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Showing 1 to 20 of 20 for “"Lyases"”.
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DNA catalysts as phosphatases and as phosphoserine lyases
… Separately, application of sequence-general lyases in the synthesis of complex lanthipeptides and enrichment of phosphopeptides/proteins in phosphoproteomics will be explored.
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Over-expression and characterization of four alginate lyases from vibrio splendidus 12B01
… a homo- or heteropolymeric structure. Alginate lyases are enzymes that degrade the linkage between G and M blocks and can have specificity to either polyG, polyM, or polyMG block degradation. The marine bacterium Vibrio splendidus 12B01 contains four putative alginate lyases which were …
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Identification and Characterization of a New Class of Bilin Lyases in Synechococcus sp. PCC 7002
… is to identify and characterize the enzymes (lyases) responsible for chromophore (bilin) attachment to phycobiliproteins (light harvesting) in cyanobacteria. Candidates for these lyases were first identified in Fremyella diplosiphon as cpeS and cpeT. In Synechococcus sp. PCC 7002, there are …
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Alginate and laminarin degrading enzymes from Vibrio splendidus and Vibrio breoganii
… and laminarin. These enzymes are called alginate lyases and laminarinases. V. splendidus 12B01, V. splendidus 13B01, and V. breoganii 1C10 each contain between four and twelve putative alginate lyases. We have over-expressed and purified 21 alginate lyases from these organisms and determined under …
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Structural studies on enzymes involved in uronic acid polysaccharide degradation
… These enzymes are called polysaccharide lyases (PL) and degrade the uronate polysaccharide by a β-elimination mechanism. Polysaccharide lyase enzymes are known to be involved in microbial pathogenicity and gut microbiome interactions. In addition, PL enzymes serve as biotechnology tools …
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On the molecular diversity of dimethylsulphoniopropionate catabolism by marine bacteria
… through the discovery of six different DMSP lyases and a DMSP demethylase, as well as downstream pathways. From these studies, it is becoming evident that there is great diversity in the way bacteria breakdown this important molecule. The work presented here further explores and expands our …
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Cloning and molecular characterisation of four alginate lyase genes from Vibrio midae SY9 : an enteric bacterium from the abalone Haliotis midae
… polymer; poly-M, poly-G or poly-MG. Alginate lyases are enzymes that utilize a -elimination reaction to depolymerise the alginate polymer resulting in cleavage of the (1-4)-O-glycosidic linkage between monomers and the formation of an unsaturated uronic acid at the new non-reducing terminus. …
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Determining and differentiating protein-biopolymer interactions
… sugars – Alginate and Laminarin by alginate lyases and laminarinases respectively. My work focuses on overcoming this limitation by characterizing several alginate lyases and laminarinases from 3 different bacteria (Vibrio splendidus 12B01, Vibrio splendidus 13B01 and Vibrio breoganii IC10) …
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Genetic analysis of DMSP metabolism in the marine Roseobacter clade
… (MMPA), and cleavage by DMSP-lyases, yielding volatile dimethylsulfide (DMS) plus acrylate. The DMSP-lyase, DddP, was purified from Roseovarius nubinhibens ISM and characterised in vitro. Nuclear magnetic resonance spectroscopy and gas chromatography confirmed bona fide DMSP …
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Characterization of Enzymes Involved in Bilin Attachment to Allophycocyanin in the Cyanobacterium Synechococcus sp. PCC 7002
… Synechococcus sp. PCC 7002. Candidates for lyases responsible for attachment of phycocyanobilin to allophycocyanin are two cpeS-like genes termed cpcS and cpcU, and one cpeT-like gene termed cpcT. In vitro bilin attachment reactions were conducted in the presence of the recombinant substrate …
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Identification and Characterization of Enzymes Involved in Post-translational Modifications of Phycobiliproteins in the Cyanobacterium Synechocystis sp. PCC 6803
… PBP assemble into trimers. Candidates for the lyases responsible for attachment of phycocyanobilin to phycocyanin in Synechocystis sp. PCC 6803 are two cpeS-like genes (cpcS and cpcU) and one cpeT-like gene (cpcT). Through absorbance and fluorescence spectra, it was determined that CpcS and …
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Understanding the catalytic machinery of chondroitinase ABC I in processing dermatan sulfate
… enzyme family is comprised of several bacterial lyases known to dissolve galactosaminoglycans in the extracellular matrix. Although several studies have shown the benefit of using chondroitinase enzymes for treatment, there is much to learn about its enzyme-substrate complex. For the purpose of …
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The chemistry and evolution of enzyme function: isomerases as a case study
… transferases (EC 5.4) and intramolecular lyases (EC 5.5) is challenging. The subclass \other isomerases" (EC 5.99) sits apart from other subclasses and exhibits great diversity. The current classification of isomerases in six subclasses reduces to two subclasses if the type of isomerism is …
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Genetic Analysis of Chondroitin Sulfate Utilization in Bacteroides Thetaiotaomicron (Cloning, Mutagenesis, Polysaccharide)
… produces two inducible chondroitin lyases (I and II) when it is grown on chondroitin sulfate. I have cloned in Escherichia coli the B. thetaiotaomicron gene which encodes for the production of chondroitin lyase II. This gene has been localized on the cloned fragment by (gamma)(delta) …
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Molecular mechanisms of pathogenesis incited by Erwinia carotovora subsp. carotovora
… and mediates the production of endo-pectate lyases with isoelectric points (pI) of 9.5 and 7.5 in strain HB101. The pI 9.5 enzyme is believed to be the major extracellular pectolytic enzyme in soft-rot while the pI 7.5 enzyme has no documented counterpart in EC14. Subclone and transposon tn5 …
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Management of wine aroma using biotechnical tools
… transporters, and in the expression of β-lyases. This resulted in a faster and higher metabolization of aroma precursors in the fermenting musts and in an increased concentration of some VOCs. Lastly, it was studied the impact on must composition and wine aroma of Aspergilopepsins-I …
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Identification of grapevine Pectate Lyase genes altering Berry Texture during ripening
… (PGs) and/or cleaved by pectate lyases (PLs), leading to pectin degradation and tissue softening. Previous studies have investigated the role of specific genes or gene families in regulating fruit firmness, particularly pectate lyases. For instance, a knock-out study in tomato …
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Discovery of novel transaminases from halophilic microorganisms
… with a number of enzymes, including hydrolases, lyases, oxidases and transaminases(TAms). TAms are PLP-dependent enzymes which catalyse the transfer of an a minegroup from an a minedonor toaketoneoraldehyde,resulting in a chiralamine. Their use has been successfully applied to the synthesis of …