Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"M protein"”.
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Streptococcal M protein and human C4BP
Antigenic variation of surface proteins allows microorganisms to evade the immune system of the infected host. This phenomenon represents an apparent paradox, because the variable protein must retain an important function, while its antigenic properties vary extensively. The surface associated M …
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Mouse Protection Assay For Measurement of Antibody and M Protein Antigen of Streptococcus Equi
… stimulated by vaccination with S. equi. M protein preparations. This passive mouse protection assay was then used in an opsonization inhibition assay developed to measure the M protein antigen of S. equi. Various antigen preparations were tested using this assay to study the effectiveness …
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Streptococcus pyogenes - a manipulator of human defences
… of S. pyogenes is the cell surface attached M protein. It has previously been shown that M proteins of certain serotypes form complexes with fibrinogen and that these can elicit a pathological response, which may contribute to the massive vascular leakage present in streptococ¬cal toxic shock …
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ASSESSING AN M MUTANT VESICULAR STOMATITIS VIRUS FOR THE TREATMENT OF GLIOBLASTOMA MULTIFORME BY DETERMINING APOPTOTIC MECHANISMS AND ANTIVIRAL RESPONSE
… (GBM), an aggressive brain tumor. Matrix (M) protein mutants of VSV have shown greater selectivity for killing GBM cells versus normal brain cells, compared to VSV with wild-type M protein. The goal of experiments presented here was to determine mechanisms of M protein mutant (M51R) VSV …
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Investigation of the Vesicular Stomatitis Virus Matrix Protein: Uncoating and Assembly
… replication, and assembly. The matrix protein (M) of VSV is a central component of the viral replication cycle. While being the smallest of the viral proteins it is multifunctional and is involved in uncoating, cytopathic effects (CPE), and assembly of the virus. M protein interactions …
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Regulation of Surface Proteins Assembly on the Wall of Gram-Positive Bacteria
<p>Cell wall peptidoglycan-anchored surface proteins are essential virulence factors in many Gram-positive bacteria. Attachment of these proteins to the peptidoglycan is achieved through a transpeptidation reaction, whereby sortase cleaves a conserved Cterminal LPXTG motif and attaches the protein …
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Vesicular stomatitis virus induced apoptosis occurs by a mechanism involving the activation of pro-apoptotic Bcl-2 proteins Bak and Bid and the inactivation of anti-apoptotic Bcl-2 proteins Bcl-XL and Mcl-1
… I address the question of what role Bcl-2 family proteins play in apoptosis induced by vesicular stomatitis virus (VSV) with wild-type (wt) M protein (rWT virus). My results demonstrate that of the two major proapoptotic multidomain proteins Bak and Bax, Bak is more important for the induction of …
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Formation of membrane domains induced by the G and M proteins of vesicular stomatitis virus
… These domains are composed mainly of viral proteins and they are enriched in certain lipid species. The properties of the two envelope-associated proteins of VSV, the glycoprotein (G) and the matrix protein (M), were investigated in reconstituted lipid vesicles in order to understand the …
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Induction of myosin cross-reactive antibody and cytolytic T cell responses in mice with Streptococcus pyogenes
… antigens, namely the anti-phagocytic factor M protein and host proteins, specifically myosin. Experimental animal model systems developed to date have predominantly relied on the immunization of adult animals with M protein emulsed in adjuvant. In the laboratory we have undertaken studies with …
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A Study of the Membrane Binding Properties of the Matrix Protein from Human Respiratory Syncytial Virus
… thesis describes the structure of the matrix (M) protein from human respiratory syncytial virus (RSV), and its interactions with model membranes composed of a range of lipids, which have been characterized using a variety of techniques. The M protein was expressed in E. coli with a histidine tag …
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Protein FOG at the interface between G streptococci and human host defence lines
… human defence lines are investigated. A novel M protein from GGS, denoted FOG, was isolated, recombinantly expressed, and purified. FOG-positive strains survive in human whole blood wheras a FOG-negative strain did not. Addition of soluble FOG, but not protein G, leads to restoration of survival …
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Studies of the pathogenesis of IgA nephropathy and Henoch-Schönlein purpura, with special reference to Streptococcus pyogenes infections and complement
… (GAS). GAS express the surface bound M protein, which varies in sequence between strains and in certain serotypes includes an IgA-binding region (IgA-BR). The complement system, an important part of the innate immune system, is activated during IgAN and HSN reflected by the common …
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Cloning and expression of the M-gene from the human coronavirus NL-63 in different expression systems
… in bacterial KRX cells with expression of the M protein in the pellet of the lysed bacterial cells. No M protein was seen in the supernatant of the lysed cells. Sf9 insect cells were infected with the recombinant pFastBac clones and P1 and P2 viral stocks were obtained. Protein expression occurs …
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Exploring Nipah virus strain differences through use of recombinant and chimeric viruses as well as utilization of a novel 3D human lung model
… NiVs expressing the fusion (F), attachment glycoprotein (G), and matrix (M) proteins of the alternate variant alone or in combination was generated. These chimeric viruses were evaluated in in vitro systems representing many of the primary cellular targets of NiV infection. While there appears to …
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Determining the Innate and Adaptive Immune Responses to Vesicular Stomatitis Virus Vaccine Vectors
… that incorporates two enhancing strategies; an M protein mutation (M51R) that prevents the virus from suppressing host responses and the addition of a vaccine adjuvant, bacterial flagellin, expressed intracellularly from the viral genome (M51R-F). The ability of the vectors to induce innate immune …
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Improving the Monitoring of Multiple Myeloma Patients Using Blood Based Markers of Disease
… of a monoclonal immunoglobulin termed the M-protein. Multiple myeloma is a complex disease, and despite great advances in both detection and treatment of the disease, there remains a vast amount of interest in improving patient outcomes. In the era of novel agents and clinical trials, …
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On the induction of inflammatory reactions by Streptococcus pyogenes
… thesis explores how different streptococcal proteins interfere with innate immunity, adaptive immunity, and blood coagulation. M protein is a surface-bound streptococcal virulence factor with antiphagocytic properties. M proteins can be released from the bacterial surface, and in the present …
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Investigation of the interplay of yellow fever virus structural protein epitopes and genetic diversity
… the contribution of the envelope (E) protein in YFV attenuation through its role in genetic diversity, tissue tropism and recognition by WT and vaccine specific antibodies that bind E protein. Using infectious clones (i.c.) of both Asibi and 17D viruses, structural chimeras with …
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Postmortem changes in meat quality and myofibrillar protein degradation in turkey breast muscle
… was assessed using measurements of colour and protein extractability, while quality characteristics of cooked product were assessed using cook yield and torsion gelometry. The extractability and degradation of 10 specific proteins was monitored using SDS-PAGE and Western blotting. Additionally, …
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RESISTANCE TO VESICULAR STOMATITIS VIRUS IS INTRINSIC TO PC3 PROSTATE CANCER CELLS AND CAUSES INHIBITION OF EARLY STEPS OF THE VIRUS REPLICATION CYCLE
… constitutively overexpress antiviral mRNAs and proteins. Combined with the delays at several steps of the viral life cycle, these observations led to the two hypotheses tested in Chapter 2, which address whether PC3 cell resistance is intrinsic to these cells or whether it is induced as a result …
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