Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 22 for “"MAC-T"”.
-
Effects of ovarian steroids on bovine mammary epithelial cells: in vitro and in viro evidence of indirect stimulation of proliferation
… hormone responsive proliferation of Mac-T cells, a recent clonal bovine mammary epithelial cell strain. Mac-T cells responded to all hormones tested as would be expected in vivo. Additionally, passage, harvesting, quantification, freezing, and co-culture techniques were modified to …
-
Effects of Growth Hormone and Insulin-Like Growth Factor-I on Milk Protein Gene Expression and Nutrient Uptake and Cell Proliferation in Clonal Bovine Mammary Epithelial Cells
… in bovine mammary epithelial cell line MAC-T cells were determined. GH increased (P < 0.01) expression of four major milk protein genes in MAC-T cells transfected with GHR expression plasmid. Cotransfection analyses indicated that GH also stimulated (P < 0.01) luciferase reporter gene …
-
Peptides can be utilized as amino acid sources for protein accretion and cell proliferation by cultured animal cells
… and cell proliferation in C₂C₁₂ myogenic, MAC-T mammary epithelial and ovine myogenic satellite cells. Factors in serum that may be involved in regulating peptide utilization was investigated using MAC-T cells. Growth of MAC-T cells was studied in the presence of methionine-containing …
-
Peptides as amino acid sources for the synthesis of secreted proteins by mammary tissue explants and cultured mammary epithelial cells
… and cultured bovine mammary epithelial cells (MAC-T) were used as experimental models. Explants and cultured cells were incubated at 37°C in a humidified atmosphere of 90% air/10% CO₂ and 95% air/5% CO₂, respectively, for 1 to 24 h in Dulbecco's modified Eagle's medium containing hormones, …
-
Regulatory factors of milk fat synthesis in dairy cows
… In the first study, bovine mammary epithelial (MAC-T) cells were treated with sterol regulatory element binding protein-1 (SREBP-1) specific siRNA. The mRNA and protein expression of SREBP-1 were decreased by more than 90% by siRNA. Fatty acid (FA) synthesis, uptake, and selected lipogenic …
-
Regulatory Roles of Essential Amino Acids, Energy, and Insulin in Mammary Cell Protein Synthesis
… on cellular signals for protein synthesis. MAC-T cells were treated with EAA, insulin, glucose, and acetate to observe their individual and interactive effects on phosphorylation of mTOR, rpS6, S6K1, 4EBP1, eEF2, eIF2α, Akt, and AMPK. These signaling effects on protein synthesis rates were …
-
Cloning and characterization of ovine insulin, insulin-like growth factor-I and -II genes
… into a bovine mammary epithelial cell line (MAC-T). Stable transfectants were induced with a synthetic glucocorticoid to produce secretable IGF-I. Transcript expression of pMMTV-IGF-IW was confirmed by Northern blot analysis and IGF-I was quantified in the medium of growing cells with RIA. …
-
Regulation of Bovine Mammary Epithelial Cell Response by Autocrine IGF-I and by Collagen I
… by autocrine IGF-I and by collagen I. The MAC-T cell line was chosen as the cell model utilized in these investigations because it is an immortalized bovine mammary epithelial cell line known to retain hormonal responsiveness to IGF-I. It was hypothesized that the production of IGF-I by …
-
Xenogeneic transplantation of immortalized bovine mammary epithelial cells
… PSG-5 cells (a clonal derivative of the MAC-T cell line engineered to express ovine IGF-I ) were transplanted into the cleared mammary fat pads of recipient mice. Following transplantation, spheroidal cell structures were observed in the cleared mammary fat pads of immunocompetent control …
-
Systemic and Intracellular Trafficking of Long-chain Fatty Acids in Lactating Dairy Cattle
… mammary and heart tissue, but is not present in MAC-T cells, a bovine mammary epithelial cell line. When overexpressed in MAC-T cells, FABP3 does not appear to be rate-limiting for fatty acid uptake in vitro and did not alter lipid metabolism. The function of FABP3 in the mammary gland remains …
