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Showing 1 to 13 of 13 for “"MALDI imaging"”.
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Detektion relevanter Moleküle zur Diagnostik des Harnblasenkarzinoms mittels MALDI imaging
… practice. This work focuses on the technique of MALDI imaging. Compared to other proteomic tools MALDI imaging is a potential new technique providing higher sensitivity and specificity comparing and classifying fresh frozen tissue biopsies of bladder cancer. Using MALDI imaging different protein …
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Visualizing the Spatial Localization of Active Matrix Metalloproteinases (MMPs) using MALDI Imaging MS
… the ex vivo tissue sample was determined using MALDI imaging mass spectrometry. The MALDI imaging scheme employed a synthetic FRET substrate specific for MMP-2 and MMP-9. The intensity of the m/z value corresponding to the product of active MMP-2 and MMP-9 digestion in each spectrum was …
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Creating a Map for Surgeons: Direct Blotting Assisted Stamping of Tissue for MALDI Imaging Mass Spectrometry
MALDI imaging mass spectrometry is an attractive technique for the discovery of disease biomarkers from surgically removed tissue sections. It can provide spatial resolution and relative abundance of a wide range of biomolecules across the tissue surface. While the technology has advanced …
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Neue Methoden der Proteinanalytik zur Identifikation von diagnostisch relevanten Molekülen für das Harnblasen- und Prostatakarzinom
… to different proteomic techniques (2D-SDS-PAGE, MALDI- and SELDI-TOF-MS, MALDI imaging). In particular, analysis of magnetic bead based fractioned serum samples of both entities resulted in sensitivity and specificity values above the standard values when comparing both entities among themselves …
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Laser ablation sample transfer for mass spectrometry
… and matrix-assisted laser desorption/ionization (MALDI) analysis, the ablated material was captured in a static solvent droplet that was deposited on a MALDI target or flow-injected into a nanoelectrospray source. The direct analysis of biological fluids for off-line MALDI and electrospray was …
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Mass Spectrometry Imaging: Looking Fruits at Molecular Level
Mass spectrometry imaging (MSI) is a MS-based technique. It provides a way of ascertaining both spatial distribution and relative abundance of a large variety of analytes from various biological sample surfaces. MSI is able to generate distribution maps of multiple analytes simultaneously without …
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Sessile Droplet Microfluidic Platforms for Improvements in Mass Spectrometry Sample Preparation
… laser desorption/ionization mass spectrometry (MALDI-MS). Microscopy and MALDI imaging mass spectrometry (IMS) were used to assess analyte localization and matrix application methods. MALDI sample spot reduction using evaporative preconcentration was shown to increase detection sensitivities …
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MALDI Mass Spectrometry Imaging for the Discovery of Prostate Carcinoma Biomarkers
… profiling of proteins in tissue sections using MALDI imaging mass spectrometry (MALDI-IMS) has the power to link molecular detail to morphological and pathological changes, enhancing the ability to identify candidates for new specific biomarkers. However, critical questions remain regarding the …
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Biomarker identification in HIV and non-HIV related lymphomas
… biomarkers for DLBCL were determined using MALDI imaging mass spectrometry (IMS) and characterised using LCMS. The expression of one of the biomarkers, heat shock protein (Hsp) 70, was confirmed on a separate cohort of samples using immunohistochemistry. Our results indicate that the …
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Investigation of protein biomarkers in HIV positive and HIV negative associated DLBCL
… HIV and non-HIV related DLBCL were subjected to MALDI-imaging, in order to get the spatial distribution of proteins in DLBCL tissue. Proteins were extracted from tissue samples and subjected to liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) to identify proteins present in …
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Investigation of nutrient solutions for the hydroponic growth of plants
… compounds in plant tissue. Techniques including MALDI-MS, LA-ICP-MS and HPLC-ICP-MS have been utilized in the work presented here to demonstrate the distribution and accumulation of elements and compounds within plant material that has been grown hydroponically. The use of a zinc based seed …
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Využití metod hmotnostní spektrometrie v identifikaci a lokalizaci významných molekul parazitických helmintů
Tato práce shrnuje základní technologická řešení, postupy a principy hmotnostní spektrometrie a zaměřuje se na její využití v oblasti parazitologie. V první části jsou postupně definovány jednotlivé fáze zpracování biologického vzorku s využitím hmotnostní spektrometrie - od ionizace až po způsoby …