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Showing 1 to 20 of 38 for “"Luminol"”.
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Immobilized Luminol as a Chemiluminescent Reagent
… thesis investigates the first use of immobilized luminol chemiluminescence (CL) reagents in the flow injection analysis of hydrogen peroxide. The research reported here has progressed in two principal areas: first, the method development and characterization of immobilized luminol CL reagents and …
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Studies of the chemiluminescence of luminol.
Massachusetts Institute of Technology. Dept. of Chemistry. Thesis. 1969. Ph.D.
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Analytical Applications of Catalysis and Suppressed Catalysis of Luminol Chemiluminescence
Luminol chemiluminescence (CL) has been used in a variety of determinations of species which catalyze the luminol CL reaction. The research reported here examined analytical applications related to catalysis of the luminol CL reaction with respect to three primary areas.
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Effects of silver nanoparticles on photochemical processes focusing on luminol chemiluminescence
… complex decay constant and chemiluminescence of luminol and ferricyanide, were studied. The tryptophan fluorescence was slightly enhanced by smaller nanoparticles and showed slight quenching in the presence of larger nanoparticles. The tris (2,2’-bipyridyl ruthenium (II) chloride) complex was …
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Characteristics and applications of the enhanced HRP-catalyzed oxidation of luminol
… the most efficient chemiluminescent molecules is luminol. As a result of this, luminol has received much attention in chemical analysis. An analytically useful CL system which has also received much attention in recent years is the enhanced horseradish peroxidase (HRP)-catalyzed luminol reaction. …
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The Role of Metal Ions and Metallo-Complexes in Luminol Chemiluminescence (Catalysis)
Luminol chemiluminescence (CL) has been employed for the sensitive detection of various analytes such as certain transition metal ions and hydrogen peroxide (H(,2)O(,2)). Metal ions and metallo-complexes significantly enhance the light output of the luminol CL reaction (these species are generally …
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Applications of Electrocatalyzed Chemiluminescence of Luminol (Hydrogen Peroxide, Glucose, Electrogeneration, Flow Injection)
During the past several decades, the luminol chemiluminescence (CL) reaction has been successfully applied to the sensitive determination of a variety of analytes. The research reported here made use of electrochemical methods both to catalyze the luminol CL reaction and to generate reagents for …
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Investigation of the Luminol Chemiluminescence Reaction as a Post-Column Reaction Detector for HPLC
The luminol chemiluminescence (CL) reaction has been used as a post-column reaction detector for high performance liquid chromatography (HPLC). The research reported here has progressed in two principle areas: first, the development of a method for the determination of amino acids and other …
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Determination of hydrogen peroxide using immobilized chemiluminescence reagents and flow injection analysis
In this work, use of immobilized luminol for the CL determination of hydrogen peroxide using flow injection analysis (FIA) was investigated. Nylon was initially chosen as the immobilization support because of its chemical inertness. Luminol was covalently immobilized on the inside of nylon tubing …
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DETERMINATION OF TRACE METALS BY lON-CHROMATOGRAPHY WITH CHEMILUMINESCENCE DETECTION
… metal analysis, based on the highly sensitive luminol-peroxide chemiluminescence (CL) reaction have been developed and are described. A simple cation-exchange chromatographic system was developed and used to separate cobalt from other metal ions. These cobalt ions were detected using an …
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Development of nanoscale biosensors for cancer related proteases and blood-borne pathogens based on electrochemical and optical methods
… study, we modified nanoparticles with luminol and viral nucleic acid to develop chemiluminescence (CL) biosensors for blood-borne pathogens. Luminol-labeled 10-nm-diameter gold nanoparticles (GNPs) served as a nanocarrier for enhancing CL signal. The CL signal can be observed over 8 …
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The Luminometer: A Sensitive Instrument for Measuring Low Amounts of Oxygen, and Some Applications to Problems Involving the Measurement of Photosynthesis
… rate through a cuvette, then over a solution of luminol and potassium t-butoxide in dimethylsulfoxide (DMSO). The light generated by the chemiluminescent reaction of luminol with oxygen is measured with a photomultiplier. A constant rate of oxygen production by photosynthesis in the cuvette is …
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Flow Injection Determination of Sugars by Combining Immobilized Enzyme Reactors and Chemiluminescence Detection
… produced by chemiluminescent reaction with luminol has been widely used.
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FLOW SYSTEMS FOR CLINICAL ANALYSIS USING IMMOBILIZED ENZYMES AND CHEMILUMINESCENCE DETECTION (CHROMIUM, GLUCOSE)
… determination of Cr(III) and glucose, based on luminol CL were developed. The Cr(III) system used EDTA as a masking agent to eliminate interferences from other metal ions, making it selective for Cr(III). The FIA system for glucose determination in biological fluids was based on the use of …
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Investigations and Applications of Metal Ion Enhanced Chemiluminescence
… based on the suppression of the Co(II) enhanced luminol reaction is presented in this work. Compared to the classical ninhydrin reaction, this method is faster, can be run at room temperature without thermostatting, can determine secondary amino acids without varying the reaction or instrument, …
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CHEMILUMINESCENCE ANALYSIS BASED ON TWO PHASE REACTANT SYSTEMS
… intensity.</p><p>The reaction between luminol and hydrogen peroxide was used in a probe configuration. The reaction was catalyzed by peroxidase immobilized on one end of the fiber optic. A non-linear response for hydrogen peroxide was found at a pH of 9 and 10('-3) M luminol …
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Enhanced chemiluminescent immunoassay for staphylococcal enterotoxin B in foods
… the $\rho$-iodophenol enhanced oxidation of luminol by hydrogen peroxide as indicator reaction. The reaction was catalyzed by horseradish peroxidase (HRPO) conjugated to immunospecific anti-SEB immunoglobulin G (IgG). Addition of $\rho$-iodophenol to the reaction mixture resulted in $>$2,000 …
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Miniaturised analytical systems with chemiluminescence detection for environmental applications
… 3 documents the investigation of the luminol-cobalt(II) chemiluminescence reaction within a microfluidic device using the portable chemiluminescence detection system to produce a miniaturised analytical system for the determination of hydrogen peroxide in rainwater. Enhancement of the …
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Luminescent Systems for Genomics and Proteomics
… CHAPTER 1, Chemiluminescence The work on luminol-enhancer solution lead to a new luminol solution formulation with 1 order of magnitude lower detection limit for HRP. This technology was patented with Cyanagen brand and is now sold worldwide for Western Blot and ELISA applications. CHAPTER …
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Chemiluminescent reactions catalyzed by nanoparticles of gold, silver, and gold/silver alloys
… chemiluminescent reaction taking place between luminol and potassium ferricyanide. It was found that silver nanoparticles and alloy nanoparticles enhance the CL process when their sizes remained in the range of 30 nm to 50 nm. The data show that the intensity and rate of chemiluminescence were …
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