Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Lysine acetylation"”.
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Regulation of The Expression and Lysine Acetylation of Pro-Inflammatory Molecules By Lipid-Modifying Enzyme (LPCAT2) in RAW264.7 Cells
… was studied. Analysis of the regulation of lysine acetylation by LPCAT2 helps to understand the role of LPCAT2 in protein acylation. The results show that LPCAT2 regulates the expression of CD14 and RNF19B. It also influences the expression of lysine-acetylated proteins. Finally, LPCAT2 and …
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Lysine acetyltransferase Gcn5-B regulates the expression of crucial genes in Toxoplasma and its function is regulated through lysine acetylation
Histone acetylation has been linked to developmental changes in gene expression and is a validated drug target of apicomplexan parasites, but little is known about the roles of individual histone modifying enzymes and how they are recruited to target genes. The protozoan parasite Toxoplasma gondii …
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Crosstalk of protein phosphorylation and lysine acetylation on 14-3-3 proteins and their applications in brassinosteroid signaling and photosynthesis
Protein phosphorylation and lysine acetylation are two important post-translational modifications (PTMs) in eukaryotes. However, the crosstalk between these two modifications is largely unknown. We demonstrated that both phosphorylation and lysine acetylation occurred to an Arabidopsis protein, …
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Quantitative approaches to probe the acetylproteome
Lysine acetylation is a prevalent post-translational modification whose multi-varied biological roles have recently emerged. While having all the necessary components of a signaling network, lysine acetylation studies have been limited to a small subset of proteins and pathways. Using a …
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Development and Application of Proteomic Technologies for the Analysis of Post-Translational Modifications
… bearing known modifications, including O-GlcNAc, lysine acetylation and methyl esterification. The identification of known targets of the modifications support some of the current ideas regarding their potential impact and serve as a means of validating the methods. More importantly, the …
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Investigation of parameters of chromatin higher order structure and the making of multiply-acetylated histone H3 via nonsense suppression
… modification (PTM) of proteins is lysine acetylation. This is an especially ubiquitous PTM in the histones of chromatin, and is important for helping to regulate both structural and mechanistic aspects of chromatin. A number of strategies exist for generating acetylated nucleosomes …
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Understanding the Posttranslational Regulation of the Response Regulator Rcsb and Acetyl Phosphate as an Acetyl Group Donor in E. Coli
<p>The observation that Nepsilon-lysine acetylation occurs on a hundreds of proteins in bacteria is a recent discovery. To study the mechanisms that regulate acetylation and to determine if acetylation affects physiology, I studied the Escherichia coli response regulator and transcription factor …
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The Substrate and Regulator of Acetyltransferase San
Lysine acetylation is one of the most common protein modifications in eukaryotes and plays critical roles in numerous cellular events. San is an acetyltransferase required for proper chromosome segregation in Drosophila and human, but little is known about its substrates or upstream regulators. To …
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Investigations Into the Function of Elp3 in Toxoplasma Gondii
… to the OMM. In other species, Elp3 mediates lysine acetylation of histones and alphatubulin, and its radical S-adenosyl methionine (rSAM) domain is important for the formation of tRNA modifications, which enhance translation efficiency and fidelity. Given its location, histones would not be …
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Chemical Mutagenesis for the Modulation and Study of Protein Function and Dysfunction within Alzheimer’s Disease
… accurate PTM mimetics for phosphorylation, lysine acetylation, and lysine dimethylation at diverse sites along tau. This approach produced homogenous products that accurately recapitulated known behaviours and allowed for the initial characterisation of poorly investigated PTMs. The …
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N⁶-formylation of lysine : a pathological secondary modification of proteins
… We recently discovered that N⁶-formylation of lysine is an abundant endogenous modification of histone and chromatin proteins. The high abundance of N⁶-formyllysine in histone proteins and its chemical similarity to the biologically important N⁶- acetyllysine has raised questions about its …
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LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF POST-TRANSLATIONAL MODIFICATIONS TO HMGB1 AND ITS REGULATED SECRETION IN LUNG CANCER
… modification (PTM), particularly acetylation and oxidation, mediate HMGB1 release and confer distinct extracellular HMGB1 signaling activity. However, the regulation of HMGB1 subcellular mobility in lung cancer by PTMs has not yet been established. To this end, a methodology was …
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Evolutionary, structural and functional features of cellular signalling networks
… components of PTM signalling systems to lysine acetylation. The proteins of this system were found to be similarly conserved as essential genes. Their evolutionary histories suggested a conserved origin in chromatin regulation, followed by functional diversification. Chapter 4 extends the …
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Multidrug resistance proteins (MRP) transport the cytokine/nuclear protein High Mobility Group Box 1 (HMGB1) across membranes
… first translocation step was already known to be lysine acetylation. Here I demonstrate the necessity of cysteine glutathionylation for the translocation inside secretory Iysosomes, and the direct involvement of Multidrug Resistance Proteins (MRP/ABCC) in the transport process. I started with the …
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Studio delle modificazioni post-traduzionali della proteina AIRE e dell’effetto sulla sua attività trascrizionale, stabilità e localizzazione cellulare
… which regulates DNA transcription through the acetylation and deacetylation of histones. Several evidences have shown that AIRE-CBP interaction occurs in nuclear bodies and increases the expression of its target genes (Akiyoshi H et al 2004), (Pitkänen J. et al 2005), (Ferguson BJ et al 2008). …
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Regulation of AMPA receptor acetylation and translation by SIRT2 and AMPK: the molecular mechanisms and implications in memory formation
… controls AMPAR stability by regulating AMPAR acetylation. For the first time, we discovered that AMPARs are subject to lysine acetylation, a novel form of post-translational modification for glutamate receptors. Under basal conditions, AMPARs are highly acetylated at their intracellular C …
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Proteome-Wide Prediction of Acetylation Substrates
… or by recruiting effector proteins to chromatin. Acetylation is one of the well-studied post-translational modifications that has been associated with chromatin-associated processes, notably gene regulation. Many studies have contributed to our knowledge of the enzymology underlying acetylation, …
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Metabolic Regulation of Gene Expression through Differential Histone Acylation: The Regulation and Function of Histone Crotonylation
<p>Histone lysine acetylation (Kac) plays a critical role in gene regulation by affecting the accessibility of the DNA wrapped around histones and by recruiting effector complexes. Three major classes of proteins are associated with Kac, namely "writers," enzymes that covalently modify specific …
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Superoxide via Sp3 Transcription Factor Up-Regulates Renal AT1 Receptor Function and Contributes to Hypertension
… increased diuresis and natriuresis. Importantly, lysine acetylation of Sp3 protein, an index of Sp3 activation, was significantly higher in DETC treated rats, which was attenuated with tempol treatment. Our data in both in vivo rat experiments and in vitro cell culture studies suggest that …
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C-terminal lysines modulate Connexin32 turnover and its ability to suppress growth of Neuro-2a cell cultures
… if post-translational modifications, such as lysine acetylation, regulate connexin function through the fine-tuning of protein turnover or some other aspect of GJ dynamics. We chose to focus specifically on Cx32 for several reasons. Cx32 is an important regulator of neuronal myelination and …