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Showing 1 to 20 of 83 for “"MALDI-MS"”.
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Exploring single cell neurochemistry with multimodal MALDI MS
… genes within a cell, while mass spectrometry (MS) measures gene products and metabolites themselves. Single cells are inherently sample limited, so means of extending or expanding the amount of information that can be garnered from an individual cell is important for understanding their complex …
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Sample preparation methodologies for MALDI-MS imaging and related topics.
The diverse applications of MALDI MSI are explored in this thesis with an emphasis on the sample preparation procedure and method development for small molecule analysis for a range of samples. The two main themes that have been focussed on are the pharmaceutical and metabolomic applications of …
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DEVELOPMENT OF IONIC POLYMER NANOBRUSHES WITH APPLICATION IN MALDI-MS
… and their development will be evaluated in terms of the environmental stimuli response. Once an essential insight of the brush behavior is gained, application will be evaluated in terms of the protein fractionation prior to analysis by Matrix Assisted Laser Desorption Ionization Mass …
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Synthesis and Maldi-Ms Characterization of New Ruthenium Sulfide Clusters
… from the reaction of RuCl3(NO)(P(aryl)3)2 and S(TMS)2 were investigated. The distorted heterocubane Ru4 S4(NO)4(PPh3)3 was crystallographically characterized and a related cluster with formula Ru4S3(NO) 4(PPh3)3, possibly corresponding to a partial heterocubane structure, was identified using …
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Detection and imaging of pharmaceutical compounds in skin by MALDI-MS.
… compounds in porcine epidermal tissue by MALDI-TOF-MS is presented. Direct analysis of fresh tissue sections was compared with the analysis of tissue imprints formed by blotting onto a variety of substrates. The samples were coated with matrix material by a prototype electrospray …
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The use of MALDI-MS for imaging drug disposition in respiratory disease models.
… desorption/ionisation-mass spectrometry imaging (MALDI-MSI) has been extensively applied to monitoring the distribution of pharmaceutical compounds in tissues. The main aim of the work reported in this thesis is to monitor the distribution of respiratory compounds in the lungs following inhaled …
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Protein Biomarkers of Ionizing Radiation and Standardization by MALDI MS/MS and Quantification
… called a lateral flow device, to triage victims of a radiological dispersal device or nuclear detonation. Mice were radiated with 2 Gy, 4 Gy and 7 Gy and sacrificed at 1 hour, 24 hours and 72 hours. Also, mice were injected with lipopolysaccharide (LPS) endotoxin (10 ?g) to induce an …
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Off-Line CE/MALDI MS: Sampling and Deposition Techniques to Incorporate Complementary Analyses for Cellular Assays
The viability of this CE/MS method is demonstrated by the chemical profiling of tissues and single cells from the marine mollusc, Aplysia californica , assaying neuronal release, analyzing cone snail venoms, and incorporating radionuclide detection with CE/MALDI MS. The application of the CE/MS …
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Investigation of Primary Ion Formation Mechanisms in UV-MALDI-MS Using Excited State Dynamics of Common MALDI Matrices
… insight towards primary ionization mechanisms within MALDI mass spectrometry. Albeit MALDI-MS is an extensively used analytical technique, the mechanism in which primary ions are created is still under scrutiny. Two current models of primary ionization exist which claim to elucidate the ion …
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DEVELOPMENT OF A NOVEL MATRIX ASSISTED LASER DESORPTION / IONIZATION (MALDI) BASED PEPTIDE QUANTITATION APPROACH
Matrix Assisted Laser Desorption / Ionization (MALDI) Mass Spectrometry (MS) has emerged as an important tool in the field of proteomics mainly because it is simple, quick and efficient. The identification and quantitation of biomarkers, protein targets for drugs, and metabolites are some of the …
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Proteomic Profiling of Pro and Active Matrix Metalloproteinases using Tandem Mass Spectrometry. Optimization of Affinity Chromatography and nHPLC-MALDI-MS/MS for Proteomic discrimination of Matrix Metalloproteinases in pre-clinical Cancer Model.
