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Showing 1 to 20 of 22 for “"MALDI/TOF-TOF"”.

  1. The identification of biomarkers of chemotherapy resistance in breast cancer using comparative proteomics

    Hodgkinson, Victoria C.University of Hull2011

    … gel electrophoresis (2D-PAGE) with MALDI-TOF/TOF mass spectrometry and antibody microarray analysis. DEPs were submitted to Ingenuity Pathway Analysis (IPA) to identify any canonical pathway links, confirmed using western blotting and clinically validated in a pilot series of …

    hull Repository record

  2. Development of a novel electron-transfer secondary reaction matrix, characterization of the site–specificity of novel bilin-lyase, and Fundulus grandis protein expression investigation using mass spectrometry

    Boutaghou, Mohamed N

    … matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to solve a problem. Fundamental aspects of MALDI in-plume ionization are implicated in the introduction of a newly developed electron-transfer secondary ionization matrix. The remaining projects are related to the ever …

    uno Repository record

  3. Protein Biomarkers of Ionizing Radiation and Standardization by MALDI MS/MS and Quantification

    Remsen, Tiffany D.University of Missouri--Columbia2014

    … proteins extracted from the tongue samples and MALDI TOF-TOF analysis used to identify the proteins. Absorption measurements of the 2D gel was used to quantify the proteins. IR induced up-regulated or down-regulated proteins found only in the samples exposed to radiation were troponin I, …

    missouri Repository record

  4. Effects of Extracellular Polymeric Substance Composition on Bacteria Disinfection by Monochloramine

    Coburn, Kimberly MaryUniversity of Toledo2012

    … Time-of-Flight/Time-of-Flight Mass Spectrometry (MALDI-TOF/TOF-MS). Canonical correlation analysis (CCA) and partial least square (PLS) regression modeling were employed to explore the changes that EPS underwent during the monochloramine disinfection process. The analyses results suggested the …

    ohiolink Repository record

  5. Post-translational modifications of SEL24K from salmon eggs and ZPA from Xenopus laevis eggs

    Zhao, Liang2018

    … digestion strategy in combination with MALDI-TOF mass spectrometry analysis. The disulfide bond pattern was found to be symmetrical in the tandem repeat sequence of SEL24K. More importantly, an interesting phenomenon of gas-phase scrambling of disulfide bonds was observed during MALDI …

    u-pacific Repository record

  6. Identification and Characterization of a Relish-type NF-kB, DvRelish in Dermacentor variabilis, the American Dog Tick

    Verhoeve, Victoria IreneVeterinary Medical Sciences - Pathobiological Sciences2018

    … via Western blot, immunofluorescence assay and MALDI-TOF/TOF mass-spectrometry. Tick infection assays were performed using microinjection and capillary feeding technique methodologies to identify dvrelish expression in response to SFG Rickettsia infection. Microinjection of 107 R. rickettsii …

    lsu-thes Repository record

  7. Formas de CRISP-3, HSP-1 e HSP-2 como candidatas a marcadores de congelabilidade do sêmen de garanhões da raça Mangalarga Marchador

    Silveira, Camila OliveiraUniversidade Federal de Viçosa2017

    … foi por espectrometria de massas usando MALDI-TOF/TOF, com o software Mascot Daemon para a busca em banco de dados. A validação das proteínas identificadas entre os tratamentos foi realizada pelo programa SCAFFOLD (95%). Os dados foram analisados pela ANOVA com 5 % de probabilidade de …

    brazil-ufv Repository record

  8. Charakterisierung von Proteinen und Untersuchung des IGF-Systems im Eberseminalplasma

    Hochschulz, AnjaLudwig-Maximilians-Universität

    … einer Seminalplasmaprobe wurde IGFBP-5 mittels MALDI-MS identifiziert. Ein Western-Blot mit gegen IGFBP-5 generierten Antikörpern wäre ein effektives Verfahren zur Ermittlung der IGFBP-5-Aktivität im Eberseminalplasma. Die Nutzung der Proteomics-Technik zur Darstellung des Proteinmusters im …

    lmu-germany Repository record

  9. Proteomic identification of putative biomarkers of neo-adjuvant chemotherapy resistance in luminal (ER+) breast cancer

    Hussain, TasadooqHull York Medical School, the University of Hull and the University of York2013

    … (antibody microarray/AbMA and 2D-PAGE with MALDI-TOF/TOF MS) to investigate a pilot series of breast cancer samples our research group recently identified 14-3-3 theta/tau, tBID and BcL-XL as putative biomarkers of response to neoadjuvant chemotherapy (Hodgkinson et al J Prot 2012, …

    hull Repository record

  10. Perfil de expressão protéica de larvas de Hysterothylacium sp. (Nematoda) de peixe espada (Trichiurus lepturus) da costa do Rio de Janeiro, Brasil

