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Showing 1 to 17 of 17 for “"LuxI"”.
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Role of region 4 of the sigma 70 subunit of RNA polymerase in transcriptional activation of the lux operon during quorum sensing
… box centered at position -42.5 relative to the luxI transcriptional start site. During transcriptional activation of the lux operon, LuxR is thought to function as an ambidextrous activator capable of making multiple contacts with RNA polymerase (RNAP). The specific role of region 4 of the …
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Genetic Analysis of the Quorum Sensing Regulator EsaR
… quorum sensing mechanism, homologous to the LuxI/R system. Although activation of the EPS encoding genes by EsaR occurs after it complexes to the AHL (3-oxo-C6-HSL), unlike the LuxI/R system, this activation occurs by a different mechanism. At low cell densities, dimerized EsaR acts as a …
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Identification of Quorum Sensing Genes in Halococcus Hamelinensis SP. Nov. 100Na1
… mechanism of quorum sensing, hence a putative luxI homolog. The annotation for transcriptional regulator could be a luxR homolog since LuxR protein is a transcriptional regulator. Future experiments will determine the actual luxI homologs, the structure of the signal, and the genetic regulation …
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The Functional Characterization of QSEC a Bacterial Adrenergic Receptor and the LuxR Homologue SDIA in EHEC
… lactones (AHL). Generally, in these systems, LuxI synthesizes the AHL that LuxR senses. EHEC does not encode a LuxI homologue, indicating that it can respond to AHLs through SdiA, but cannot produce them. EHEC uses SdiA to sense its environment through other AHL-producing bacteria. Microarray …
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Global characterization of oncogenic kinase signaling
Aberrant activation and expression of the proto-oncogene Src have been observed in various human malignancies. Despite major advancements in kinase-targeted cancer therapies, targeting Src has only achieved limited clinical success, thus highlighting the urgent need to better understand the complex …
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Public participation in shantytown transformation in China : a case study
Public participation is not a familiar concept in China but there is a growing demand from urban planners and policymakers in China to understand and utilize public participation tools. This research seeks to answer: How does the public participate in planning in China? What kind of participation …
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Development of Methods for Structural Characterization of Pantoea stewartii Quorum-Sensing Regulator EsaR
… lactone (AHL) molecule, produced by a LuxI homologue, accumulates in the environment. The LuxR proteins bind to their respective AHL when the ligand accumulates to sufficient levels. Once bound to AHL, the holoproteins usually become functional as transcriptional activators. However, …
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The quorum-sensing regulation of Vibrio fischeri : novel components of the autoinducer/LuxR regulatory circuit
… homoserine-Iactone signal molecules (luxI-dependent 30C6-HSL and the ainS-dependent C8-HSL) and the transcriptional activator LuxR regulate the luminescence system in a cell-density dependent manner by a process termed quorum sensing. In this study, five additional proteins whose …
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The Influence of Changes In theMolecular Structure of N-Acylhomoserine Lactones On the Complexation and Dna-Binding of Luxr Protein
… quorum-sensing system, the Vibrio fischeri LuxI/LuxR paradigm, was limited to molecular genetic approaches due to the formation of inclusion bodies when overexpressing LuxR in Escherichia coli. The addition of its native autoinducer, N-(3-oxohexanoyl)-homoserine lactone (3OC6-HSL), during …
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Development of nanoscale biosensors for cancer related proteases and blood-borne pathogens based on electrochemical and optical methods
A lot of materials exhibit novel properties when scaled down to nanoscale. Here we explore nanoelectrode arrays (NEAs) and nanoparticles in the application of high performance biosensors. We have developed an electrochemical (EC) method for measuring the activity of proteases using vertically …
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Identification of Quorum Sensing Genes in Hypersaline Microbial Mats
… regulatory proteins. Among them are LuxI and LuxR homologues; well characterized proteins in several bacterial isolates including Gram-negative and Gram-positive bacteria in laboratory settings under standard conditions. Recent studies have also reported the QS phenomenon in the …
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Experimental Methods in Support of the Development of a Computational Model for Quorum Sensing in Vibrio fischeri
… in two divergent transcriptional units: luxR and luxICDABEG. The latter contains the genes required for luminescence and the luxI gene necessary for synthesis of an N-acyl-homoserine lactone commonly called autoinducer (AI). The luxR gene codes for a transcriptional regulatory protein that …
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Molekulare Analyse des Biotin-regulatorischen Netzwerks Sinorhizobium meliloti durch Proteomanalyse, Expressionsstudien und Mutagenesen
… Transkriptions-regulator des luxI- bzw. luxR-Typs. Diese Expressionsdaten wurden durch weitere Experimente bestätigt. Aus dem Überstand von unter Biotin-limitierenden Bedingungen angezogener Kulturen ließen sich größere Mengen eines Acyl-Homoserin-Lactons extrahieren und nach …
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Multi-tiered Regulation of luxR Provides Precise Timing and Maintenance of the Quorum Sensing Response of Vibrio fischeri
… which is known to act directly on the luxR and luxI intergenic region as a repressor. This indicates that CsrA may bypass the upstream parts of the quorum-sensing regulatory cascade that lead to litR activation, so that LitR and LuxR may be regulated differently in response to certain …
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Structure/Function Analysis of the Quorum-sensing Regulator EsaR from the Plant Pathogen Pantoea stewartii
… are key to the process of quorum sensing, the LuxI and LuxR homologues EsaI and EsaR. Most LuxR homologues function as activators of transcription in the presence of their cognate acylated homoserine lactone signal (AHL). EsaR utilizes an AHL-response opposite of the majority of the LuxR …
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Gene Expression Control for Synthetic Patterning of Bacterial Populations and Plants
The development of shape in multicellular organisms has intrigued human minds for millenia. Empowered by modern genetic techniques, molecular biologists are now striving to not only dissect developmental processes, but to exploit their modularity for the design of custom living systems used in …