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Showing 1 to 20 of 35 for “"MAGs"”.
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Recovery and quality estimation of metagenomic assembled genomes of eukaryotes
… of so called metagenomic assembled genomes (MAGs), which provide contextualised access to genes of these elusive organisms. As most studies have focused on the recovery of prokaryotic MAGs, I first examined the limitations and gaps of existing tools with respect to their ability to recover …
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Expansion of Deltaproteobacteria diversity from marine sediment reveals unique metabolic features
… unique, uncultured metagenome-assembled genomes (MAGs) from a variety of coastal and deep-sea sediments. These 402 Deltaproteobacteria MAGs represent a 28% increase in Deltaproteobacteria genomes. Phylogenomic analyses revealed 12 novel lineages which consist entirely of uncultured …
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Prokaryotic diversity and ecosystem functioning in hypersaline environment: a metagenomic study of the Cagliari salterns
… salinities. Metagenome-assembled genomes (MAGs) of prokaryotic communities were reconstructed from both matrices in two seasons: winter and summer. The total number of MAGs passing quality thresholds was 2786, of which 1537 from SCV and 1249 from SM. Out of the total number of MAGs, 1131 …
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Conversion of triacylglycerols into monoacylglycerols by penicillium roquefortii
… have been devised to produce monoacylglycerols (MAGs) from butter and Shea oils with two strains of Penicillium roquefortii, FRR 2456 (isolated from a spoilt melon) and Wisbey PJ (a commercial dairy strain) A semi-micro method was developed using Preparative Thin Layer Chromatography (PTLC) and …
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Exploration of Respirable Dust Particles Sourced From Rock Strata in an Underground Coal Mine
… the presence of coal-mineral microagglomerates (MAGs) was demonstrated. The second line of study in Chapter 2 was therefore to explore whether MAG formation could be due to the RCMD generation process or environmental conditions—rather than merely an artifact of the sampling procedure—and the …
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Birth and After Birth and Painting Churches: Tina Howe's Examination of Love and Savagery in the American Family
… the abuse that lies at the heart of the play. Mags Church (short for Margaret) has come home to help her parents, Fanny and Gardner, pack their things; they are moving from Boston to their summer cottage in Concuit. A promising young artist on the rise, she is also going to paint a portrait of …
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Metagenomic approaches for examining the diversity of large DNA viruses in the biosphere
… I investigated 11 metagenome-assembled genomes (MAGs) of giant viruses previously surveyed from Station ALOHA in the Pacific Ocean. St. ALOHA is located near Hawaii and represents oligotrophic gyres which the majority of the ocean is made of them. I focused on 11 MAGs of giant viruses to get …
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Characterization of microbial primary and secondary metabolism in the marine realm
… depth. Analysis revealed a distribution of MAGs dominated by Chloroflexota and Thermoproteota, with diversity decreasing as temperature increased, consistent with a downcore reduction in subsurface biosphere diversity. Specific archaeal MAGs within the Thermoproteota and Hadarchaeota …
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Characterization of Microbial Primary and Secondary Metabolism in the Marine Realm
… depth. Analysisrevealed a distribution of MAGs dominated by Chloroflexota and Thermoproteota, with diversity decreasing as temperature increased, consistent with a downcore reduction in subsurface biosphere diversity. Specific archaeal MAGs within the Thermoproteota and Hadarchaeota …
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Characterization of the expression patterns of the retrogene-parental gene pairs in the African malaria vector Anopheles coluzzii
… Mopti strain. Testis and male accessory glands (MAGs) were also investigated by this method in An. coluzzii. Available expression data support previously observed testis enriched expression of retrogenes and provides evidence for the predominate expression of retrogenes occurring in postmeiotic …
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State-of-the-art Approaches for Sequencing, Assembling and Annotating Naphthenic Acid Degrading Bacterial Metagenomes
… annotation of novel metagenomes - producing 72 MAGs and other 5432 circular contigs - 226 of which were putative phages. I also include state-of-the-art protein structure prediction and structure homology search tools, which greatly enrich annotations of novel sequences that are below the …
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Metagenomic analysis of the biodiversity and seasonal variation in the meromictic Antarctic lake, Ace Lake
… analysed using metagenome-assembled genomes (MAGs) from Ace Lake, revealed phylotypes that highlighted their adaptation to the lake environment. Synechococcus phylotypes were linked to complex interaction with viruses, whereas some Chlorobium phylotypes were inferred to interact with …
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Charting environmental protein families through planetary metagenomes
… συναρμολογημένων γονιδιωμάτων (MAGs), ακαλλιέργητων ιικών γονιδιωμάτων (UViGs) και μεγάλων συνόλων πρωτεϊνικών αλληλουχιών που προέρχονται από δημόσια διαθέσιμους μεταγονιδιωματικούς και μεταμεταγραφωμικούς πόρους. Η μελέτη επικεντρώνεται στη μεγάλης κλίμακας ομαδοποίηση …
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Genomic Diversity of Magnetotactic Bacteria from Underexplored Extreme Aquatic Habitats
… bacterial metagenome-assembled genomes (MAGs) were recovered. These MAGs belong to diverse taxonomic lineages across 14 phylum-level classifications, including groups known to contain MTB as well as novel lineages previously not found to harbour MTB. Functional annotation reveals a …
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Genomic Diversity of Magnetotactic Bacteria from Underexplored Extreme Aquatic Habitats
… bacterial metagenome-assembled genomes (MAGs) were recovered. These MAGs belong to diverse taxonomic lineages across 14 phylum-level classifications, including groups known to contain MTB as well as novel lineages previously not found to harbour MTB. Functional annotation reveals a …
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Investigating a novel Asgard archaeon and its tubulin-like proteins
… eukaryotic cell. Metagenome-assembled-genomes (MAGs) from various marine environments have shown that this kingdom, officially called Promethearchaeati, encodes many Eukaryotic Signature Proteins (ESPs); proteins that were once thought to be unique but ubiquitous to eukaryotic cells. The …
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Omics-driven and constraint-based modelling of microbial community metabolism
… models (GEMs) via metagenome-assembled genomes (MAGs) and predict nutritional dependencies within communities directly from shotgun metagenomic samples. I applied this workflow to five datasets: synthetic lab cultures, human gut, plant associated, bulk soil, and ocean metagenomes, reconstructing …
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Análisis Metagenómico del Viroma Intestinal en Pacientes Intrahospitalarios y de Unidad de Cuidados Intensivos de Bogotá, Colombia
… en Genomas Ensamblados a partir de Metagenomas (MAGs) bacterianos, llamó la atención la presencia de dos familias virales (Myoviridae y Siphoviridae) en la misma especie bacteriana, Alistipes putredinis. Los resultados sugieren que las interacciones bacteria-bacteriófago pueden no ser siempre …
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Marine carbohydrate-active enzymes illuminate microbial ecology, evolution, and carbonate precipitation
… a widespread potential for EPS degradation among MAGs from Shark Bay pustular mats and suggest distinct roles for many phyla that are reported at high abundances in mats. Taken together, the literature reviews and new results presented in this thesis demonstrate that marine CAZymes are an exciting …
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Molecular characterization of microbial communities in small-scale biodigesters using whole genome shotgun metagenomic approach
… contigs, and metagenome-assembled genomes (MAGs) can achieve a more comprehensive understanding of microbial taxonomy and functional capabilities in biodigesters. This study investigates the microbial communities in small-scale biodigesters, using taxonomic data from various sources to …
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