Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"Lytic enzymes"”.
-
The efficiency of lytic enzymes for Ascaris eggs inactivation
Submitted in fulfilment for the Degree of Master of Applied Sciences in Biotechnology at Durban University of Technology, Durban, South Africa, 2024.
-
The Roles of the Germination-Specific Lytic Enzymes CwlJ1, CwlJ2, and SleB in Bacillus anthracis Spores
… layer, is carried out by germination-specific lytic enzymes (GSLEs). While the GSLEs of several other Bacillus species have been investigated, they have not been characterized in the pathogen B. anthracis. In this work three GSLEs, CwlJ1, CwlJ2, and SleB are identified in B. anthracis and are …
-
Analysis of Peptidoglycan Structural Changes and Cortex Lytic Enzymes during Germination of<i> Bacillus anthracis</i> Spores
Sporulation is a process of differentiation that allows capable cells to go into a dormant and resistant stage of life. To become active again, the spores must germinate into vegetative cells. One key process in spore germination is hydrolysis of the cortex peptidoglycan. This process has been …
-
Characterization of the Bacillus anthracis SleL Protein and its Role in Spore Germination
… must be depolymerized by germination-specific lytic enzymes (GSLEs) before the bacterium can become a metabolically active vegetative cell. By exploiting cortex lytic enzymes it may be possible to control germination. This could be beneficial in elucidating ways to enhance current …
-
Chloroplasts as bioreactors : high-yield production of active bacteriolytic protein antibiotics
… In this work, it was shown that three phage lytic enzymes (Pal, Cpl-1 and PlyGBS) could be successfully expressed at very high levels and with high stability in tobacco chloroplasts. PlyGBS expression reached an amount of foreign protein accumulation (> 70% TSP) that has never been obtained …
-
Expanding the Horizons of Enzybiotic Identification
<p>Recently, phage lytic enzymes (also known as endolysins or, simply, lysins) have received considerable attention as potential antibacterial agents. During the infective cycle of double-stranded DNA phage, these peptidoglycan hydrolases are responsible for digesting the cell wall of the host …
-
Investigating the enzyme activity of a <i>Clostridioides difficile</i> amidase complex
… While the endospore cortex is forming, the enzymes CwlD and PdaA convert N-acetylmuramic acid (NAM) into muramic--lactam (MAL). MAL serves as a recognition element for germination-specific lytic enzymes that degrade cortex peptidoglycan layers during germination. Without the MAL residues the …
-
Molecular analyses of YhcN/YlaJ and spore-associated proteins involved in germination of Bacillus cereus spores
… concerns the function of hybrid cortex lytic enzymes. Mother-cell expressed SleB and forespore-expressed SleB-CwlJ hybrid proteins are capable of efficient cortex hydrolysis during germination and, additionally, can be activated by exogenous CaDPA. Activation of SleB by CaDPA has …
-
Characterization of the chitinolytic system during the mycoparasitic interaction between Trichoderma aggressivum f. aggressivum and different host strains of Agaricus bisporus /
… antagonism of other fungi, is the secretion of lytic enzymes such as chitinases, which actively degrade a host's cell wall. Therefore, the intent of this study was to examine the production of chitinase enzymes during the host-parasite interaction of Agaricus bisporus (commercial mushroom) and …
-
Regulation of the Spore Cortex Lytic Enzyme SleB in Bacillus anthracis
… the cortex peptidoglycan by germination-specific lytic enzymes (GSLEs), resulting in cells that are easily killed. This work investigated the regulation of the GSLE SleB by other proteins in the spore. A full understanding of how GSLEs are held inactive in the dormant spore and are activated …
-
A study of the mechanisms of antagonism by the biocontrol fungi <i>Trichoderma </i>against wood decay basidiomycetes
… volatile antibiotics, laminarinase and chitinase lytic enzymes) against <i>Neolentinus lepideus</i> and <i>Trametes versicolor</i> fungi indicated that these were also dependent on media type. Study of production of iron chelating siderophores by <i>Trichoderma </i>species indicated that they may …
-
Structural and Functional Studies of Sensor Kinase RetS from Pseudomonas aeruginosa and Peptidoglycan Hydrolase SleB from Bacillus anthracis
… interaction pattern.<br /><br />PartII: The catalytic domain of the germination-specific lytic transglycosylase SleB from Bacillus anthracis displays a unique active site topology-<br />germination-specific lytic enzymes (GSLEs) that degrade the unique cortex peptidoglycan to permit resumption of …
-
Natural Products From Functional Screening of Soil Metagenomic Libraries
… of a diverse set of antibacterially active enzymes. Clones displaying zones of inhibition of growth of an assay strain in top agar bacterial overlays were predicted through bioinformatic analysis to encode peptidases, lipases and cell-wall lytic enzymes conferring antibiotic activity, and …
-
Development of methods for the microbiome analysis of formalin fixed paraffin embedded tissue specimens
… strategy, and the use of a mix of bacterial-lytic enzymes was found to provide a uniform cross-taxa bacterial lysis. The collection of methods developed were tested by 16S rRNA gene sequence analysis of protoblocks, murine FFPE faeces and human breast tumour samples. The collection of methods …