Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"MAC-T cells"”.
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Effects of Growth Hormone and Insulin-Like Growth Factor-I on Milk Protein Gene Expression and Nutrient Uptake and Cell Proliferation in Clonal Bovine Mammary Epithelial Cells
… in bovine mammary epithelial cell line MAC-T cells were determined. GH increased (P < 0.01) expression of four major milk protein genes in MAC-T cells transfected with GHR expression plasmid. Cotransfection analyses indicated that GH also stimulated (P < 0.01) luciferase reporter gene …
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Effects of ovarian steroids on bovine mammary epithelial cells: in vitro and in viro evidence of indirect stimulation of proliferation
… hormone responsive proliferation of Mac-T cells, a recent clonal bovine mammary epithelial cell strain. Mac-T cells responded to all hormones tested as would be expected in vivo. Additionally, passage, harvesting, quantification, freezing, and co-culture techniques were modified to …
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Peptides can be utilized as amino acid sources for protein accretion and cell proliferation by cultured animal cells
… and cell proliferation in C₂C₁₂ myogenic, MAC-T mammary epithelial and ovine myogenic satellite cells. Factors in serum that may be involved in regulating peptide utilization was investigated using MAC-T cells. Growth of MAC-T cells was studied in the presence of methionine-containing …
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Regulatory factors of milk fat synthesis in dairy cows
… In the first study, bovine mammary epithelial (MAC-T) cells were treated with sterol regulatory element binding protein-1 (SREBP-1) specific siRNA. The mRNA and protein expression of SREBP-1 were decreased by more than 90% by siRNA. Fatty acid (FA) synthesis, uptake, and selected lipogenic …
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Peptides as amino acid sources for the synthesis of secreted proteins by mammary tissue explants and cultured mammary epithelial cells
… CD-1 mice and cultured bovine mammary epithelial cells (MAC-T) were used as experimental models. Explants and cultured cells were incubated at 37°C in a humidified atmosphere of 90% air/10% CO₂ and 95% air/5% CO₂, respectively, for 1 to 24 h in Dulbecco's modified Eagle's medium containing …
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Regulatory Roles of Essential Amino Acids, Energy, and Insulin in Mammary Cell Protein Synthesis
… on cellular signals for protein synthesis. MAC-T cells were treated with EAA, insulin, glucose, and acetate to observe their individual and interactive effects on phosphorylation of mTOR, rpS6, S6K1, 4EBP1, eEF2, eIF2α, Akt, and AMPK. These signaling effects on protein synthesis rates were …
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Regulation of Bovine Mammary Epithelial Cell Response by Autocrine IGF-I and by Collagen I
… factor-I (IGF-I) signaling in mammary epithelial cells may be modified or interrupted by modifications in the cellular environment may lead to 1) methods to increase the growth and proliferation of normal mammary epithelial cells for an increase in the amount of milk produced on a per animal basis …
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Cloning and characterization of ovine insulin, insulin-like growth factor-I and -II genes
… into a bovine mammary epithelial cell line (MAC-T). Stable transfectants were induced with a synthetic glucocorticoid to produce secretable IGF-I. Transcript expression of pMMTV-IGF-IW was confirmed by Northern blot analysis and IGF-I was quantified in the medium of growing cells with RIA. …
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Systemic and Intracellular Trafficking of Long-chain Fatty Acids in Lactating Dairy Cattle
… mammary and heart tissue, but is not present in MAC-T cells, a bovine mammary epithelial cell line. When overexpressed in MAC-T cells, FABP3 does not appear to be rate-limiting for fatty acid uptake in vitro and did not alter lipid metabolism. The function of FABP3 in the mammary gland remains …
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Autocrine mechanisms of action of insulin-like growth factor-I (IGF-I) and hormonal regulation of expression of IGF-finding proteins in mammary epithelial cells
… proteins (IGFBPs) genes in mammary epithelial cells. To test the hypothesis that IGF-I affects growth of bovine mammary epithelial cells through an autocrine and/or paracrine pathway, several cell lines were developed expressing an ovine exon-2 containing IGF-I cDNA under the control of the …
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A Proteomic Analysis of Differentiation in the Mammary Epithelium
… states of differentiation in mammary epithelial cells from two species: in vitro proliferating and differentiated MAC-T cells (a bovine immortal cell-line), and primary mammary epithelial cells isolated from pregnant and lactating mice. When comparing the lists of proteins that differed in …
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The Expression of T-Box (Tbx3) in the Bovine Mammary Gland
… and Tbx3 expression in bovine mammary epithelial cells (MEC), the cell responsible for milk production. To test our hypothesis, MAC-T cells (MEC line) were treated with GH at 100 (GH100) or 500 (GH500) ng/mL or IGF-I at 100 (IGF100) or 200 (IGF200) ng/mL for 24 and 48 hours. As determined by …
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Using CRISPR/Cas9 to modify the genome of cattle
… been associated with increased milk production. MAC-T cells and Angus fetal fibroblasts were transfected with a Cas9 plasmid, pSpCas9(BB)-2A-GFP, and a ssODN to insert the desired mutation. Using the CRISPR/Cas9 system we were able to create a double strand break resulting in indels and deletions …
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In Vitro Binding and Transport Regulation by Endothelial Cells: Preliminary Studies looking at FIX and IGF-I
Endothelial cells separate the bloodstream from the underlying tissue and play a crucial role in vascular homeostasis. They also form an important barrier for vascular drug delivery. This thesis contains preliminary studies targeted at understanding the mechanisms of binding and transport across …
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Growth Factor and Extracellular Matrix Regulation of Heifer Mammary Development
… E increased proliferation of mammary epithelial cells. Coinciding with increased epithelial cell proliferation, IGF-I protein increased and IGFBP-3 protein decreased within mammary tissues. Thus, proliferation was associated with an apparent net increase in the biological availability of IGF-I …
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Role of Staphylococcus aureus GapC and GapB in immunity and pathogenesis of bovine mastitis
… attach and internalize into mammary epithelial cells, enables bacteria to escape from the effect of immunity and antibiotics by being hidden in the intracellular niche and thereby causing chronic recurrent intramammary infection. S. aureus also has the ability to become …