Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"MALDI-MSI"”.
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Imaging the distribution of agrochemicals in plants by MALDI-MSI.
… few agrochemicals can be studied in this way.MALDI is a highly adaptable soft ionisation technique that was established in the late 1980's through the developmental research of Tanaka and Hillenkamp. The versatility of MALDI-MS has been extended in recent years with the advent of protein …
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Investigation of protein induction in vascular-targeted strategies.
… utilise mass spectrometry imaging techniques (MALDI-MSI), in combination with conventional proteomic methodologies, to investigate protein induction in vascular-targeted strategies. Proteins thought to be involved in tumourigenesis and drug treatment resistance were observed along with the …
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Characterizing neural tissues with mass spectrometry imaging via enhanced computational approaches
… of interest; however, mass spectrometry imaging (MSI) detects analytes across a broad mass range without the need to target a specific analyte. When used with matrix-assisted laser desorption/ionization (MALDI), MSI detects analytes in the mass range of neuropeptides. MALDI MSI simultaneously …
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Investigation of Phosphatidylinositols and Phosphoinositides Using Matrix- Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
… desorption/ionization mass spectrometry imaging (MALDI-MSI) is a powerful analytical technique that captures the spatial distribution of biomolecules directly from tissue surfaces. Widely used in lipidomics, MALDI-MSI provides label-free, high-resolution imaging of lipid species in situ, making it …
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Detection and Imaging of Neurotransmitters in Rodent Brain Tissue Using FMP-10 and ZnO Nanoparticle Matrices with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is an analytical technique used to visualize the spatial distribution of molecules within biological samples. In our lab, we employ two complementary matrices, 2-fluoro-1-methyl pyridinium (FMP-10) and zinc oxide …
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Sample preparation methodologies for MALDI-MS imaging and related topics.
The diverse applications of MALDI MSI are explored in this thesis with an emphasis on the sample preparation procedure and method development for small molecule analysis for a range of samples. The two main themes that have been focussed on are the pharmaceutical and metabolomic applications of …
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Efficient Identification and Comprehension of Molecular Pathways Associated with Irradiation Induced Hepatic Carcinogenesis
… To address this gap in knowledge, RNA-Seq and MALDI-MSI were used to assess the effects of space irradiation on the pathogenesis of HCC. In particular, RNA-seq was used to determine transcriptional changes, and MALDI-MSI to determine lipid changes, in the hepatic microenvironment of ion …
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Proteomics characterization of HPV-independent Vulvar Cancer Tumour Progression and Metastasis by Mass Spectrometry
… cancer studies. Recent developments of cut-edge MALDI mass spectrometry imaging (MALDI-MSI) and nano- LC-ESI-MS label-free quantification (LFQ) provide new opportunities to reveal differential abundances and spatial distributions of proteins from vulvar cancer tissue samples. In this thesis, …
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HIV-Associated Neurocognitive Disorders: Role of Viral Reservoirs and Lipid Dysregulation
… human cultures of HIV-infected astrocytes using MALDI-MSI or MALDI-2 MSI. To further examine lipid dysregulation, we also developed a MALDI-2 MSI method to detect lipid classes and species that are not detected with regular MALDI-MSI, using the brain tissue sections of the AD 3xTg mouse model. We …
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Exploring chemical communication and chemical heterogeneity in biological systems with mass spectrometry imaging
… communities. Mass spectrometry imaging (MSI) is an untargeted and unlabeled analytical tool that enables us to look at molecular constituents in two or three dimensions. Exploring these additional spatial dimensions of heterogeneity is essential to understanding of specific molecular …
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Analysis of the composition of latent fingermarks by spectroscopic imaging techniques.
… of the work presented in this thesis utilises MALDI mass spectrometry in profiling and imaging mode to investigate latent fingermarks, with SERS and ATR-FTIR used as complementary techniques.One of the major issues of using analytical techniques to investigate latent fingermarks is their …
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The use of MALDI-MS for imaging drug disposition in respiratory disease models.
… desorption/ionisation-mass spectrometry imaging (MALDI-MSI) has been extensively applied to monitoring the distribution of pharmaceutical compounds in tissues. The main aim of the work reported in this thesis is to monitor the distribution of respiratory compounds in the lungs following inhaled …
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In defence of flowers: The ecology and biochemistry of a chemical defence, cyanogenesis, in Proteaceae
… in dissected floral tissues and used a new MALDI-MS imaging method to investigate and compare fine-scale patterns in the distribution of CNglycs within flowers of eight congeneric Lomatia species (Chapter 2), and 11 species from different genera from across the family phylogeny (Chapter 3). …
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Využití metod hmotnostní spektrometrie v identifikaci a lokalizaci významných molekul parazitických helmintů
Tato práce shrnuje základní technologická řešení, postupy a principy hmotnostní spektrometrie a zaměřuje se na její využití v oblasti parazitologie. V první části jsou postupně definovány jednotlivé fáze zpracování biologického vzorku s využitím hmotnostní spektrometrie - od ionizace až po způsoby …