-
Autocrine mechanisms of action of insulin-like growth factor-I (IGF-I) and hormonal regulation of expression of IGF-finding proteins in mammary epithelial cells
… were generated by cotransfection of clonal MAC-T cells with the IGF-I expression vectors and a plasmid conferring resistance to hygromycin-B (HYG-B), using a calcium phosphate precipitation procedure. Induction of the MMTV-LTR with the glucocorticoid dexamethasone (DEX) was required for …
-
A Proteomic Analysis of Differentiation in the Mammary Epithelium
… in vitro proliferating and differentiated MAC-T cells (a bovine immortal cell-line), and primary mammary epithelial cells isolated from pregnant and lactating mice. When comparing the lists of proteins that differed in abundance in the two experiments, we observed many similarities in …
-
The Expression of T-Box (Tbx3) in the Bovine Mammary Gland
… for milk production. To test our hypothesis, MAC-T cells (MEC line) were treated with GH at 100 (GH100) or 500 (GH500) ng/mL or IGF-I at 100 (IGF100) or 200 (IGF200) ng/mL for 24 and 48 hours. As determined by real-time RT-PCR, we did not observe a change in Tbx3 expression in cells treated …
-
Using CRISPR/Cas9 to modify the genome of cattle
… been associated with increased milk production. MAC-T cells and Angus fetal fibroblasts were transfected with a Cas9 plasmid, pSpCas9(BB)-2A-GFP, and a ssODN to insert the desired mutation. Using the CRISPR/Cas9 system we were able to create a double strand break resulting in indels and deletions …
-
In Vitro Binding and Transport Regulation by Endothelial Cells: Preliminary Studies looking at FIX and IGF-I
… factor-I (IGF-I) transport across both BAEC and MAC-T cells, a mammary epithelial cell line, cultured on tissue culture inserts. IGF-I is a circulatory growth factor implicated in the regulation of cell division and tissue proliferation. Competitive binding experiments between 125I-IGF-I and …
-
Growth Factor and Extracellular Matrix Regulation of Heifer Mammary Development
… ability to stimulate in vitro proliferation of MAC-T cells. In conclusion, the overall results support the hypothesis that locally derived IGF-I regulates prepubertal heifer mammary development. However, ERa expression and extracellular matrix proteins also appear to be important regulators of …
-
Constituintes químicos isolados de Salvinia auriculata Aubl. e atividade antibacteriana de extratos brutos sobre estirpes de Staphylococcus aureus causadoras de mastite bovina
… a diversas infecções. Salvinia auriculata é uma macrófita aquática encontrada em climas tropicais. Trabalhos anteriores realizados pelo nosso grupo de pesquisa avaliou a promissora atividade antibacteriana da planta contra as bactérias causadoras da mastite bovina. O objetivo deste trabalho foi o …
-
Denial-of-Sleep Vulnerabilities and Defenses in Wireless Sensor Network MAC Protocols
… sensor network medium access control (MAC) protocols that leave them susceptible to denial-of-sleep attack. It then classifies these attacks in terms of an attacker's knowledge of the MAC layer protocol and ability to bypass authentication and encryption protocols. Attacks from each …
-
Microphysiometry Studies of Rapid Binding of Insulin-Like Growth Factor I by Parental and Transfected Mammary Epithelial Cell Lines
… the (transfected) SV40-IGF-I and the (parental) MAC-T cell lines was achieved to continue comparison studies of autocrine and paracrine stimulation of bovine mammary epithelial cells by IGF-I. This work was supported by the Whitaker Foundation Biomedical Engineering Grant.
-
The Role of Exogenous Somatotropin, Ovariectomy and Extracellular Matrix in Bovine Mammary Gland Development
The highly regulated maturation of the mammary gland is poorly understood. Our studies were designed to further characterize the role of ovarian hormones, growth hormone (GH)/IGF axis proteins and extracellular matrix (ECM) in the growth and development of prepubertal mammary glands. Prepubertal …
Page 1 of 2