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Showing 1 to 20 of 35 for “"Lyophilisation"”.
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Production of Dermal Inspired Collagen Architectures by Lyophilisation and Electrophoretic Deposition
Collagen scaffolds have been used successfully as dermal regeneration templates in clinical applications, but most do not recapitulate the natural tissue structure and are often combined with synthetic membranes to afford additional barrier properties. This work aimed to produce dermal-inspired …
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Development of a Cytochrome P450 Expression System with Biomass Scale-up, Streamlined Downstream Processing and Stability Modifications
… and rapidly lose activity at room temperature. Lyophilisation is known to extend the shelf-life of recombinant proteins. Although lyophilisation is a harsh procedure which could shear the P450 proteins, from the microsomal membranes, it was found it could stabilise P450 activity so that the …
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Systematische Rezepturentwicklung von Tabletten aus Lyophilisaten - Grundlage für ein wissensbasiertes System
… dieser Stoffe untersucht werden. Nach der Lyophilisation ergaben sich Unterschiede bezüglich der Bruchfestigkeit der Tabletten: Die Substanzen zeigten vor allem bei niedrigem Pressdruck eine höhere Bruchfestigkeit als native Materialien. Insgesamt betrachtet ist die …
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Glucose-Oxidase-Liposomen zur Therapie der Septischen Granulomatose : in vitro Funktionalität, Granulozyten-Targeting und Wirkung im Organismus
… <br>Zunächst sollte die Zubereitungsform durch Lyophilisation stabilisiert werden. Es gelang nicht zu verhindern, dass während des Lyophilisations- und Redispergierungsprozesses 30-40 der in den Liposomen verkapselten GO freigesetzt wurden. Aus diesem Grund wurde von einer Lyophilisation …
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Design of oral insulin tablet formulations to enable manufacture and storage under ambient conditions
… to accelerate degradation. In contrast, lyophilisation (freeze-drying), a low-stress process commonly used in parenteral formulations, enables the formation of amorphous matrices that immobilise peptides, restricting molecular mobility and mitigating degradation pathways. This thesis …
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Development of a novel cyanide-free method for analysis of vitamin B₁₂ in milk-based infant formula
… affinity chromatography and concentrated by lyophilisation. α-Ribazole was identified and characterised by nuclear magnetic resonance spectroscopy and liquid chromatography mass spectrometry to demonstrate its suitability as a standard. This protocol was optimised and adopted in the sample …
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Low Frequency Dielectric Investigations on Hydrated Pharmaceutical Powders
… viz. tabletting (including granulation) and the lyophilisation of proteins. A previously poorly characterised low frequency dispersion in MCC is shown to be a bulk process (a separate dispersion due to the presence of the blocking electrodes was observed) that is almost certainly due to the …
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The development of physico-chemical quality control methods for Haemophilus influenzae type b vaccine production
… Samples were dried by means of lyophilisation and then subjected to methanolysis to yield methyl glycosides. A trimethylsilylating reagent was used to volatilise the analyte and analysis was performed using GC-FID with direct injection. The use of internal standards throughout …
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La nitrification dans le cadre du traitement des eaux usées: production de nitrites, préservation des nitrifiants et effet d'un support
… à plus long terme à -20°C, -80°C ou par lyophilisation, en présence ou en absence de cryoprotecteurs (DMSO et glycérol). Il apparaît que les traitements employés ont des effets significatifs sur les taux de production de nitrites plus nitrates (p<0.05). La lyophilisation ne semble pas …
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Formulation of targeted drug-loaded nanoparticles for lung cancer therapy
… entrapment efficiency and release studies, lyophilisation and invitro assay against A549 cells. It was also observed that these stable liposome formulations are suitable for entrapment of both cisplatin and irinotecan and demonstrated higher cytotoxicity against A549 cancer cells in …
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Formulation and characterisation of cationic microparticles for the delivery of DNA vaccines
… production were assessed by cryopreparation and lyophilisation studies (chapters 3 and 4). Gene expression levels in cells of the immune response were evaluated by microparticle transfection of the dendritic cell (DC) line 2.4 and primary bone marrow derived DCs (chapter 6). In vivo, mice were …
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Design, production and evaluation of an auxetic straining device for application in lung tissue engineering scaffolds
… First, a predictive relationship between the lyophilisation conditions and the resulting pore size of ice-templated collagen scaffolds was established. Systematic variations in the freeze-drying conditions resulted in scaffolds with average pore sizes ranging from 46 μm to 251 μm, closely …
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Isolation, Characterisation and Preservation of Bacteriophages for Use in Treatment of Aeromonas hydrophila Infection In Finfish
… Any changes in phage morphology after lyophilisation was determined by TEM. The therapeutic potential of isolated phages was assessed in fish trials with a native Australian fish, silver perch (Bidyanus bidyanus) against one strain of A. hydrophila. A cocktail of four selected phages …
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Towards Accessible Diagnostics: Developing CRISPR-Based Colorimetric Tools for Low- and Middle-Income Countries
… while remaining active for four weeks after lyophilisation at ambient temperature, facilitating the possibility of shipment without cold chain, significantly reducing deployment costs. Further simplification of this colorimetric two-step reaction was explored by replacing the full …
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Design and Development of Solid Lipid Nanoparticles for Ocular Delivery of Acyclovir
… (ACV-βCD) inclusion complexes were prepared by lyophilisation. Nanoparticles were coated with the biodegradable, cationic and bioadhesive polymer, chitosan and were characterised for cytotoxicity, cellular uptake, antiviral efficiency and stability. Finally, the chosen chitosan coated NLCs …
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Advancing protein-based biomaterials
… ± 59.4 Pa and 352.3 ± 102.0 Pa). Due to the lyophilisation process which led to the fragmentation of the original long fibrils into shorter rod-shaped structures, the fibril length proved inadequate to contribute to gel strength. The incorporation of ceramic particles into selected …
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Towards smart molecular biosensors: Modulation of DNA strand displacement reactions with transcription factors for robust and tunable arsenic quantification
… ability to withstand environmental variation and lyophilisation, while an improved mechanistic model could permit optimisation of component concentrations and properties. Combined with suitable hardware, a practical arsenic biosensor may be possible. Perhaps more importantly, however, this project …
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Zirconium-based metal-organic frameworks as drug delivery systems
… polyethylene glycol (mPEG-PO3) followed by lyophilisation. These PEGylated nanoMOFs were able to maintain their hydrodynamic diameter in water and PBS compared to their non-PEGylated counterparts. PEGylated nanoMOFs also reduced cytotoxicity in vitro and were taken up by HeLa cells within 24 …
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The Development and Characterisation of a Peptide Delivery Platform to Interrogate the Anticancer Effects of Bisphosphonates
… RALA/ALN nanoparticles remained stable post-lyophilisation without compromising on functionality in vitro. <br/><br/>RALA++, a MMP-2/MMP-9 cleavable PEG moiety, was employed in vivo to enhance tumour targeted specificity and to avoid clearance of RALA/ALN nanoparticles via the mononuclear …
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