    Mantovani, CynthiaUniversidade Federal de Viçosa2014

    … apresentaram melhores resultados em relação ao MALDI-TOF/TOF. No total foram identificadas 87 proteínas de diversas famílias, dentre as quais julgamos importante citar as metabólicas, estruturais, ribossomais, sinalizadoras, reguladoras, transportadoras e, também, proteínas imunogênicas, segundo …

    brazil-ufv Repository record

  11. The Eosinophil Granular Leukocyte; Source and Target of High Mobility Group Protein B1

    Straub, ChristofThe University of Texas Medical Branch at Galveston2017

    … profiling project. The protein was identified by MALDI-TOF/TOF and its presence was confirmed by Western blot analysis. Given recent reports of HMGB1’s proinflammatory capabilities and a lack of knowledge regarding its role in eosinophilic disorders, we hypothesized that eosinophil-derived HMGB1 …

    utmb Repository record

  12. A Mass Spectrometry-Based Method For Quantification Of Human Metallothionein Isoforms And Usefulness In Biomarker Detection Of Cancers And Metal Toxicity

    Mehus, Aaron Andrew

    … liquid chromatography and analyzed by MALDI-TOF/TOF mass spectrometry. Relative expression was measured by combining 14N-labeled control and 15N-labeled cadmium-treated cytosols before trypsin digestion and determining monoisotopic peak ratios of the targeted peptides. Absolute …

    nodak Repository record

  13. Proteomic Analysis of the Blood of <i>Alligator mississippiensis</i>

    Darville-Bowleg, Lancia Nadinia FallenChemistry2017

    … the peptides were isolated and fragmented using MALDI TOF/TOF MS for <i>de novo</i> sequencing. Lectins are a class of carbohydrate selective proteins that are part of the complement immune system. Chapter 6 presents results for a lectin isolated from alligators that have mannan and mannose …

    lsu-thes Repository record

  14. The genetic and biochemical analysis of Drosophila Wwox protein function.

    Colella, Alexander2008

    … identified by mass spectrometry (MS) using both MALDI-TOF/TOF-MS and LC-ESI-MS/MS. Label free quantitative MS analysis was also performed in order to determine the most abundant protein(s) in those spots found to contain multiple proteins. These proteomic studies identified changes in a wide …

    adelaide Repository record

  15. Proteins and instability elements involved in phosphoenolpyruvate carboxykinase mRNA turnover

    Hajarnis, Sachin Shrikant, author, Curthoys, Norman, advisor, Bamburg, James, committee member, Mykles, Donald, committee member, Laybourn, Paul, committee member, Wilusz, Jeffrey, committee memberColorado State University. Libraries2006

    … pull down assays, western blot technique and MALDI-TOF/TOF analyses. The identified proteins bind to AU- and CU-rich sequences and secondary structures found within the PEPCK 3'-UTR. A tetracycline regulated promoter system was utilized in kidney proximal tubule-like cells, to determine the …

    colostate Repository record

  16. Early markers of breast cancer in nipple aspirate fluid

    Yafei HuangThe University of Texas Medical Branch2007

    … strategies, 2D gel analysis coupled with MALDI-TOF/TOF, and 1D gel coupled with LC-MS/MS, were used to characterize protein profiles of type I and II NAF. Using these two strategies, a total of 99 proteins were identified: 13 unique to type I NAF, 57 unique to type II NAF, and 29 common to …

    utmb Repository record

  17. Reduced cuticular penetration as a contributor to insecticide resistance in the common bed bug, Cimex lectularius L.

    Koganemaru, ReinaVirginia Tech2015

    … the matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) high-resolution tandem mass spectrometry (MALDI-TOF/TOF). The total of 265 peptides were identified, among which 206 belonged to one of 50 confidently identified proteins. We identified the CPRL, CPF, …

    vt Repository record

  18. Mass Spectrometric Analysis of Tyrosine Metabolic Enzymes

    Vavricka, Christopher JohnVirginia Tech2009

    … is a primary post-translational modification. Q-TOF mass spectrometry was used to determine N-glycosylation patterns from Aedes aegypti DCE and MALDI-TOF/TOF was used to determine multiple glycosylation sites in DCT. N-glycosylation is critical for the folding and trafficking of secreted proteins …

    vt Repository record

  19. Effects of Functionality and Charge in the Design of Acrylic Polymers

    Brown, Rebecca HuyckVirginia Tech2009

    … properties using this technique. Analysis with MALDI-TOF/TOF spectroscopy revealed the dominance of a back-biting side reaction at elevated temperatures. Hydroxy-functional random and block copolymers of n-butyl acrylate (nBA) and 2-hydroxyethyl acrylate were synthesized using nitroxide mediated …

    vt Repository record

  20. Mass Spectrometry Studies on the Dissociation of Metal-Adducted Oligosaccharides, Glycopeptides, and Fatty Acids

    Akor, Chioma Jane-FrancesUniversity of Alabama Libraries2024

    … a matrix-assisted laser desorption ionization (MALDI)/tandem time-of-flight (TOF-TOF) mass spectrometer to obtain complete structural information on underivatized oligosaccharides, glycopeptides, and fatty acids. Extensive experimentation with various MALDI matrices and metal cations led to the …

    alabama Repository record

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