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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 302 for “"Lysis"”.
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Algae Lysis with Pulsed Electric Fields
… <p>This project examines a novel method of cell lysis through pulsed electric field (PEF) application that enables cost-effective extraction methods relative to current enzyme and sonication techniques.</p> <p>A theoretical model for cell membrane potential in the presence of electric field was …
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Single Fibrin Fiber Mechanical Properties and Lysis
Fibrinogen, one of the most abundant proteins in blood plasma, plays a central role in hemostasis and thrombotic disease. In the final step of the coagulation cascade, thrombin proteolytically converts fibrinogen to fibrin, which then forms a mesh of fibrin fibers. This mesh is the major structural …
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Plato’s Lysis and Aristotle’s Notion of Friendship
Plato’s Lysis forms the background for Aristotle’s analysis of friendship (philia, φιλία). The dialogue raises four central questions: who is the friend—the lover or the beloved? Are friends alike or unlike? Are friends good or bad people? And what is the deep ground of loving friends? Although …
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Investigation of CNT-induced Escherichia coli Lysis and Protein Release
… the medium. Consequently, CNTs can be used as lysis agents when it is undesirable to add an additional enzyme (lysozyme) to cause the release of intracellular proteins.</p>
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Agglutination and Lysis Tests With Erythrocytes Adsorbed With a Fraction of Vibrio Fetus
Made available in DSpace on 2014-12-05T22:03:59Z (GMT). No. of bitstreams: 1 5904558.pdf: 3169836 bytes, checksum: 2bf546ac7c68272f2051618247803381 (MD5) Previous issue date: 1959
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Nucleic acid sample preparation using magnetic ionic liquids as cell lysis and DNA extraction solvents
<p>Sample preparation is vital in nucleic acid analysis. Cell lysis is generally the first step in DNA sample preparation where the cell membrane is disrupted to release intracellular components such as DNA. Moreover, the isolation and enrichment of nucleic acids is essential to prevent false …
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Effect of homozygous lpr and gld mutations on the immune functions and induction of autoimmunity
… <i>lpr</i> DN T cells could mediate spontaneous lysis of certain tumor cells as well as mediate redirected lysis of various tumor targets when stimulated through the CD3/αβTCR complex and certain adhesion molecules, such as, CD44 and gp90<sup>MEL-14</sup>. The DN T cells constitutively …
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Investigating the functional roles of the viral frameshift protein 2B* in EMCV infection
… is considered to be a result of the rapid cell lysis caused by EMCV. This highly efficient method of viral release also induces a severe inflammatory response, leading to disease and death. Viral propagation is further heightened by the ability of EMCV proteins to antagonise the host innate …
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Alternate pathways of cytotoxic T lymphocyte and natural killer cell activation
… lytic activity of CTls and redirect CTl-mediated lysis to antigen-negative Fc receptor-positive target cells. Similar redirected lysis was also inducible using mAb MEL - 14, directed against the lymphocyte homing receptor for endothelium (gp - 90MEL-14). The redirected lysis induced by mAbs 9F3 …
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Nonspecific Cell-Mediated Cytotoxicity in the Cat
… require a 16-hour assay to express target cell lysis and preferentially lyse virus-infected targets. Several allogeneic, virus-infected cell lines were lysed by NC cells as were both uninfected and virus-infected xenogeneic targets. Uninfected feline cell lines were not susceptible to NC lysis. …
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Staphylococcus aureus Panton-Valentine leukocidin (PVL) alters human neutrophil viability and function
… plasma membrane of PMNs leads ultimately to cytolysis. However, this hypothesis has not been tested and previous studies indicate that levels of PVL <i>in vivo</i> are likely insufficient to cause host cell lysis. On the other hand, sublytic concentrations of PVL prime neutrophils for enhanced …
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Staphylococcus aureus Panton-Valentine leukocidin (PVL) alters human neutrophil viability and function
… plasma membrane of PMNs leads ultimately to cytolysis. However, this hypothesis has not been tested and previous studies indicate that levels of PVL <i>in vivo</i> are likely insufficient to cause host cell lysis. On the other hand, sublytic concentrations of PVL prime neutrophils for enhanced …
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Influence of osmotic forces and transbilayer membrane area imbalances on the stability and morphology of model membrane vesicles
… studies show that osmotically induced vesicle lysis is a very rapid event with most of the solute release occurring within the first 30 seconds. However. lysis results in only partial release of solute such that a residual osmotic gradient results. This residual gradient is similar to the …
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Understanding Primate Immunity to African Trypanosomes Through the Characterization of Trypanosome Lytic Factor Formation and Function
… the temporal mechanism of TLF-mediated lysis. Purification and characterization of TLF from human serum, murine models, and hepatocytes in culture were used to determine that TLF is generated in the liver, where APOL1, and under some conditions HPR, are assembled in/on the hepatocyte …
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Recombinant collagen production optimization in Escherichia coli
… to lytic efficiency (both the time required for lysis and the degree of lysis). The resulting process will enable lysis to take place in the bioreactor, thus avoiding the extra time and monetary cost of a separate cell homogenization step for E. coli disruption and endogenous protein release..
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A Quantitative Investigation of Selected Reactions in the Fibrinolytic Cascade
… work has shown that thrombin activatable fibrinolysis inhibitor (TAFI) was unable to prolong lysis of purified clots in the presence of Lys-plasminogen (Lys-Pg), indicating a possible mechanism for fibrinolysis to circumvent prolongation mediated by activated TAFI (TAFIa). Therefore, the effects …
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Tumor Cell Glutathione Metabolism: Therapeutic Implications
… cycle. The susceptibility of tumor cells to lysis by a flux of H<sub>2</sub>O<sub>2</sub>, such as generated by granulocytes or activated macrophages, or by the enzyme glucose oxidase, was augmented by interference with the GSH redox cycle. Depletion of tumor cell GSH was by 2 methods: …
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Taking a closer look: exploring the functional roles of P10 in baculovirus-infected cells
… in polyhedron formation, nuclear stability and lysis. The work presented here aimed to elucidate the functional role of this protein during virus infection of cell cultures. To investigate the role of P10 during infection, a variety of Autographa californica nucleopolyhedrovirus (AcMNPV) mutants …
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Transistor-based biosensing: expanding the functionality of field effect transistors
… design of heating/cooling, ultra-localized cell lysis, and electrical detection of nucleic acid amplification using an on-chip electrode, this thesis aims to enable the next generation of portable biosensors for primary care and on-site diagnostics. Chapter 2 presents an overview of the current …
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