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Showing 1 to 20 of 32 for “"MALDI mass spectrometry"”.
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Multiscale MALDI mass spectrometry for tissue, cellular, and subcellular lipidomics
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-20 without embargo terms
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MALDI Mass Spectrometry Imaging for the Discovery of Prostate Carcinoma Biomarkers
… profiling of proteins in tissue sections using MALDI imaging mass spectrometry (MALDI-IMS) has the power to link molecular detail to morphological and pathological changes, enhancing the ability to identify candidates for new specific biomarkers. However, critical questions remain regarding the …
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TARGET MODIFICATION FOR ENHANCED PERFORMANCE MATRIX ASSISTED LASER DESORPTION IONIZATION (MALDI) MASS SPECTROMETRY
… MATRIX ASSISTED LASER DESORPTION IONIZATION (MALDI) MASS SPECTROMETRY MAJOR PROFESSOR: Dr. Gary R Kinsel MALDI MS, a powerful tool for the analysis of biomolecules, has undergone major advancement in instrumentation to yield improvements in robustness, sensitivity and throughput since its …
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Optimization of sample preparation in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry of macromolecules
… laser desorption ionization time-of-flight (MALDI-TOF) and Fourier transform mass spectrometry (MALDI-FTMS) analysis of low molecular weight polyvinyl acetate (PVAc) was developed and then applied to the characterization of commercially available chewing gum. The optimization of MALDI …
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Identification and Characterization of Bacterial and Bacteriophage Proteins Via Matrix-Assisted Laser Desorption Ionization (Maldi) Mass Spectrometry
… and characterization of the separated proteins (mass spectrometry). Protein analysis is an underutilized tool that can serve as a valuable alternative to DNA for the identification and characterization of microorganisms. Driven by the need to identify, characterize, and quantify proteins at ever …
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Oligosaccharide Analysis via Anion Attachment Using Negative Mode Electrospray (ES) and Matrix Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry
… cone voltage in negative ion mode electrospray mass spectrometry. Among them, fluoride and acetate have the abilities to significantly enhance the absolute abundance of [M-H]- for neutral oliogosaccharides. The chloride adduct has the best stability among all the adduct species investigated. For …
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MATRIX-ASSISTED LASER DESORPTION/IONIZATION (MALDI) TARGET MODIFICATION FOR ENHANCED PROTEOMICS ANALYSIS AND PLASMA POLYMER CHARACTERIZATION BY MALDI MASS SPECTROMETRY
… three surface modifications are used to produce MALDI targets having reduced surface-protein binding affinity with a goal of increasing peptide/protein MALDI ion signals and lowering the limits of detection (LODs) for proteins and peptides. The second section discusses a bioselective MALDI …
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Development of Amine Containing Polymer Modified MALDI Targets for Complex Peptide and Protein Mixture Fractionation Prior to MALDI Mass Spectrometry Analysis
… OF AMINE CONTAINING POLYMERIC MODIFIED MALDI TARGET SUBSTRATES FOR COMPLEX MIXTURE FRACTIONATION OF PEPTIDE AND PROTEIN MIXTURES PRIOR TO MALDI MASS SPECTROMETRY ANALYSIS MAJOR PROFESSOR: Dr. Gary Kinsel The focus of this dissertation is the synthesis, development and application of the …
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DEVELOPMENT AND OPTMIZATION OF POLYELECTROLYTE POLYMER BRUSHES FOR THE FRACTIONATION OF PEPTIDE AND PROTEIN MIXTURES PRIOR TO MALDI MASS SPECTROMETRY ANALYSIS
… of peptides and digested proteins prior to MALDI-MS analysis. Even though MALDI-MS analysis is increasingly used in proteomic applications, this method of analysis suffers loss of performance in the analysis of mixtures due to the ion suppression effect i.e. basic peptides are more easily …
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DEVELOPMENT AND OPTIMIZATION OF ON-PROBE AFFINTY CAPTURE (OPAC) MALDI MASS SPECTROMETRY FOR THE FRACTIONATION AND ANALYSIS OF COMPLEX PROTEIN MIXTURES
… surface that can be directly incorporated on the MALDI target. On-Probe Affinity Capture (OPAC) MALDI is a method that uses the RF pulsed plasma modified target surfaces for the protein purification, separation and identification all on the same single probe and one of the highest advantage of …
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Analysis of the composition of latent fingermarks by spectroscopic imaging techniques.
… of the work presented in this thesis utilises MALDI mass spectrometry in profiling and imaging mode to investigate latent fingermarks, with SERS and ATR-FTIR used as complementary techniques.One of the major issues of using analytical techniques to investigate latent fingermarks is their …
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Disulfide Cross -Linking in Protein Microspheres
… of techniques including SDS-PAGE, SEC, and MALDI mass spectrometry peptide mapping. Electrophoresis and chromatography confirm the presence of higher molecular weight protein units as the principle components of the microsphere shell. Treatment with a disulfide reductant established that …
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MICROFLUIDIC DYNAMIC ISOELECTRIC FOCUSING COUPLED TO MATRIX ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY
… Matrix assisted laser desorption/ionization (MALDI) mass spectrometry has evolved into a primary analytical tool for proteomics research. MALDI is fast and efficient and has a high tolerance to non-volatile buffers and impurities. The samples for MALDI are typically applied to solid supports …
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Detection and Imaging of Neurotransmitters in Rodent Brain Tissue Using FMP-10 and ZnO Nanoparticle Matrices with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is an analytical technique used to visualize the spatial distribution of molecules within biological samples. In our lab, we employ two complementary matrices, 2-fluoro-1-methyl pyridinium (FMP-10) and zinc oxide …
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Micrometer-scale chemical analysis using heated AFM cantilever and mass spectrometer
… atomic force microscope(AFM) cantilever and mass spectrometer. Simulations investigate the underlying transport mechanisms within the system and the impact of various design parameters. The way to optimize the system is suggested based on the simulation results. A heated microcantilever is …
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Preparation and characterization of functionalized surfaces for desorption-ionization mass spectrometry
Structural proteomics combined with mass spectrometry, is a rapidly evolving scientific discipline that focuses on the characterization of the three-dimensional structures of proteins, as well as their dynamics. However, Current standard practices require a large number of separate processing …
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Development of a novel electron-transfer secondary reaction matrix, characterization of the site–specificity of novel bilin-lyase, and Fundulus grandis protein expression investigation using mass spectrometry
… performed at the New Orleans Center for Mass Spectrometry at the University of New Orleans. The projects that are detailed in the coming pages take on a variety of subjects, but a common thread is that each employs matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to …
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Development of High-Throughput Platforms for Single-Cell Analysis
… on matrix assisted laser desorption/ionization (MALDI) mass spectrometry. We evaluated several materials and approaches to chip fabrication suitable for interfacing with a MALDI instrument. We also developed an analytical pipeline for efficient processing of cells on the chip and demonstrated its …
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Distinguishing Macrophage Activation States by Mass Spectrometry
… sections. The aim of this work was to find mass spectral biomarkers that allow the differentiation of activation states, and establish conditions that can be used in imaging mass spectrometry (IMS) experiments to investigate the spatial distribution of differently-activated macrophages …
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Post-translational modifications of SEL24K from salmon eggs and ZPA from Xenopus laevis eggs
… techniques employed for identification of PTMs, mass spectrometry has become a more and more important tool for detecting and mapping these covalent modifications and quantifying their changes. The two projects described in this dissertation focus mainly on the method development for …
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