic response of osteoblasts and osteocytes to fluid shear stress
… association with methyl-CpG-binding protein 2 (MBD2), and suppression of osteopontin expression in response to fluid shear stress. MBD2, known to be involved in DNA methylation and interpretation of DNA methylation patterns, may aid in fluid shear stress-induced suppression of anabolic bone …
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Regulation of mammalian SINE transcription
… and rodent SINEs are bound by MeCP2, MBD1 and MBD2. Both human and rodent SINEs were also shown to be occupied by HDAC1, HDAC2 and a component of SWI/SNF complex, Brahma. Human Alus were also found to be occupied by components of two corepressor complexes, SIN3 and NuRD. Whether MBD proteins …
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Methylated DNA detection using a high-affinity methyl-CpG binding protein and photopolymerization-based amplification
… I displayed three of the core members MBD1, MBD2, and MBD4 on the surface of Saccharomyces cerevisiae yeast cells. Using the yeast display platform, I determined the equilibrium dissociation constant of human MBD2 (hMBD2) to be 5.9+/-1.3 nM for binding to singly methylated DNA. I further used …