… desorption ionization tandem mass spectrometry (MALDI-MS/MS) to help identify and discriminate the various MMP forms. Firstly, a triazine dye-based ligand immobilized on chromatography beads was utilized to assess whether it binds to recombinant human MMPs (rhMMPs). The results highlighted that …
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Novel Mass Spectrometric and Sampling Strategies for Investigating Neuropeptide Processing and Distribution
… sampling approaches such as microseparation with MALDI MS detection are necessary to pre-concentrate and partially purify neuropeptides and to reduce ionization suppression effects. Here we describe the use of capillary electrophoresis (CE) coupled with complementary detection schemes MALDI MS and …
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Development of Assays for Neuronal Peptide Content and Release
Neuropeptide release is also studied with MALDI TOF MS. In this method, a small chamber (10 $\mu$l) is used to hold an isolated bag cell cluster in a small volume of physiological saline. The chamber consists of a standard pipette tip with the narrow end melted around two sections of fused silica …
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RAMIE (BOEHMERIA NIVEA): AN UNDERUTILIZED PLANT FOR A MULTIPURPOSE INDUSTRIAL APPLICATION; A PERENNIAL PLANT WITH PROTEIN IDENTIFICATION USING MALDI-MS, A BIOSORBENT FOR Hg (II) REMOVAL FROM WATER AND AS A BAST FIBER PRODUCTION VIA ENZYMATIC DEGUMMING
<p>Identifying plant species that can offer life support to humans and animals without damaging the environment is quite challenging. Ramie, one of the oldest fiber crops was studied due to its nutritional value as fodder and other multipurpose applications in, e.g., medicinal, environmental, and …
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Fundamental aspects of imaging matrix assisted laser desorption ionisation mass spectrometry.
… the use of a high repetition rate laser for MALDI-MS image analysis by developing methodologies for the detection of a number of different compounds from a variety of biological tissues. Additional investigations include and examination of strategies for normalisation and statistical …
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Structural Study of Natural and Synthetic Polymers
… of zein, corn prolamines, was examined with MALDI-MS, TEM, SEC3, SAXS and viscometry. MALDI-MS is demonstrated to be a superior technique to study the molecular weight and composition of zein proteins than conventional SDS-PAGE. The observed large particle size from TEM and SEC3 compared to …
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MATRIX-ASSISTED LASER DESORPTION/IONIZATION (MALDI) TARGET MODIFICATION FOR ENHANCED PROTEOMICS ANALYSIS AND PLASMA POLYMER CHARACTERIZATION BY MALDI MASS SPECTROMETRY
… three surface modifications are used to produce MALDI targets having reduced surface-protein binding affinity with a goal of increasing peptide/protein MALDI ion signals and lowering the limits of detection (LODs) for proteins and peptides. The second section discusses a bioselective MALDI …
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Matrix-assisted laser desorption/ionization mass spectrometry for the analysis of collected bioaerosols
… detection and identification of microorganisms from bioaerosols using offline matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the analysis of proteins. MALDI-MS intact bacteria techniques were adapted for use with an orthogonal MALDI quadrupole-time-of-flight mass …
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Integrating Micro-Scale Separations to Matrix Assisted Laser Desorption and Ioniation Time of Flight Mass Spectrometry (MALDI-TOF-MS) for Protein Analysis
… and ionization time-of-flight mass spectrometry (MALDI TOF MS) for protein analysis. MALDI MS provides unsurpassed accurate mass measurements of intact bio-molecules, for example peptides and proteins, which in turn generate high molecular specificity enabling the identity, function and structure …
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Characterization, Functionalization and Applications of Alkyl Monolayers on Silicon Surfaces
… laser-activation modification of surfaces (LAMS), and multivariate analyses of time-of-flight secondary ion mass spectrometry (ToF-SIMS) images of LAMS spots were also reported. X-ray photoelectron spectroscopy (XPS) and other data show that alkyl monolayers prepared by scribing silicon under …
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