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Showing 1 to 20 of 54 for “"M. smegmatis"”.
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Prevalence of the gene lsr2 among the genus Mycobacterium and an investigation into changes in biofilm formation when inactivating lsr2 regulated genes in Mycobacterium smegmatis
Mycobacterium smegmatis is a non-pathogenic model organism commonly used to study the human pathogen Mycobacterium tuberculosis. M. smegmatis has a rough colony morphology and is capable of forming biofilms, however, when the DNA-regulating protein Lsr2 is inactivated the bacteria forms a smooth …
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The Biochemistry of VapBC Toxin-Antitoxins
… of 45 VapBC TAs. In contrast Mycobacterium smegmatis, a model organism for M. tuberculosis, contains only one vapBC operon, thereby making it an ideal system to uncover the possible role(s) that VapBC proteins play in mycobacteria. This thesis describes the functional characterisation of …
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Riboswitch regulation of methionine metabolism and vitamin B12 uptake in mycobacteria – implications for drug susceptibility and pathogenesis
… of cobamides in mycobacteria using M. smegmatis as a model. To this end, the genetic capacity for de novo production and uptake of cobamide in host-associated and environmental mycobacteria was assessed, followed by direct validation in M. smegmatis. A combination of genetics, gene …
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Analysis of the multiple chaperonins of Mycobacterium smegmatis
… cpn60 genes. The non-pathogenic Mycobacterium smegmatis has three cpn60 homologues, while the pathogenic Mycobacterium tuberculosis has two. This study is a functional characterisation of the chaperonins of M. smegmatis. Expression of cpn60.1, cpn60.2 and cpn10, but not cpn60.3, was found to be …
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Functional significance of unique sequences in Mycobaterium smegmatis Ku proteins
… features of Ku protein from Mycobacterium smegmatis that include a lysine-rich extension at the C-terminus and a zinc-binding motif in the DNA-binding bridge-region. The unique C-terminal tail of M. smegmatis Ku contains several lysine-rich low-complexity PAKKA repeats that are absent from …
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Visualising the Mycobacterial Mutasome
… Here, a panel of fluorescent strains of M. smegmatis was developed to investigate expression and subcellular localization of ImuA′ and ImuB in live mycobacteria exposed to genotoxic agents. Using fluorescence microscopy, it was observed that, during prolonged genotoxic stress, single M. …
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CRISPRI-based high-throughput functional genomic approaches for use in mycobacteria
… genomic approaches in the model mycobacterium M. smegmatis. The first approach combines large-scale pooled oligonucleotide synthesis and nextgeneration sequencing, and is termed CRISPRi-Seq. A pooled library of 11 367 mutants, targeting 2 385 M. smegmatis genes with M. tuberculosis homologues, was …
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Roles of the Methylcitrate and Methylmalonyl-COA Pathways in Mycobacterial Metabolism and Pathogenesis
… and in the related, nonpathogenic species, M. smegmatis, using genetic mutants of either or both of the metabolic routes. It is shown here that, as for M. tuberculosis, M. smegmatis preferentially uses the methylcitrate cycle for growth on propionate. In the absence of the methylcitrate cycle …
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Characterization of protein secretion in Mycobacterium leprae using phoA fusions in Escherichia coli and Mycobacterium smegmatis
… using surrogate host bacteria, E. coli and M. smegmatis. Bioinformatics identified 24 proteins with high probability for secretion in M. leprae. Fifteen of 24 ML genes showed more than 50% amino acid homology with their M. tuberculosis counterparts and were studied for gene expression and …
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Construction and Use of a Transposon for Identification of Essential Genes in Mycobacteria
… screen using the model organism Mycobacterium smegmatis, 13 mutants with ATc-dependent growth were identified. Identification of the insertion sites by cloning and sequencing indicated that there were nine genetic loci containing transposon insertions upstream of essential gene candidates in M. …
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Structure/function analyses indicate novel roles for mycobacterial DnaQ homologs in genome maintenance
… of DnaQ protein (M. tuberculosis Rv3711c; M. smegmatis MSMEG_6275) might be linked to the mycobacterial gyrases that are responsible for DNA negative supercoiling following replication. The phylogenetic analysis also revealed highly conserved nucleotide excision repair (NER) proteins among …
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Functional elements of the promoter, leader and intergenic spacer regions of ribosomal RNA operon(s) of mycobacteria
… Their function was tested in E.coli and M. smegmatis. The sequences of the leader and intergenic spacer regions from eight and six species respectively were established after amplification by means of peR. Both leader and spacer regions contain antitermination elements and RNaseIII …
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Identifying biosynthetic pathways for mycobacterial cell wall components using transposon mutagenesis
… was developed. Non-pathogenic Mycobacterium smegmatis and the fish pathogen Mycobacterium marinum were used for generating Tn-mutant libraries. From the colony morphology screen, two out of eight genes identified from M. smegmatis Tn-mutants and eleven out of twenty from M. marinum Tn-mutants …
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Molecular mechanisms of DNA repair in Mycobacterium tuberculosis
… SRAP deletion mutants were generated for both M. smegmatis (MSMEG_1891) and M. tuberculosis (Rv3226c). Despite the fact that SRAP was upregulated in both M. smegmatis and M. tuberculosis following genotoxic stress, no DNA damage phenotype was detected in any SRAP deletion mutant using a variety of …
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The Mycobacterium smegmatis "Proteome" : effects of growth phase on total protein synthesis and on the response to heat shock
… the effect of growth phase of Mycobacterium smegmatis on total protein synthesis and on the heat shock response was investigated. De novo protein synthesis was monitored by labelling with 35 [S]methionine and the protein expression profiles analysed using one- and/two-dimensional …
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Glyoxylate Metabolism in Mycobacterium smegmatis
… studies of MLS in the saprophyte Mycobacterium smegmatis, and show that MLS, unlike ICL, is dispensable for growth on acetate or fatty acids. We also describe the dglycerate pathway in M. smegmatis, which enables malate synthase-deficient bacteria to utilize acetate and fatty acids as sole …
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Rapid, Quantitative Assessment of Antimycobacterial Water Disinfection based on the Firefly Luciferase Reporter Gene
… of M. avium strains MD1 and A5, Mycobacterium smegmatis strain VT307 and Mycobacterium bovis BCG strain Pasteur carrying the firefly luciferase gene (pLUC10) were constructed. In pLUC10-carrying strains of M. avium strain A5 and M. smegmatis strain VT307, a direct correlation was shown between …
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Whole-genome transposon mutagenesis to elucidate the genetic requirements for vitamin B12 biosynthesis and assimilation in mycobacteria
… maps of de novo cobalamin biosynthesis in M. smegmatis, a non-pathogenic saprophyte. To this end, a combination of whole-genome transposon (Tn) mutagenesis and next generation sequencing (TnSeq) was applied in M. smegmatis ΔmetE, a gene-deletion mutant in which the cobalamin-independent …
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Isolation, identification, and in silico analysis of mycobacteriophage OKCentral2016.
… of tuberculosis and leprosy. Mycobacterium smegmatis (M. smegmatis) mc2155 has been used as the model organism for studying mycobacteria due to its increased growth rate and non-pathogenicity. M. smegmatis mc2155 is commonly used to isolate mycobacteriophages from soil. Comparative genomic …
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Identification and isolation of growth-phase specific proteins of mycobacteria
… heat shock proteins of Mycobacterium smegmatis LR222. A growth curve was constructed using the ATP assay. This method was shown by B. A. Ntolosi to be the most accurate indicator of when the organism entered the various phases of growth. It was possible to determine that M. smegmatis …
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